Table 1 Effect of artificial base mismatches on the specificity of allele-specific PCR.
| Â | Â | Mismatching base | |||
|---|---|---|---|---|---|
| Â | Â | A | T | G | C |
Matching base | A | 0% | 50% | 60% | 60% |
| Â | Â | (0/10) | (5/10) | (6/10) | (6/10) |
| Â | T | 60% | 0% | 90% | 10% |
| Â | Â | (6/10) | (0/10) | (9/10) | (1/10) |
| Â | G | 50% | 90% | 0% | 40% |
| Â | Â | (5/10) | (9/10) | (0/10) | (4/10) |
| Â | C | 60% | 40% | 40% | 0% |
| Â | Â | (6/10) | (4/10) | (4/10) | (0/10) |