Figure 3

Rapid selection of phosphinothricin-resistant Arabidopsis thaliana Ws seedlings. a. Seedlings from Arabidopsis thaliana Ws T1 seed obtained from plants subjected to floral dip transformation using Agrobacterium tumefaciens strain GV3101 harbouring the binary plasmid pSKI015. Seeds were plated on 1% agar containing MS medium (including vitamins) and phosphinothricin at a concentration of 50 μM. Following a stratification period of 2 d at 4°C in the dark, seedlings were incubated for 6 h at 22°C in white light, followed by incubation at 22°C in the dark for 2 d and continuous white light for a further 24 h. b. Phosphinothricin-resistant Arabidopsis seedling after rapid selection procedure (left); phosphinothricin-susceptible seedling after rapid selection procedure (right). Size bars 1 cm.