Figure 2
From: A streamlined method for systematic, high resolution in situ analysis of mRNA distribution in plants
Arrangement of sections and organisation of probes: (A) Silicone isolators to position sections on slides. Positions are shown 1 to 8. (B) Sections placed in postions 1 to 8, dried and shown after removal of silicone isolator. (C) Sections after de-waxing step. (D) Sections in hybridisation chambers. A-H cross refers to plate position of the probes (F). (E) Sections after colour development. (F) Organisation of probes in 96 well plate. (G) Organisation of Arabidopsis sections showing larger format hybridisation chamber on lower slide.