From: Tape-Arabidopsis Sandwich - a simpler Arabidopsis protoplast isolation method
Light condition | Method | Protoplast No. (×107) | Transformation efficiency (%)* | Broken (%) |
---|---|---|---|---|
Low | Cutting | 1.8 ± 0.3 | 64.8 ± 5.8 | 1.7 ± 2.9 |
Low | Tape | 1.4 ± 0.2 | 66.2 ± 8.4 | 3.2 ± 5.6 |
Optimal | Cutting | 3.7 ± 1.3 | 53.0 ± 4.0 | 24.9 ± 10.3 |
Optimal | Tape | 3.0 ± 0.3 | 60.7 ± 3.2 | 1.8 ± 3.3 |