Figure 3From: Combining metal oxide affinity chromatography (MOAC) and selective mass spectrometry for robust identification of in vivo protein phosphorylation sitesMS2 spectrum and the cognate neutral loss MS3 spectrum of the phosphopeptide LGYTGENGGGQSEpSPVKDETPR exemplifying the additional sequence information gained of an MS2 spectrum compared to a MS3 spectrum. A: MS2 spectrum (Xcorr: 2.424); B: MS3 spectrum (Xcorr: 3.248). Exact localisation of the phosphorylation site is only possible with the MS3 spectrum in this case. Only ions above the noise signal were annotated.Back to article page