EdU assay | BrdU immunolocalization | |
---|---|---|
EdU or BrdU labeling | 2 h* | 2 h* |
Fixation and washes | 15 min + 15 min | 15 min + 15 min |
Cell wall digestion and washes | - | 30 min + 15 min |
Plasma membrane permeabilization and washes | - | 30 min + 15 min |
DNase I digestion and washes | - | 30 min + 15 min |
EdU click reaction or BrdU antibody incubations | 30 min | 3 h + 25 min + 1 h |
Washing with DAPI and sample mounting | 20 min | 20 min |
Total experimental duration | 3 h 20 min | 9 h 30 min |