Figure 4From: Protocol: a rapid and economical procedure for purification of plasmid or plant DNA with diverse applications in plant biologyMiniprep DNA eluted from silica matrix at high concentration allows efficient transient gene expression assays. A, Cotyledon cells of Arabidopsis seedling expressing the WRKY29-GFP-MYC protein in the nucleus. Miniprep DNA (2 μg in 1.8 μl) was bombarded into 7-day-old Arabidopsis seedlings and the observation was made 12 h post biolistic bombardment. B, Arabidopsis mesophyll protoplasts expressing the WRKY29-GFP-MYC protein in the nucleus. Miniprep DNA (2.2 μg in 2 μl) was introduced into 4,000 Arabidopsis mesophyll protoplasts by PEG-mediated transfection and the observation was made 12 h after transfection. C, Maize mesophyll protoplasts expressing the WRKY29-GFP-MYC protein in the nucleus. Fifteen μg of miniprep DNA was used to transfect 106 maize protoplasts by electroporation and the observation was made 12 h after transfection. Scale bar = 20 μm.Back to article page