Figure 2From: Efficient virus-induced gene silencing in apple, pear and Japanese pear using Apple latent spherical virus vectorsVIGS of rbcS gene in pear and Japanese pear by ALSV vector infection. (A) Leaf chlorosis in pear and Japanese pear (J. pear) seedlings infected with rbcS201-ALSV (left) and normal seedlings infected with wtALSV 45 dpi. (B) Northern blot hybridization analysis of rbcS- mRNA (a), ALSV-RNAs (b) and rbcS-siRNAs (c) from leaves of the pear and J. pear seedlings infected with rbcS201-ALSV. Lane 1, healthy control; lane 2, wtALSV-infected leaves; lanes 3, the seedlings infected with rbcS201-ALSV.Back to article page