Figure 2From: A pair of new BAC and BIBAC vectors that facilitate BAC/BIBAC library construction and intact large genomic DNA insert exchangeDNA analyses of randomly picked colonies showing copy number increases of the composite vectors pHZAUBAC1 and pHZAUBIBAC1. A. DNA analysis of randomly picked colonies transformed with pIndigoBac536 (lane 10), pIndigoBac536-S (lane 9) and the ligation product of pIndigoBac536-S and pGEM-4Z at the Hin dIII cloning site (pHZAUBAC1, lanes 1-8). DNA samples were prepared from 2 mL overnight cultures and were resuspended in 50 μL TE. Aliquots of 5 μL were digested with Hin dIII and were loaded onto a 1% agarose gel. pIndigoBAC536-S is 470 bp smaller than pIndigoBAC536. B. DNA analysis of randomly picked colonies transformed with BIBAC-S (lane 9) and the ligation product of BIBAC-S and pGEM-4Z at the Bam HI cloning site (pHZAUBIBAC1, lanes 1-8). DNA samples were prepared from 2 mL overnight cultures and were resuspended in 50 μL of TE. Aliquots of 5 μL were digested with Bam HI and were loaded onto a 1% agarose gel. The marker is lambda/Hin dIII.Back to article page