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  1. Viroid research generally relies on infectious cDNA clones that consist of dimers of the entire viroid sequence. At present, those dimers are generated by self-ligation of monomeric cDNA, a strategy that prese...

    Authors: Joan Marquez-Molins, Jose Antonio Navarro, Vicente Pallas and Gustavo Gomez

    Citation: Plant Methods 2019 15:87

    Content type: Methodology

    Published on:

  2. The primary sources of nitrogen for plants have been suggested to be nitrate (NO3) and ammonium (NH4+). However, when both nitrate and ammonium are simultaneously available to plants, it is very difficult to dif...

    Authors: Kaiyan Zhang, Yanyou Wu and Hongtao Hang

    Citation: Plant Methods 2019 15:86

    Content type: Research

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  3. Most bacteria are not culturable, but can be identified through molecular methods such as metagenomics studies. Due to specific metabolic requirements and symbiotic relationships, these bacteria cannot survive...

    Authors: Joseph Krystel, Qingchun Shi, Jefferson Shaw, Goutam Gupta, David Hall and Ed Stover

    Citation: Plant Methods 2019 15:85

    Content type: Methodology

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  4. Wheat is one of the most important staple source in the world for human consumption, animal feed and industrial raw materials. To deal with the global and increasing population demand, enhancing crop yield by ...

    Authors: Thang Duong Quoc Le, Camille Alvarado, Christine Girousse, David Legland and Anne-Laure Chateigner-Boutin

    Citation: Plant Methods 2019 15:84

    Content type: Research

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  5. Experimental determination of the extent and rate of transport of liquid humates supplied to plants is critical in testing physiological effects of such biostimulants which are often supplied as foliar sprays....

    Authors: Marcela Smilkova, Jiri Smilek, Michal Kalina, Martina Klucakova, Miloslav Pekar and Petr Sedlacek

    Citation: Plant Methods 2019 15:83

    Content type: Methodology

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  6. Phytophthora root rot (PRR) caused by P. medicaginis is a major soil borne disease in chickpea growing regions of Australia. Sources of resistance have been identified in both cultivated and wild Cicer species. H...

    Authors: Amritha Amalraj, Julian Taylor and Tim Sutton

    Citation: Plant Methods 2019 15:82

    Content type: Research

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  7. Karrikins (KARs) are plant growth regulators that promote seed germination and the subsequent growth and development of seedlings of many plant species. In nature they are generated and released by combustion ...

    Authors: Jakub Hrdlička, Tomáš Gucký, Ondřej Novák, Manoj Kulkarni, Shubhpriya Gupta, Johannes van Staden and Karel Doležal

    Citation: Plant Methods 2019 15:81

    Content type: Research

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  8. Plant protoplasts are basic plant cells units in which the pecto-cellulosic cell wall has been removed, but the plasma membrane is intact. One of the main features of plant cells is their strong plasticity, an...

    Authors: Kaori Sakai, Florence Charlot, Thomas Le Saux, Sandrine Bonhomme, Fabien Nogué, Jean-Christophe Palauqui and Jacques Fattaccioli

    Citation: Plant Methods 2019 15:79

    Content type: Methodology

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  9. Integrated breeding approaches such as combining marker-assisted selection and rapid line fixation through single-seed-descent, can effectively increase the frequency of desirable alleles in a breeding program...

    Authors: Juan David Arbelaez, Erwin Tandayu, Maria Ymber Reveche, Annalhea Jarana, Petra van Rogen, Line Sandager, Patrik Stolt, Enghwa Ng, Rajeev K. Varshney, Tobias Kretzschmar and Joshua Cobb

    Citation: Plant Methods 2019 15:78

    Content type: Methodology

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  10. Deep learning algorithms for automated plant identification need large quantities of precisely labelled images in order to produce reliable classification results. Here, we explore what kind of perspectives an...

    Authors: Michael Rzanny, Patrick Mäder, Alice Deggelmann, Minqian Chen and Jana Wäldchen

    Citation: Plant Methods 2019 15:77

    Content type: Research

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  11. Accurate estimation of heading date of paddy rice greatly helps the breeders to understand the adaptability of different crop varieties in a given location. The heading date also plays a vital role in determin...

