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  1. Field phenotyping by remote sensing has received increased interest in recent years with the possibility of achieving high-throughput analysis of crop fields. Along with the various technological developments,...

    Authors: Md Mehedi Hasan, Joshua P. Chopin, Hamid Laga and Stanley J. Miklavcic

    Citation: Plant Methods 2018 14:100

    Content type: Methodology

    Published on:

    The Correction to this article has been published in Plant Methods 2019 15:27

  2. Leaf cellular architecture plays an important role in setting limits for carbon assimilation and, thus, photosynthetic performance. However, the low density, fine structure, and sensitivity to desiccation of p...

    Authors: Andrew W. Mathers, Christopher Hepworth, Alice L. Baillie, Jen Sloan, Hannah Jones, Marjorie Lundgren, Andrew J. Fleming, Sacha J. Mooney and Craig J. Sturrock

    Citation: Plant Methods 2018 14:99

    Content type: Methodology

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  3. Expression of economically relevant proteins in alternative expression platforms, especially plant expression platforms, has gained significant interest in recent years. A special interest in working with plan...

    Authors: Israel A. Ramírez-Alanis, Justin B. Renaud, Silverio García-Lara, Rima Menassa and Guy A. Cardineau

    Citation: Plant Methods 2018 14:98

    Content type: Research

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  4. Improving plant water use efficiency (WUE) is a major target for improving crop yield resilience to adverse climate change. Identifying genetic variation in WUE usually relies on instantaneous measurements of ...

    Authors: Iván Jauregui, Shane A. Rothwell, Samuel H. Taylor, Martin A. J. Parry, Elizabete Carmo-Silva and Ian C. Dodd

    Citation: Plant Methods 2018 14:97

    Content type: Research

    Published on:

  5. The clear visualization of 3D organization at the cellular level in plant tissues is needed to fully understand plant development processes. Imaging tools allow the visualization of the main fluorophores and i...

    Authors: Charlotte Bureau, Nadège Lanau, Mathieu Ingouff, Boukhaddaoui Hassan, Anne-Cécile Meunier, Fanchon Divol, Rosie Sevilla, Delphine Mieulet, Anne Dievart and Christophe Périn

    Citation: Plant Methods 2018 14:96

    Content type: Methodology

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  6. An efficient transformation protocol is a primary requisite to study and utilize the genetic potential of any plant species. A quick transformation system is also crucial for the functional analysis of genes a...

    Authors: S. V. G. N. Priyadarshani, Bingyan Hu, Weimin Li, Hina Ali, Haifeng Jia, Lihua Zhao, Simon Peter Ojolo, Syed Muhammad Azam, Junjie Xiong, Maokai Yan, Zia ur Rahman, Qingsong Wu and Yuan Qin

    Citation: Plant Methods 2018 14:95

    Content type: Research

    Published on:

    The Correction to this article has been published in Plant Methods 2018 14:106

  7. The reduced growth of plants during the winter causes a lack in the perceptibility of the phenological events making challenging the study of dormancy. For deciduous crops, dormancy is generally determined by ...

    Authors: Hector Camargo Alvarez, Melba Salazar-Gutiérrez, Diana Zapata, Markus Keller and Gerrit Hoogenboom

    Citation: Plant Methods 2018 14:94

    Content type: Methodology

    Published on:

  8. Genetic transformation is a valuable tool and an important procedure in plant functional genomics contributing to gene discovery, allowing powerful insights into gene function and genetically controlled charac...

    Authors: Sadiye Hayta, Mark A. Smedley, Jinhong Li, Wendy A. Harwood and Philip M. Gilmartin

    Citation: Plant Methods 2018 14:93

    Content type: Methodology

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  9. Plasmopara obducens is the biotrophic oomycete responsible for impatiens downy mildew, a destructive disease of Impatiens that causes high crop loss. Currently, there are no available methods for the microscopic ...

    Authors: Catalina Salgado-Salazar, Gary R. Bauchan, Emma C. Wallace and Jo Anne Crouch

    Citation: Plant Methods 2018 14:92

    Content type: Methodology

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  10. The in vivo observation of diffusible components, such as ions and small phenolic compounds, remains a challenge in turgid plant organs. The analytical techniques used to localize such components in water-rich...

    Authors: Kevin Vidot, Cédric Gaillard, Camille Rivard, René Siret and Marc Lahaye

    Citation: Plant Methods 2018 14:89

    Content type: Methodology

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  11. Due to cowpea ability to fix nitrogen in poor soils and relative tolerance to drought and salt stresses, efforts have been directed to identifying genes and pathways that confer stress tolerance in this specie...

