Figure 1From: Impact of ubiquitous inhibitors on the GUS gene reporter system: evidence from the model plants Arabidopsis, tobacco and rice and correction methods for quantitative assays of transgenic and endogenous GUSPercentage of inhibition in different organs of the model plants Arabidopsis, tobacco and rice. Extracts were obtained from 100 mg/ml of fresh tissue for all organs except for tobacco pollen, where 10 mg/ml were used. Substrate and commercial E. coli GUS were added directly to the plant extract in order to minimize extract dilution. Inhibition against commercial E. coli GUS was calculated by comparing the measured reaction rate with that assayed in the extraction buffer. Stem I and II refer to stem in primary and secondary growth, respectively. Values are the means ± standard deviation from three independent extractions.Back to article page