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  1. Low pollen viability may limit grapevine yield under certain conditions, causing relevant economic losses to grape-growers. It is usually evaluated by the quantification of the number of viable and non-viable ...

    Authors: Javier Tello, María Ignacia Montemayor, Astrid Forneck and Javier Ibáñez
    Citation: Plant Methods 2018 14:3
  2. Transient gene expression is a powerful tool to study gene function in plants. In citrus, Agrobacterium transformation is the method of choice for transient expression studies, but this method does not work effic...

    Authors: Amit Levy, Choaa El-Mochtar, Chunxia Wang, Michael Goodin and Vladimir Orbovic
    Citation: Plant Methods 2018 14:2
  3. Plants demonstrate dynamic growth phenotypes that are determined by genetic and environmental factors. Phenotypic analysis of growth features over time is a key approach to understand how plants interact with ...

    Authors: Ji Zhou, Christopher Applegate, Albor Dobon Alonso, Daniel Reynolds, Simon Orford, Michal Mackiewicz, Simon Griffiths, Steven Penfield and Nick Pullen
    Citation: Plant Methods 2017 13:117
  4. Fine-grained recognition of plants from images is a challenging computer vision task, due to the diverse appearance and complex structure of plants, high intra-class variability and small inter-class differenc...

    Authors: Milan Šulc and Jiří Matas
    Citation: Plant Methods 2017 13:115
  5. Developmental biology has made great strides in recent years towards the quantification of cellular properties during development. This requires tissues to be imaged and segmented to generate computerised vers...

    Authors: Annamária Kiss, Typhaine Moreau, Vincent Mirabet, Cerasela Iliana Calugaru, Arezki Boudaoud and Pradeep Das
    Citation: Plant Methods 2017 13:114
  6. Switchgrass (Panicum virgatum), a robust perennial C4-type grass, has been evaluated and designated as a model bioenergy crop by the U.S. DOE and USDA. Conventional breeding of switchgrass biomass is difficult be...

    Authors: Chien-Yuan Lin, Bryon S. Donohoe, Neha Ahuja, Deborah M. Garrity, Rongda Qu, Melvin P. Tucker, Michael E. Himmel and Hui Wei
    Citation: Plant Methods 2017 13:113
  7. To investigate translational regulation of gene expression in plant mitochondria, a mitochondrial polysome isolation protocol was established for tobacco to investigate polysomal mRNA loading as a proxy for tr...

    Authors: Muhammad Waqar Hameed, Ilona Juszczak, Ralph Bock and Joost Thomas van Dongen
    Citation: Plant Methods 2017 13:112
  8. The morphological analysis of olive leaves, fruits and endocarps may represent an efficient tool for the characterization and discrimination of cultivars and the establishment of relationships among them. In r...

    Authors: Konstantinos N. Blazakis, Maria Kosma, George Kostelenos, Luciana Baldoni, Marina Bufacchi and Panagiotis Kalaitzis
    Citation: Plant Methods 2017 13:111
  9. The development of cell pattern in the surface cell layer of the shoot apex can be investigated in vivo by use of a time-lapse confocal images, showing naked meristem in 3D in successive times. However, how th...

    Authors: Krzysztof Kucypera, Marcin Lipowczan, Anna Piekarska-Stachowiak and Jerzy Nakielski
    Citation: Plant Methods 2017 13:110
  10. Sorghum (Sorghum bicolor L.) is one of the world’s most important cereal crops grown for multiple applications and has been identified as a potential biofuel crop. Despite several decades of study, sorghum has be...

    Authors: Srinivas Belide, Thomas Vanhercke, James Robertson Petrie and Surinder Pal Singh
    Citation: Plant Methods 2017 13:109
  11. Plant extracts contain a huge variety of pharmacologically active substances. Conventionally, various chromatographic methods must be applied several times to purify functional compounds to measure their funct...

    Authors: Ye Ji Park, Si Hyun Seong, Min Sun Kim, Sang Wan Seo, Mee Ree Kim and Hyun Sik Kim
    Citation: Plant Methods 2017 13:108
  12. The proportion of granule types in wheat starch is an important characteristic that can affect its functionality. It is widely accepted that granule types are either large, disc-shaped A-type granules or small...

    Authors: Emi Tanaka, Jean-Phillippe F. Ral, Sean Li, Raj Gaire, Colin R. Cavanagh, Brian R. Cullis and Alex Whan
    Citation: Plant Methods 2017 13:107
  13. Several high-throughput molecular genetic analyses rely on high-quality genomic DNA. Copurification of other molecules can negatively impact the functionality of plant DNA preparations employed in these proced...

    Authors: Kelvin T. Chiong, Mona B. Damaj, Carmen S. