Skip to main content

Table 1 Numbers of molecules in 3-week-old rosette leaves of wild-type Arabidopsis.

From: Normalization using ploidy and genomic DNA copy number allows absolute quantification of transcripts, proteins and metabolites in cells

Sample numbers

#1

#2

#3

#4

Genomic copy number/μl

2.47 × 105

2.40 × 105

4.12 × 105

4.39 × 105

s.e.m

0.16 × 105

0.22 × 105

0.47 × 105

0.33 × 105

n

11

10

20

20

RBC-L number/μl

1.48 × 1013

1.57 × 1013

2.40 × 1013

2.53 × 1013

s.d.

0.05 × 1013

0.18 × 1013

0.36 × 1013

0.18 × 1013

n

4

4

4

4

1RBC-L number/genome

5.99 × 107

6.54 × 107

5.83 × 107

5.76 × 107

2RBC-L number/cell

2.60 × 108

2.84 × 108

2.54 × 108

2.51 × 108

3 RBC-L number/cell

2.63 × 10 8

   

s.d .

0.15 × 10 8

   

4 CV

5.70 × 10 -2

   

Chlorophyll number/μl

9.88 × 1013

10.01 × 1013

17.72 × 1013

19.78 × 1013

s.d.

0.81 × 1013

0.20 × 1013

0.15 × 1013

0.04 × 1013

n

2

2

2

2

5Chlorophyll number/genome

4.00 × 108

4.17 × 108

4.30 × 108

4.51 × 108

6Chlorophyll number/cell

1.74 × 109

1.81 × 109

1.84 × 109

1.96 × 109

3 Chlorophyll number/cell

1.85 × 10 9

   

s.d .

0.09 × 10 9

   

4 CV

4.86 × 10 -2

   
  1. 1(RBC-L number/genome) = (RBC-L number/μl)/(Genome copy number/μl)
  2. 2(RBC-L number/cell) = (RBC-L number/genome) × (Genome copy number/cell)
  3. = (RBC-L number/genome) × 4.35
  4. 3Mean of sample number 1 to 4
  5. 4Coefficient of variation (s.d./mean)
  6. 5(Chlorophyll number/genome) = (Chlorophyll number/μl)/(Genome copy number/μl)
  7. 6(Chlorophyll number/cell) = (Chlorophyll number/genome) × (Genome copy number/cell)
  8. = (Chlorophyll number/genome) × 4.35