    Authors: Sai Vikas Desai, Vineeth N. Balasubramanian, Tokihiro Fukatsu, Seishi Ninomiya and Wei Guo

    Citation: Plant Methods 2019 15:76

    Content type: Methodology

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  12. Partial and full root-zone drought stresses are two widely used methods to induce soil drying in plant container-culture experiments. Two methods might lead to different observational results in plant water re...

    Authors: Asfa Batool, Zheng-Guo Cheng, Nudrat Aisha Akram, Guang-Chao Lv, Jun-Lan Xiong, Ying Zhu, Muhammad Ashraf and You-Cai Xiong

    Citation: Plant Methods 2019 15:75

    Content type: Research

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  13. Green plants comprise two lineages: (1) the streptophytes that colonised land and (2) the chlorophytes that have adaptations to land but remained mostly aquatic. To better understand what made streptophytes so...

    Authors: Lenka Caisová and Timothy O. Jobe

    Citation: Plant Methods 2019 15:74

    Content type: Methodology

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  14. In-field measurement of yield and growth rate in pasture species is imprecise and costly, limiting scientific and commercial application. Our study proposed a LiDAR-based mobile platform for non-invasive veget...

    Authors: Kioumars Ghamkhar, Kenji Irie, Michael Hagedorn, Jeffrey Hsiao, Jaco Fourie, Steve Gebbie, Valerio Hoyos-Villegas, Richard George, Alan Stewart, Courtney Inch, Armin Werner and Brent Barrett

    Citation: Plant Methods 2019 15:72

    Content type: Research

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  15. Clearing methods allow relatively quick processing of plant material and examination of cellular structures by rendering tissues and organs translucent. They have been adapted for plant embryology, primarily t...

    Authors: Monika Kwiatkowska, Dariusz Kadłuczka, Maria Wędzony, Beata Dedicova and Ewa Grzebelus

    Citation: Plant Methods 2019 15:71

    Content type: Methodology

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  16. A suitable reference gene is an important prerequisite for guarantying accurate and reliable results in quantitative real-time PCR (qRT-PCR) analyses. However, there is no absolute universality in reference ge...

    Authors: Canbin Chen, Jingyu Wu, Qingzhu Hua, Noemi Tel-Zur, Fangfang Xie, Zhike Zhang, Jianye Chen, Rong Zhang, Guibing Hu, Jietang Zhao and Yonghua Qin

    Citation: Plant Methods 2019 15:70

    Content type: Methodology

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  17. Development of oligonucleotide probes facilitates chromosome identification via fluorescence in situ hybridization (FISH) in many organisms.

    Authors: Pei Du, Caihong Cui, Hua Liu, Liuyang Fu, Lina Li, Xiaodong Dai, Li Qin, Siyu Wang, Suoyi Han, Jing Xu, Bing Liu, Bingyan Huang, Fengshou Tang, Wenzhao Dong, Zengjun Qi and Xinyou Zhang

    Citation: Plant Methods 2019 15:69

    Content type: Research

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  18. The stomata of plants mainly regulate gas exchange and water dispersion between the interior and external environments of plants and play a major role in the plants’ health. The existing methods of stomata seg...

    Authors: Kexin Li, Jianping Huang, Wenlong Song, Jingtao Wang, Shuai Lv and Xiuwei Wang

    Citation: Plant Methods 2019 15:67

    Content type: Research

    Published on:

  19. Hyperspectral reflectance data in the visible, near infrared and shortwave infrared range (VIS–NIR–SWIR, 400–2500 nm) are commonly used to nondestructively measure plant leaf properties. We investigated the us...

    Authors: Yufeng Ge, Abbas Atefi, Huichun Zhang, Chenyong Miao, Raghuprakash Kastoori Ramamurthy, Brandi Sigmon, Jinliang Yang and James C. Schnable

    Citation: Plant Methods 2019 15:66

    Content type: Research

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  20. Polygonatum cyrtonema Hua (P. cyrtonema) is one of the most important herbs in traditional Chinese medicine. Polysaccharides in P. cyrtonema plants comprise a class of important secondary metabolites and exhibit ...

    Authors: Chenkai Wang, Daiyin Peng, Jinhang Zhu, Derui Zhao, Yuanyuan Shi, Shengxiang Zhang, Kelong Ma, Jiawen Wu and Luqi Huang

    Citation: Plant Methods 2019 15:65

    Content type: Research

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  21. The quality of forage plants is a crucial component of animal performance and a limiting factor in pasture based production systems. Key forage attributes that may require improvement include the sugar, lipid,...

