| GFP-species | ||
---|---|---|---|
parameter | ratiometric pHluorin | ecliptic pHluorin | Pt -GFP |
real isosbestic point λiso | 426 nm (ex) | 495 nm (em) | 437 nm (ex) |
apparent isosbestic point | 428 nm (ex) | 489 nm (em) | 430 nm (ex) |
left peak WL (λ1 < λiso) | 395 nm (ex, abs) | 464 nm (em) | 390 nm (ex) |
right peak WL(λ2 > λiso) | 475 nm (ex) | 511 nm (em) | 502 nm (ex) |
Stokes-shift (λem - λabs) | 113 nm (33 nm) | 114 nm (35 nm) | 6 nm |
maximum WL (λmax) | 508 nm (em) | 397 nm (ex, abs) | 508 nm (em) |
Rmax (F(λ1)/F(λ2)) | 2.75 | 14 | 107 |
Rmin (F(λ1)/F(λ2)) | 0.3 | 0.5 | 4.2 |
Rmax - Rmin | 2.44 | 13.5 | 103 |
Rmax/Rmin | 8.7 | 27 | 26 |
apparent pK (λ1; λ2) | 6.9 | 7.6 | 7.3 |
Responsiveness (Δlog(R)·ΔpH) | 2.9 | 2.0 | 9.8 |
pH sensitivity (Sx) | 0.77 | 0.41 | 0.69 |
pH sensitivity (Sm) | 0.34 | 1.05 | 0.09 |