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Table 3 Summary of the average transformation efficiencies obtained for the six experiments, three constructs and two timings of Agrobacterium inoculation using the improved protocol.

From: High-throughput Agrobacterium-mediated barley transformation

  

Average transformation efficiency

Experiments:

A

27.1%

 

B

25.7%

 

C

25.3%

 

D

19.6%

 

E

20.0%

 

F

28.0%

Constructs:

pBract216

21.5%

 

pBract215

27.3%

 

pBract217

25.4%

Timing of inoculation:

DA

26.1%

 

SD

24.0%

  1. DA = day after (embryos inoculated the day after isolation); SD = same day (embryos isolated and inoculated on the same day).