Figure 7
From: Reverse protection assay: a tool to analyze transcriptional rates from individual promoters
Monitoring of rrn16 3'-end processing. (A) Analysis of 3'-ends of rrn16 transcripts by Reverse RNase Protection Assay (RePro, lane 1) and RNase Protection Assay (RPA, lane 2). Molecular weight marker in nt is provided on the side (M, lane 3). The positions of probes, primers, and protected fragments are shown in (B). Scissor symbols denote RNA processing sites. Black boxes denote genes; small arrows indicate primers; the ball-ended arrow indicates the complementary RNA probe and the fully protected RNA fragment; the bar-ended line denotes the protected RNA fragment representing the processed mature rrn16 3'-end. Respective sizes are given in nucleotides (nt).