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Figure 5 | Plant Methods

Figure 5

From: Agrobacterium-mediated transformation of safflower and the efficient recovery of transgenic plants via grafting

Figure 5

The use of a GFP visual marker to rapidly identify transgenic callus and shoots of safflower. (A) GFP expression in proximal end of the cotyledon two weeks after Agrobacterium infection. (B) Shoot bud initiation from the proximal end of cotyledon in the presence of 18 mg l-1 hygromycin after four weeks of co-cultivation (C-D) Transformed shoots regenerating on selection media in presence of 18 mg l-1 hygromycin (E) Visualization of GFP signals in guard cells of a transgenic leaf. (F) A non-transformed leaf/shoot showing no GFP signal and low background fluorescence.

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Plant Methods

ISSN: 1746-4811