Table 3 Oligonucleotides used in this study
Primers | Sequences (5' - 3') | Genebank accession no. |
|---|---|---|
Primers used for amplification of genes from apple | ||
   rbcS(+) | 1GACGAGAAAGCAGAGAGAGA20 | L24497 |
   rbcS(-) | 500CGATGATACGGATGAAGGAT481 | L24497 |
   CPN60α(+) | 1248ACTAATGACTCWGCTGGYGA1267 | DL175273 |
   CPN60α(-) | 2637GTWGCRTTCTTKGCATCCTC2618 | DL175273 |
   actin(+) | 1TCCTTCGTCTTGACCTTGCT20 | DQ822466 |
   actin(-) | 245ACGGAATCTCTCAGCTCCAA226 | DQ822466 |
   EF-1α(+) | 45ATGACCCTGCCAAGGAGGCT64 | U80268 |
   EF-1α(-) | 644GAATCGACACAACATAAACT625 | U80268 |
   EXP2(+) | 52ATGGCTTGAGCTGCGGGTCT71 | AY083167 |
   EXP2(-) | 651ACAATTCACCCTTCACCATT632 | AY083167 |
   PIP1b(+) | 84ATGGAAGGCAAGGAAGAGGA103 | AB100870 |
   PIP1b(-) | 673AAAACGGTGTAGACGAGGAC654 | AB100870 |
Primers used for ALSV vector construction | ||
   rbcSXho(+) | TACATCTCGAG 50 GGTACCGTGGCTACAGTT67 | |
   rbcS201Bam(-) | TACATGGATCC 250 AGGAAGGTAAGAGAGGGT233 | |
   rbcS102Bam(-) | TACATGGATCC 151 ATTGCTTTTTCTGGTGAC134 | |
   rbcS51Bam(-) | TACATGGATCC 100 TGGAGCAACCATTCTGGC83 | |
   CPN60αXho(+) | TACATCTCGAG 1864 ACTGACCAGAAGATTTCA1871 | |
   CPN60αBam(-) | TACATGGATCC 2064 GAGGAGAGCCTTTCTCCG2047 | |
   actinXho(+) | TACATCTCGAG 15 CTTGCTGGTCGTGACCTC32 | |
   actinBam(-) | TACATGGATCC 215 TTGGCCATCGGGAAGCTC198 | |
   EF-1αXho(+) | TACATCTCGAG 179 CTTACCAAGGTTGACAGG196 | |
   EF-1αBam(-) | TACATGGATCC 379 CTCAACGCTCTTGATAAC362 | |
   EXP2Xho(+) | TACATCTCGAG 252 AGAGCTGGAATTGTTCCT269 | |
   EXP2Bam(-) | TACATGGATCC 452 CCAGTTCTGGCCCCAGTT435 | |
   PIP1bXho(+) | TACATCTCGAG 405 ACCGCCGGCATCTCCGGT422 | |
   PIP1bBam(-) | TACATGGATCC 605 ATGGGCAACAGAGTTGGC588 | |
   MdTFLXho(+) | TACATCTCGAG 40 ATGAAAAGAGCCTCGGAG57 | |
   MdTFLBam(-) | TACATGGATCC 204 CACCAAAGTAAAGAAAGA223 | |