    Authors: Lidiane Lindinalva Barbosa Amorim, José Ribamar Costa Ferreira-Neto, João Pacífico Bezerra-Neto, Valesca Pandolfi, Flávia Tadeu de Araújo, Mitalle Karen da Silva Matos, Mauro Guida Santos, Ederson Akio Kido and Ana Maria Benko-Iseppon

    Citation: Plant Methods 2018 14:88

    Content type: Research

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  12. Production of virus-free plants is necessary to control viral diseases, import novel cultivars from other countries, exchange breeding materials between countries or regions and preserve plant germplasm. In vi...

    Authors: Min-Rui Wang, Zhen-Hua Cui, Jing-Wei Li, Xin-Yi Hao, Lei Zhao and Qiao-Chun Wang

    Citation: Plant Methods 2018 14:87

    Content type: Review

    Published on:

  13. Charcoal rot is a fungal disease that thrives in warm dry conditions and affects the yield of soybeans and other important agronomic crops worldwide. There is a need for robust, automatic and consistent early ...

    Authors: Koushik Nagasubramanian, Sarah Jones, Soumik Sarkar, Asheesh K. Singh, Arti Singh and Baskar Ganapathysubramanian

    Citation: Plant Methods 2018 14:86

    Content type: Research

    Published on:

  14. When developing CRISPR/Cas9 systems for crops, it is crucial to invest time characterizing the genome editing efficiency of the CRISPR/Cas9 cassettes, especially if the transformation system is difficult or ti...

    Authors: Lu Long, Dan-Dan Guo, Wei Gao, Wen-Wen Yang, Li-Pan Hou, Xiao-Nan Ma, Yu-Chen Miao, Jose Ramon Botella and Chun-Peng Song

    Citation: Plant Methods 2018 14:85

    Content type: Methodology

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  15. Root phenotyping aims to characterize root system architecture because of its functional role in resource acquisition. RGB imaging and analysis procedures measure root system traits via colour contrasts betwee...

    Authors: Gernot Bodner, Alireza Nakhforoosh, Thomas Arnold and Daniel Leitner

    Citation: Plant Methods 2018 14:84

    Content type: Methodology

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  16. Recently, putative pre-miRNAs locations have been identified in the introns of plant genes, raising the question whether such genes can show a dual functionality by having both correct maturation of the host g...

    Authors: Umidjon Shapulatov, Mark van Hoogdalem, Marielle Schreuder, Harro Bouwmeester, Ibrokhim Y. Abdurakhmonov and Alexander R. van der Krol

    Citation: Plant Methods 2018 14:83

    Content type: Research

    Published on:

  17. The paper introduces a multispectral imaging system and data-processing approach for the identification and discrimination of morphologically indistinguishable cryptic species of the destructive crop pest, the...

    Authors: Joseph Fennell, Charles Veys, Jose Dingle, Joachim Nwezeobi, Sharon van Brunschot, John Colvin and Bruce Grieve

    Citation: Plant Methods 2018 14:82

    Content type: Methodology

    Published on:

  18. Existing methods for directly measuring photosynthetic capacity (Amax) have low throughput, which creates a key bottleneck for pre-breeding and ecological research. Currently available commercial leaf gas exchang...

    Authors: William T. Salter, Matthew E. Gilbert and Thomas N. Buckley

    Citation: Plant Methods 2018 14:80

    Content type: Methodology

    Published on:

  19. Thapsigargin and nortrilobolide are sesquiterpene lactones found in the Mediterranean plant Thapsia garganica L. Thapsigargin is a potent inhibitor of the sarco/endoplasmic reticulum calcium ATPase pump, inducing...

    Authors: Carmen Quiñonero López, Patricia Corral, Bénédicte Lorrain-Lorrette, Karen Martinez-Swatson, Franck Michoux and Henrik Toft Simonsen

    Citation: Plant Methods 2018 14:79

    Content type: Research

    Published on:

  20. Progress in high-throughput molecular methods accompanied by more complex experimental designs demands novel data visualisation solutions. To specifically answer the question which parts of the specifical biol...

    Authors: Maja Zagorščak, Andrej Blejec, Živa Ramšak, Marko Petek, Tjaša Stare and Kristina Gruden

    Citation: Plant Methods 2018 14:78

    Content type: Software

    Published on:

  21. Wheat (Triticum aestivum L.) productivity is commonly limited by the availability of water. Increasing transpiration efficiency (biomass produced per unit of water used, TE) can potentially lead to increased grai...

    Authors: Andrew Fletcher, Jack Christopher, Mal Hunter, Greg Rebetzke and Karine Chenu

    Citation: Plant Methods 2018 14:77

    Content type: Methodology

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  22. The visible and near infrared region has been widely used to estimate the leaf nitrogen (N) content based on the correlation of N with chlorophyll and deep absorption valleys of chlorophyll in this region. How...