    Authors: Paul R. Shorten, Shane R. Leath, Jana Schmidt and Kioumars Ghamkhar

    Citation: Plant Methods 2019 15:63

    Content type: Research

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  22. Woody tropical plants contain high levels of complex organic compounds that inhibit the chemical procedures needed to extract RNA or DNA, thus compromising downstream applications such as RNA sequencing and an...

    Authors: François F. Barbier, Tinashe G. Chabikwa, Muhammad U. Ahsan, Stacey E. Cook, Rosanna Powell, Milos Tanurdzic and Christine A. Beveridge

    Citation: Plant Methods 2019 15:62

    Content type: Methodology

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  23. Standard strategies to identify genomic regions involved in a specific trait variation are often limited by time and resource consuming genotyping methods. Other limiting pre-requisites are the phenotyping of ...

    Authors: Charlotte Prodhomme, Danny Esselink, Theo Borm, Richard G. F. Visser, Herman J. van Eck and Jack H. Vossen

    Citation: Plant Methods 2019 15:60

    Content type: Research

    Published on:

  24. Root-zone environment is considered difficult to analyze, particularly in interpreting interactions between environment and plant. Closed-loop soilless cultures have been introduced to prevent environmental po...

    Authors: Taewon Moon, Tae In Ahn and Jung Eek Son

    Citation: Plant Methods 2019 15:59

    Content type: Methodology

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  25. Vectors based on plant viruses are important tools for functional genomics, cellular biology, plant genome engineering and molecular farming. We previously reported on the construction of PepGFP2a, a viral vec...

    Authors: Fabiola Ruiz-Ramón, Raquel N. Sempere, Eduardo Méndez-López, M. Amelia Sánchez-Pina and Miguel A. Aranda

    Citation: Plant Methods 2019 15:58

    Content type: Research

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  26. With environmental deterioration, natural resource scarcity, and rapid population growth, mankind is facing severe global food security problems. To meet future needs, it is necessary to accelerate progress in...

    Authors: Liang Han, Guijun Yang, Huayang Dai, Hao Yang, Bo Xu, Heli Li, Huiling Long, Zhenhai Li, Xiaodong Yang and Chunjiang Zhao

    Citation: Plant Methods 2019 15:57

    Content type: Research

    Published on:

  27. Soybean mosaic virus (SMV), a Potyvirus, is the most prevalent viral pathogen of soybean that causes severe yield and seed quality reductions in world soybean production. So far, multiple resistance loci for diff...

    Authors: Hua Jiang, Kai Li and Junyi Gai

    Citation: Plant Methods 2019 15:56

    Content type: Methodology

    Published on:

  28. The advances of hyperspectral technology provide a new analytic means to decrease the gap of phenomics and genomics caused by the fast development of plant genomics with the next generation sequencing technolo...

    Authors: Dawei Sun, Haiyan Cen, Haiyong Weng, Liang Wan, Alwaseela Abdalla, Ahmed Islam El-Manawy, Yueming Zhu, Nan Zhao, Haowei Fu, Juan Tang, Xiaolong Li, Hongkun Zheng, Qingyao Shu, Fei Liu and Yong He

    Citation: Plant Methods 2019 15:54

    Content type: Research

    Published on:

  29. Tanshinones are diterpenoid compounds that are used to treat cardiovascular diseases. As current extraction methods for tanshinones are inefficient, there is a pressing need to improve the production of these ...

    Authors: Tao Wei, Yonghong Gao, Kejun Deng, Lipeng Zhang, Meiling Yang, Xiaopei Liu, Caiyan Qi, Chunguo Wang, Wenqin Song, Yong Zhang and Chengbin Chen

    Citation: Plant Methods 2019 15:53

    Content type: Research

    Published on:

  30. After the publication of our article [1], it was brought to our attention that in six places in the article we omitted to use quotation marks to show where the text has been directly used from the cited refere...

