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  1. To study the impact of climate warming on crops, it is crucial to have a warming equipment suitable for their field environment. A facility is needed that can provide suitable combinations of different tempera...

    Authors: Zuolin Zhang, Zhiyuan Yang, Shah Fahad, Tong Zhang, Wenhao Xu, Kehui Cui, Shaobing Peng and Jianliang Huang

    Citation: Plant Methods 2020 16:57

    Content type: Research

    Published on:

  2. Wild apple, Malus sieversii, is an endangered species and a valuable genetic resource that requires a variety of conservation techniques. This study aimed to investigate the influence of different concentrations ...

    Authors: Y. Zhang, T. A. Bozorov, D. X. Li, P. Zhou, X. J. Wen, Y. Ding and D. Y. Zhang

    Citation: Plant Methods 2020 16:56

    Content type: Research

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  3. To ensure further genetic gain, genomic approaches in plant breeding rely on precise phenotypic data, describing plant structure, function and performance. A more precise characterization of the environment wi...

    Authors: Andreas Honecker, Henrik Schumann, Diana Becirevic, Lasse Klingbeil, Kai Volland, Steffi Forberig, Marc Jansen, Hinrich Paulsen, Heiner Kuhlmann and Jens Léon

    Citation: Plant Methods 2020 16:55

    Content type: Software

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  4. Plant hormones are naturally occurring small molecule compounds which are present at trace amounts in plant. They play a pivotal role in the regulation of plant growth. The biological activity of plant hormone...

    Authors: Liyuan Wang, Yilin Zou, Han Yeong Kaw, Gang Wang, Huaze Sun, Long Cai, Chengyu Li, Long-Yue Meng and Donghao Li

    Citation: Plant Methods 2020 16:54

    Content type: Review

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  5. Phytoplasma are obligate intracellular plant-pathogenic bacteria that infect a broad range of plant species and are transmitted by different insect species. Quantitative real-time PCR (qPCR) is one of the most...

    Authors: Cecilia Mittelberger, Lisa Obkircher, Vicky Oberkofler, Alan Ianeselli, Christine Kerschbamer, Andreas Gallmetzer, Yazmid Reyes-Dominguez, Thomas Letschka and Katrin Janik

    Citation: Plant Methods 2020 16:53

    Content type: Methodology

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  6. Functional genomic studies using genetics approaches of conifers are hampered by the complex and enormous genome, long vegetative growth period, and exertion in genetic transformation. Thus, the research carri...

    Authors: Shuangwei Liu, Jingjing Ma, Hongmei Liu, Yingtian Guo, Wei Li and Shihui Niu

    Citation: Plant Methods 2020 16:52

    Content type: Methodology

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  7. Rain-induced fruit cracking is a major physiological problem in most sweet cherry cultivars. For an in vivo cracking assay, the ‘Christensen method’ (cracking evaluation following fruit immersion in water) is ...

    Authors: Michail Michailidis, Evangelos Karagiannis, Georgia Tanou, Eirini Sarrou, Katerina Karamanoli, Athina Lazaridou, Stefan Martens and Athanassios Molassiotis

    Citation: Plant Methods 2020 16:51

    Content type: Research

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  8. The genus Utricularia belongs to Lentibulariaceae, the largest family of carnivorous plants, which includes terrestrial, epiphytic and aquatic species. The development of specialized structures that evolved for c...

    Authors: A. Oropeza-Aburto, S. A. Cervantes-Pérez, V. A. Albert and L. Herrera-Estrella

    Citation: Plant Methods 2020 16:50

    Content type: Methodology

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  9. Quantitative and qualitative assessment of visual and morphological traits of seed is slow and imprecise with potential for bias to be introduced when gathered with handheld tools. Colour, size and shape trait...

    Authors: Keith Halcro, Kaitlin McNabb, Ashley Lockinger, Didier Socquet-Juglard, Kirstin E. Bett and Scott D. Noble

    Citation: Plant Methods 2020 16:49

    Content type: Methodology

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  10. Many methodological approaches have focused so far on physiological and molecular responses of plant tissues to freezing but only little knowledge is available on the consequences of extracellular ice-formatio...

    Authors: Othmar Buchner, Philip Steiner, Ancuela Andosch, Andreas Holzinger, Matthias Stegner, Gilbert Neuner and Ursula Lütz-Meindl

    Citation: Plant Methods 2020 16:48

    Content type: Methodology

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  11. Crocus sativus is a recalcitrant plant for genetic transformation and genetic improvement, largely due to difficulties in Agrobacterium mediated transformation and vegetative reproduction. Effective genome editin...

    Authors: Sudha Chib, Arulprakash Thangaraj, Sanjana Kaul, Manoj Kumar Dhar and Tanushri Kaul

    Citation: Plant Methods 2020 16:47

    Content type: Research

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  12. Stable isotopically labelled organisms have found wide application in life science research including plant physiology, plant stress and defense as well as metabolism related sciences. Therefore, the reproduci...

    Authors: Asja Ćeranić, Maria Doppler, Christoph Büschl, Alexandra Parich, Kangkang Xu, Andrea Koutnik, Hermann Bürstmayr, Marc Lemmens and Rainer Schuhmacher

    Citation: Plant Methods 2020 16:46

    Content type: Methodology

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  13. Short read sequencing technologies, such as genotyping-by-sequencing (GBS), have been utilized in genetic mapping, marker development, and population genomic studies. High-throughput and multiplexing capabilit...

    Authors: Narinder Singh, John Raupp, Dal-Hoe Koo, Bernd Friebe, Bikram Gill and Jesse Poland

    Citation: Plant Methods 2020 16:45

    Content type: Methodology

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  14. Brassica napus is an important oilseed crop that offers a considerable amount of biomass for global vegetable oil production. The establishment of an efficient genetic transformation system with a convenient tran...

    Authors: Kai Zhang, Jianjie He, Lu Liu, Runda Xie, Lu Qiu, Xicheng Li, Wenjue Yuan, Kang Chen, Yongtai Yin, May Me Me Kyaw, Aye Aye San, Shisheng Li, Xianying Tang, Chunhua Fu and Maoteng Li

    Citation: Plant Methods 2020 16:43

    Content type: Research

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  15. Reactive oxygen species (ROS) such as hydrogen peroxide and superoxide anions significantly accumulate during biotic and abiotic stress and cause oxidative damage and eventually cell death. There is accumulati...

    Authors: Lorenzo Fimognari, Rebecca Dölker, Greta Kaselyte, Camilla N. G. Jensen, Saqib S. Akhtar, Dominik K. Großkinsky and Thomas Roitsch

    Citation: Plant Methods 2020 16:42

    Content type: Methodology

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  16. Small RNAs are sequence-dependent negative regulators of gene expression involved in many relevant plant processes such as development, genome stability, or stress response. Functional characterization of sRNA...

    Authors: Diego López-Márquez, Ángel Del-Espino, Eduardo R. Bejarano, Carmen R. Beuzón and Javier Ruiz-Albert

    Citation: Plant Methods 2020 16:41

    Content type: Methodology

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  17. High throughput non-destructive phenotyping is emerging as a significant approach for phenotyping germplasm and breeding populations for the identification of superior donors, elite lines, and QTLs. Detection ...

    Authors: Tanuj Misra, Alka Arora, Sudeep Marwaha, Viswanathan Chinnusamy, Atmakuri Ramakrishna Rao, Rajni Jain, Rabi Narayan Sahoo, Mrinmoy Ray, Sudhir Kumar, Dhandapani Raju, Ranjeet Ranjan Jha, Aditya Nigam and Swati Goel

    Citation: Plant Methods 2020 16:40

    Content type: Methodology

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  18. The plant endophytic fungus Serendipita indica colonizes roots of a wide range of plant species and can enhance growth and stress resistance of these plants. Due to its ease of axenic cultivation and its broad ho...

    Authors: Mohamed Osman, Christian Stigloher, Martin J. Mueller and Frank Waller

    Citation: Plant Methods 2020 16:39

    Content type: Methodology

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  19. Non-destructive high-throughput plant phenotyping is becoming increasingly used and various methods for growth analysis have been proposed. Traditional longitudinal or repeated measures analyses that model gro...

    Authors: Chris Brien, Nathaniel Jewell, Stephanie J. Watts-Williams, Trevor Garnett and Bettina Berger

    Citation: Plant Methods 2020 16:36

    Content type: Methodology

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  20. Panicle density of cereal crops such as wheat and sorghum is one of the main components for plant breeders and agronomists in understanding the yield of their crops. To phenotype the panicle density effectivel...

    Authors: Akshay L. Chandra, Sai Vikas Desai, Vineeth N. Balasubramanian, Seishi Ninomiya and Wei Guo

    Citation: Plant Methods 2020 16:34

    Content type: Methodology

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  21. To understand processes regulating nutrient homeostasis at the single-cell level there is a need for new methods that allow multi-element profiling of biological samples ultimately only available as isolated t...

    Authors: Anle Chen, Thomas H. Hansen, Lene I. Olsen, Michael Palmgren, Søren Husted, Jan K. Schjoerring and Daniel Pergament Persson

    Citation: Plant Methods 2020 16:31

    Content type: Research

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  22. To investigate potential effects of herbicide phytotoxic on crops, a major challenge is a lack of non-destructive and rapid methods to detect plant growth that could allow characterization of herbicide-resista...

    Authors: Jian Wang, Chu Zhang, Ying Shi, Meijuan Long, Faisal Islam, Chong Yang, Su Yang, Yong He and Weijun Zhou

    Citation: Plant Methods 2020 16:30

    Content type: Research

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  23. Convolvulus sepium (hedge bindweed) detection in sugar beet fields remains a challenging problem due to variation in appearance of plants, illumination changes, foliage occlusions, and different growth stages und...

    Authors: Junfeng Gao, Andrew P. French, Michael P. Pound, Yong He, Tony P. Pridmore and Jan G. Pieters

    Citation: Plant Methods 2020 16:29

    Content type: Research

    Published on:

  24. Seed size and number are important plant traits from an ecological and horticultural/agronomic perspective. However, in small-seeded species such as Arabidopsis thaliana, research on seed size and number is limit...

    Authors: Alejandro Morales, J. Teapal, J. M. H. Ammerlaan, X. Yin, J. B. Evers, N. P. R. Anten, R. Sasidharan and M. van Zanten

    Citation: Plant Methods 2020 16:27

    Content type: Methodology

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  25. Biomacromolecules, the first components of bioactive substances in traditional Chinese medicines (TCM) have wide bioactivity-related efficacy but have not yet been fully appreciated compared to small molecule ...

    Authors: Wen-Jin Zhang, Sheng Wang, Chuan-zhi Kang, Chao-geng Lv, Li Zhou, Lu-Qi Huang and Lan-Ping Guo

    Citation: Plant Methods 2020 16:26

    Content type: Review

    Published on:

  26. Exogenous application of gamma-aminobutyric acid (GABA) could relieve stress symptoms caused by abiotic stresses including anoxia, heat and cold shock, drought, and salt. However, studying translocation and metab...

    Authors: Faraj Hijaz and Nabil Killiny

    Citation: Plant Methods 2020 16:24

    Content type: Research

    Published on:

  27. Image processing techniques have been widely used in the analysis of leaf characteristics. Earlier techniques for processing digital RGB color images of plant leaves had several drawbacks, such as inadequate d...

    Authors: Zhengmeng Chen, Fuzheng Wang, Pei Zhang, Chendan Ke, Yan Zhu, Weixing Cao and Haidong Jiang

    Citation: Plant Methods 2020 16:23

    Content type: Research

    Published on:

  28. Aneuploidy, a condition caused by an imbalance between the relative dosages of chromosomes, generally produces a novel phenotype specific to the molecular karyotype. Few techniques are currently available for ...

    Authors: Guo Wen, Jiangbo Dang, Zhongyi Xie, Jinying Wang, Pengfei Jiang, Qigao Guo and Guolu Liang

    Citation: Plant Methods 2020 16:22

    Content type: Methodology

    Published on:

  29. Myxospermy is a process by which the external surfaces of seeds of many plant species produce mucilage—a polysaccharide-rich gel with numerous fundamental research and industrial applications. Due to its funct...

    Authors: James M. Cowley, Lina Herliana, Kylie A. Neumann, Silvano Ciani, Virna Cerne and Rachel A. Burton

    Citation: Plant Methods 2020 16:20

    Content type: Research

    Published on:

  30. Tea, which is produced from new shoots of existing tea plants (Camellia sinensis), is one of the most popular, non-alcoholic, healthy beverages worldwide. Colletotrichum camelliae is one of the dominant fungal pa...

    Authors: Shengnan He, Huchen Chen, Yi Wei, Tai An and Shouan Liu

    Citation: Plant Methods 2020 16:17

    Content type: Methodology

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  31. Forward genetic screens are a powerful approach for identifying the genes contributing to a trait of interest. However, mutants arising in genes already known can obscure the identification of new genes contri...