    Authors: Dong Li, Xue Wang, Hengbiao Zheng, Kai Zhou, Xia Yao, Yongchao Tian, Yan Zhu, Weixing Cao and Tao Cheng

    Citation: Plant Methods 2018 14:76

    Content type: Research

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  23. The recent development of next-generation sequencing DNA marker technologies, such as genotyping-by-sequencing (GBS), generates thousands of informative single nucleotide polymorphism markers in almost any spe...

    Authors: Craig B. Anderson, Benjamin K. Franzmayr, Soon Won Hong, Anna C. Larking, Tracey C. van Stijn, Rachel Tan, Roger Moraga, Marty J. Faville and Andrew G. Griffiths

    Citation: Plant Methods 2018 14:75

    Content type: Methodology

    Published on:

  24. Lignocellulosic biomass is a complex network of polymers making the cell walls of plants. It represents a feedstock of sustainable resources to be converted into fuels, chemicals and materials. Because of its ...

    Authors: Christine Terryn, Gabriel Paës and Corentin Spriet

    Citation: Plant Methods 2018 14:74

    Content type: Research

    Published on:

  25. We report the construction of a Virus-Induced Gene Silencing (VIGS) vector and an agroinoculation protocol for gene silencing in cassava (Manihot esculenta Crantz) leaves and roots. The African cassava mosaic vir...

    Authors: Ezequiel Matias Lentz, Joel-Elias Kuon, Adrian Alder, Nathalie Mangel, Ima M. Zainuddin, Emily Jane McCallum, Ravi Bodampalli Anjanappa, Wilhelm Gruissem and Hervé Vanderschuren

    Citation: Plant Methods 2018 14:73

    Content type: Methodology

    Published on:

  26. Sample preparation is a critical process for proteomic studies. Many efficient and reproducible sample preparation methods have been developed for mass spectrometry-based proteomic analysis of human and animal...

    Authors: Wei-Qing Wang, Ole Nørregaard Jensen, Ian Max Møller, Kim H. Hebelstrup and Adelina Rogowska-Wrzesinska

    Citation: Plant Methods 2018 14:72

    Content type: Research

    Published on:

  27. The next-generation sequencing (NGS) technology has greatly facilitated genomic and transcriptomic studies, contributing significantly in expanding the current knowledge on genome and transcriptome. However, t...

    Authors: Fu-Yuan Zhu, Mo-Xian Chen, Neng-Hui Ye, Wang-Min Qiao, Bei Gao, Wai-Ki Law, Yuan Tian, Dong Zhang, Di Zhang, Tie-Yuan Liu, Qi-Juan Hu, Yun-Ying Cao, Ze-Zhuo Su, Jianhua Zhang and Ying-Gao Liu

    Citation: Plant Methods 2018 14:69

    Content type: Methodology

    Published on:

  28. Full scent profiles emitted by living tissues can be screened by using total ion chromatograms generated in full scan mode and gas chromatography–mass spectrometry technique using Headspace Sorptive Extraction...

    Authors: Victoria Ruiz-Hernández, María José Roca, Marcos Egea-Cortines and Julia Weiss

    Citation: Plant Methods 2018 14:67

    Content type: Methodology

    Published on:

  29. High resolution and high throughput genotype to phenotype studies in plants are underway to accelerate breeding of climate ready crops. In the recent years, deep learning techniques and in particular Convoluti...

    Authors: Sarah Taghavi Namin, Mohammad Esmaeilzadeh, Mohammad Najafi, Tim B. Brown and Justin O. Borevitz

    Citation: Plant Methods 2018 14:66

    Content type: Methodology

    Published on:

  30. Our knowledge of natural genetic variation is increasing at an extremely rapid pace, affording an opportunity to come to a much richer understanding of how effects of specific genes are dependent on the geneti...

    Authors: Rui Wu, Miriam Lucke, Yun-ting Jang, Wangsheng Zhu, Efthymia Symeonidi, Congmao Wang, Joffrey Fitz, Wanyan Xi, Rebecca Schwab and Detlef Weigel

    Citation: Plant Methods 2018 14:65

    Content type: Research

    Published on:

  31. The images of different flower species had small inter-class variations across different classes as well as large intra-class variations within a class. Flower classification techniques are mainly based on the...

    Authors: Ping Lin, Du Li, Zhiyong Zou, Yongming Chen and Shanchao Jiang

    Citation: Plant Methods 2018 14:64

    Content type: Research

    Published on:

  32. The model species Arabidopsis thaliana has extensive resources to investigate intraspecific trait variability and the genetic bases of ecologically relevant traits. However, the cost of equipment and software req...

    Authors: François Vasseur, Justine Bresson, George Wang, Rebecca Schwab and Detlef Weigel

    Citation: Plant Methods 2018 14:63

    Content type: Methodology

    Published on:

  33. Metabolomics based on mass spectrometry analysis are increasingly applied in diverse scientific domains, notably agronomy and plant biology, in order to understand plants’ behaviors under different stress cond...

    Authors: Hikmat Ghosson, Adrián Schwarzenberg, Frank Jamois and Jean-Claude Yvin

    Citation: Plant Methods 2018 14:62

    Content type: Methodology

    Published on:

  34. Rhizobium rhizogenes transformation is commonly used to generate transgenic roots traditionally called hairy roots, for both investigative and commercial applications. While fertile plants can be regenerated from...

    Authors: Pradeepa C. G. Bandaranayake and John I. Yoder

    Citation: Plant Methods 2018 14:61

    Content type: Methodology

    Published on:

  35. Matrix-assisted laser-desorption and ionisation time-of-flight mass spectroscopy (MALDI-TOF MS) is a powerful tool for the characterisation and/or identification of protein-containing samples. Several MALDI-TO...

    Authors: Michael A. Reeve, Kathryn M. Pollard and Daisuke Kurose

    Citation: Plant Methods 2018 14:60

    Content type: Methodology

    Published on:

  36. In bioenergy/forage sorghum, morpho-anatomical stem properties are major components affecting standability and juice yield. However, phenotyping these traits is low-throughput, and has been restricted by the l...

    Authors: Francisco E. Gomez, Geraldo Carvalho Jr., Fuhao Shi, Anastasia H. Muliana and William L. Rooney

    Citation: Plant Methods 2018 14:59

    Content type: Methodology

    Published on:

  37. Unfortunately, in the original version [1] of this article, a funder note was missed out in the acknowledgement.

    Authors: Abelardo Montesinos-López, Osval A. Montesinos-López, Gustavo de los Campos, José Crossa, Juan Burgueño and Francisco Javier Luna-Vazquez

    Citation: Plant Methods 2018 14:57

    Content type: Correction

    Published on:

    The original article was published in Plant Methods 2018 14:46

  38. Small GTPases act as molecular switches that regulate various plant responses such as disease resistance, pollen tube growth, root hair development, cell wall patterning and hormone responses. Thus, to monito...

    Authors: Hann Ling Wong, Akira Akamatsu, Qiong Wang, Masayuki Higuchi, Tomonori Matsuda, Jun Okuda, Ken-ichi Kosami, Noriko Inada, Tsutomu Kawasaki, Takako Kaneko-Kawano, Shingo Nagawa, Li Tan, Yoji Kawano and Ko Shimamoto

    Citation: Plant Methods 2018 14:56

    Content type: Methodology

    Published on:

  39. Chickpea (Cicer arietinum L.), an important legume crop is one of the major source of dietary protein. Developing an efficient and reproducible transformation method is imperative to expedite functional genomics...

    Authors: Pooja Rani Aggarwal, Papri Nag, Pooja Choudhary, Niranjan Chakraborty and Subhra Chakraborty

    Citation: Plant Methods 2018 14:55

    Content type: Methodology

    Published on:

  40. In studies of plant stress signaling, a major challenge is the lack of non-invasive methods to detect physiological plant responses and to characterize plant–plant communication over time and space.

    Authors: Leandro do Prado Ribeiro, Adriana Lídia Santana Klock, João Américo Wordell Filho, Marco Aurélio Tramontin, Marília Almeida Trapp, Axel Mithöfer and Christian Nansen

    Citation: Plant Methods 2018 14:54

    Content type: Research

    Published on:

  41. Plant height is an important morphological and developmental phenotype that directly indicates overall plant growth and is widely predictive of final grain yield and biomass. Currently, manually measuring plan...

    Authors: Xu Wang, Daljit Singh, Sandeep Marla, Geoffrey Morris and Jesse Poland

    Citation: Plant Methods 2018 14:53

    Content type: Research

    Published on:

  42. Plant cell walls are nanocomposites based on cellulose microfibrils embedded in a matrix of polysaccharides and aromatic polymers. They are optimized for different functions (e.g. mechanical stability) by chan...

    Authors: Batirtze Prats-Mateu, Martin Felhofer, Anna de Juan and Notburga Gierlinger

    Citation: Plant Methods 2018 14:52

    Content type: Research

    Published on:

  43. Metabolic fluxes represent the functional phenotypes of biochemical pathways and are essential to reveal the distribution of precursors among metabolic networks. Although analysis of metabolic fluxes, facilit...

    Authors: Peng Wang, Longyun Guo, Rohit Jaini, Antje Klempien, Rachel M. McCoy, John A. Morgan, Natalia Dudareva and Clint Chapple

    Citation: Plant Methods 2018 14:51

    Content type: Research

    Published on:

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