    Authors: R. Makanza, M. Zaman-Allah, J. E. Cairns, J. Eyre, J. Burgueño, Ángela Pacheco, C. Diepenbrock, C. Magorokosho, A. Tarekegne, M. Olsen and B. M. Prasanna

    Citation: Plant Methods 2019 15:52

    Content type: Correction

    Published on:

    The original article was published in Plant Methods 2018 14:49

  31. A robust circadian clock has been implicated in plant resilience, resource-use efficiency, competitive growth and yield. A huge number of physiological processes are under circadian control in plants including...

    Authors: Hannah Rees, Susan Duncan, Peter Gould, Rachel Wells, Mark Greenwood, Thomas Brabbs and Anthony Hall

    Citation: Plant Methods 2019 15:51

    Content type: Research

    Published on:

  32. Plant roots are complex, three-dimensional structures that play a central role in anchorage, water and nutrient acquisition, storage and interaction with rhizosphere microbes. Studying the development of the p...

    Authors: Diego D. J. Corona-Lopez, Sarah Sommer, Stephen A. Rolfe, Frank Podd and Bruce D. Grieve

    Citation: Plant Methods 2019 15:49

    Content type: Research

    Published on:

  33. The outcrossing rate is a key determinant of the population-genetic structure of species and their long-term evolutionary trajectories. However, determining the outcrossing rate using current methods based on ...

    Authors: Friederike Jantzen, Natalia Wozniak, Christian Kappel, Adrien Sicard and Michael Lenhard

    Citation: Plant Methods 2019 15:47

    Content type: Methodology

    Published on:

  34. Methods used to quantify protein from biological samples are often inaccurate with significant variability that requires care to minimize. The errors result from losses during protein preparation and purificat...

    Authors: Shrikaar Kambhampati, Jia Li, Bradley S. Evans and Doug K. Allen

    Citation: Plant Methods 2019 15:46

    Content type: Methodology

    Published on:

  35. Gene editing using CRISPR/Cas9 is a simple and powerful tool for elucidating genetic controls and for crop improvement and its use has been reported in a growing number of important food crops, including recen...

    Authors: Fiona M. Wilson, Kate Harrison, Andrew D. Armitage, Andrew J. Simkin and Richard J. Harrison

    Citation: Plant Methods 2019 15:45

    Content type: Research

    Published on:

  36. With the introduction of high-throughput multisensory imaging platforms, the automatization of multimodal image analysis has become the focus of quantitative plant research. Due to a number of natural and tech...

    Authors: Michael Henke, Astrid Junker, Kerstin Neumann, Thomas Altmann and Evgeny Gladilin

    Citation: Plant Methods 2019 15:44

    Content type: Methodology

    Published on:

  37. Efficient seed germination is a crucial task at the beginning of crop cultivation. Although boundaries of environmental parameters that should be maintained are well studied, fine-tuning can significantly impr...

    Authors: Artyom Nikitin, Ilia Fastovets, Dmitrii Shadrin, Mariia Pukalchik and Ivan Oseledets

    Citation: Plant Methods 2019 15:43

    Content type: Research

    Published on:

  38. Many agronomic traits have been bred into modern wheat varieties, but wheat (Triticum aestivum L.) continues to be vulnerable to heat stress, with high night-time temperature (HNT) stress shown to have large nega...

    Authors: Nathan T. Hein, Dan Wagner, Raju Bheemanahalli, David Šebela, Carlos Bustamante, Anuj Chiluwal, Mitchell L. Neilsen and S. V. Krishna Jagadish

    Citation: Plant Methods 2019 15:41

    Content type: Methodology

    Published on:

  39. Glandular trichomes found in vascular plants are called natural cell factories because they synthesize and store secondary metabolites in glandular cells. To systematically understand the metabolic processes i...

    Authors: Ayako Takemori, Taiken Nakashima, Hisashi Ômura, Yuki Tanaka, Keisuke Nakata, Hiroshi Nonami and Nobuaki Takemori

    Citation: Plant Methods 2019 15:40

    Content type: Methodology

    Published on:

  40. Guard cells perceive external and internal stimuli and regulate stomatal conductance in plants. With the use of gas exchange analyzers, time-resolved stomatal conductance responses to light intensity, [CO2] conce...

    Authors: Paulo H. O. Ceciliato, Jingbo Zhang, Qing Liu, Xin Shen, Honghong Hu, Chen Liu, Anton R. Schäffner and Julian I. Schroeder

    Citation: Plant Methods 2019 15:38

    Content type: Research

    Published on:

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