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Figure 9 | Plant Methods

Figure 9

From: A simple, rapid method to isolate salt glands for three-dimensional visualization, fluorescence imaging and cytological studies

Figure 9

Optical sections of a side view salt gland showing the distribution of nuclei. (A-H) Positions of nuclei (purple pseudo-colour; 1-6) at different focal planes. (I) Corresponding bright-field image of the same salt gland under study. The salt gland was stained with Coulter® DNA-Prep Stain containing 8 μg ml-1 PI and images were captured using Zeiss LSM 510. Excitation and emission wavelengths were 543 nm and >560 nm, respectively. Bars = 5 μm.

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Plant Methods

ISSN: 1746-4811