Table 2 Summary of Agrobacterium strains and vectors used to investigate wheat transformation.
From: Review of methodologies and a protocol for the Agrobacterium-mediated transformation of wheat
Agrobacteriu m strain (binary vector) | Chromosomal background | Ti plasmid | Opine classification | Additional vir genes on binary or helper plasmids | Binary type | Selectable and scorable marker on T-DNA. (Promoter shown in parentheses) |
|---|---|---|---|---|---|---|
ABI (pMON18365) [17, 18] | C58 | Disarmed pTiC58 | Nopaline | pMON18365, none reported | normal-binary | npt II (E35S) GUS (E35S) |
C58C1 (pPTN155) [15] | C58 | Cured/disarmed? | Nopaline | pPTN155, none reported | normal-binary | npt II (35S) GUS (E35S) |
AGL1 (pAL154/156) [21] | C58, RecA | pTiBo542 ΔT-DNA | Succinamopine | pAL154, 15.2 Kb fragment from pTiBo542 [47], pAL156, none | super-binary | bar (Ubi1) GUS (Ubi1) |
AGL0 (pBGX1 and pTO134) [19] | C58 | pTiBo542 ΔT-DNA | Succinamopine | pBGX1, none reported pTO134, none reported | normal-binary | hpt (35S) gfp (Ubi1) bar (35S) sgfp S65T (35S) |
AGL1 (pDM805) | C58, RecA | pTiBo542 ΔT | Succinamopine | pDM805, none reported | normal-binary | bar (Ubi1) GUS (Act1) |
LBA4404 (pTOK233) [45] | Ach5 | DNA Disarmed pAL4404 | Octopine | pTOK233, extra copy of vir B, vir C and vir G from pTiBo542 47, [48] | super-binary | hpt (35S) GUS (35S) |
LBA4404 (pHK21) [20] | RecA Ach5 | Disarmed pAL4404 | Octopine | pHK21, extra copy of vir B, vir C and vir G from pTiBo542 [47] | super-binary | bar (Ubi1) GUS (Ubi1) |
AGL1 (pUNN-2) [46] | C58, RecA | pTiBo542 ΔT-DNA | Succinamopine | pUNN-2, none reported | normal-binary | npt II (Ubi1)) |
ABI (pMON30139 and others) [16] | C58 | Disarmed pTiC58 | Nopaline | pMON30139, none reported | normal-binary | aro A:CP4 (Act1) aro A:CP4 (e35S+ hsp intron) |