    Authors: Jiangling Cao, Nial R. Gursanscky, Stephen J. Fletcher, Anne Sawyer, Mehershad Wadia, Lachlan McKeough, Marek Coleman, Uwe Dressel, Christelle Taochy, Neena Mitter, Hervé Vaucheret and Bernard J. Carroll

    Citation: Plant Methods 2020 16:16

    Content type: Methodology

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  32. Improving abiotic stress tolerance in wheat requires large scale screening of yield components such as seed weight, seed number and single seed weight, all of which is very laborious, and a detailed analysis o...

    Authors: Jessica Schmidt, Joelle Claussen, Norbert Wörlein, Anja Eggert, Delphine Fleury, Trevor Garnett and Stefan Gerth

    Citation: Plant Methods 2020 16:15

    Content type: Research

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  33. Plants, like all living organisms, metamorphose their bodies during their lifetime. All the developmental and growth events in a plant’s life are connected to specific points in time, be it seed germination, s...

    Authors: Jan F. Humplík, Jakub Dostál, Lydia Ugena, Lukáš Spíchal, Nuria De Diego, Ondřej Vencálek and Tomáš Fürst

    Citation: Plant Methods 2020 16:14

    Content type: Methodology

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  34. Plant root research can provide a way to attain stress-tolerant crops that produce greater yield in a diverse array of conditions. Phenotyping roots in soil is often challenging due to the roots being difficul...

    Authors: Abraham George Smith, Jens Petersen, Raghavendra Selvan and Camilla Ruø Rasmussen

    Citation: Plant Methods 2020 16:13

    Content type: Methodology

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  35. Variable-rate fertilization is crucial in the implementation of precision agriculture and for ensuring reasonable and efficient fertilizer application and nutrient management that is tailored to local conditio...

    Authors: Yinyan Shi, Yang Zhu, Xiaochan Wang, Xin Sun, Yangfen Ding, Wexing Cao and Zhichao Hu

    Citation: Plant Methods 2020 16:11

    Content type: Review

    Published on:

  36. Covalent modifications of histones and histone variants have great influence on chromatin structure, which is involved in the transcriptional regulation of gene expression. Chromatin immunoprecipitation (ChIP)...

    Authors: Xiaorong Huang, Qinwei Pan, Ying Lin, Tingting Gu and Yi Li

    Citation: Plant Methods 2020 16:10

    Content type: Methodology

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  37. Cucurbits produce fruits or vegetables that have great dietary importance and economic significance worldwide. The published genomes of at least 11 cucurbit species are boosting gene mining and novel breeding ...

    Authors: Mei Liu, Zhiling Liang, Miguel A. Aranda, Ni Hong, Liming Liu, Baoshan Kang and Qinsheng Gu

    Citation: Plant Methods 2020 16:9

    Content type: Research

    Published on:

  38. The visualization of internal 3D-structure of tissues at micron resolutions without staining by contrast reagents is desirable in plant researches, and it can be achieved by an X-ray computed tomography (CT) w...

    Authors: Naoki Kunishima, Yoshihiro Takeda, Raita Hirose, Dominika Kalasová, Jakub Šalplachta and Kazuhiko Omote

    Citation: Plant Methods 2020 16:7

    Content type: Methodology

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  39. Root system architecture (RSA) traits are of interest for breeding selection; however, measurement of these traits is difficult, resource intensive, and results in large variability. The advent of computer vis...

    Authors: Kevin G. Falk, Talukder Z. Jubery, Seyed V. Mirnezami, Kyle A. Parmley, Soumik Sarkar, Arti Singh, Baskar Ganapathysubramanian and Asheesh K. Singh

    Citation: Plant Methods 2020 16:5

    Content type: Methodology

    Published on:

  40. The unfolded protein response (UPR) is a highly conserved process in eukaryotic organisms that plays a crucial role in adaptation and development. While the most ubiquitous components of this pathway have been...

    Authors: André Alcântara, Denise Seitner, Fernando Navarrete and Armin Djamei

    Citation: Plant Methods 2020 16:4

    Content type: Methodology

    Published on:

  41. Plant receptors with lysin motifs (LsyM) recognize microbial signals such as fungal chitin and lipo-chitooligosaccharidic Nod factors of nitrogen-fixing rhizobia. It is generally assumed that ligand-induced di...

    Authors: Chun-Lian Li, De-Xing Xue, Yi-Han Wang, Zhi-Ping Xie and Christian Staehelin

    Citation: Plant Methods 2020 16:3

    Content type: Methodology

    Published on:

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