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Table 3 PCR primers for amplification of COSII markers previously mapped to tomato (S. lycopersicum) chromosome XI.

From: Reliable allele detection using SNP-based PCR primers containing Locked Nucleic Acid: application in genetic mapping

COSII marker

Primer (5'–3')

 
 

Forward

Reverse

C2_At5g09880

AGGGTCAAGCATATCCATGTGGAC

TCTCTTGTGCATGGCTTGTTGAGCa

C2_At5g04590

ATCACCACAGTCCTTGCACAGGG

AGGACAAAGTGGAAAAGCTGGGa

C2_At3g54470

TCCTGACTTTGGTTCTAAGCTTAGATCGa

TCAAATATTAAGAAGTTGTGCTTGTCTGC

  1. aPrimers that were also used in combination with the respective AS SPD primer for SNP genotyping (see text; SNP genotyping). Primer source: SOL Genomics Network [30].
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Plant Methods

ISSN: 1746-4811