A simple and efficient method for isolating small RNAs from different plant species

Table 2 Concentrations* of LWM RNA samples obtained using the LiCl method with different modifications

Tissue	Modification	ng/μl	Average 260/280	Average 260/230	Yield (μg)
Cactus	1) LMW RNA enrichment from the supernatants obtained after precipitation of total RNA	382.30	1.82	1.81	7.65
	2) LiCl precipitating (overnight), followed by LMW RNA enrichment	527.75	1.81	1.90	10.56
	3) Addition of LiCl, directly followed by LMW RNA enrichment	291.42	1.66	1.35	5.83
	4) Enrichment of LMW RNA directly after the chloroform extraction without addition of LiCl	1008.42	2.02	1.04	20.17
Arabidopsis	1) LMW RNA enrichment from the supernatants obtained after precipitation of total RNA	582.52	1.68	1.23	11.65
	2) LiCl precipitating (overnight), followed by LMW RNA enrichment	333.68	1.66	1.21	6.67
	3) Addition of LiCl, directly followed by LMW RNA enrichment	609.38	1.53	1.13	12.19
	4) Enrichment of LMW RNA directly after the chloroform extraction without addition of LiCl	1418.77	1.53	0.96	28.38

*Yields and spectrophotometric A260/A230 and A260/A280 ratios of LMW RNA isolated with LiCl extraction method modifications from Prickly pear cactus and Arabidopsis. 0.1 g of tissue was used for the extraction and quantified using a Nanodrop spectrophotometer. LMW RNA was extracted two times in triplicate (6 independent samples).

ISSN: 1746-4811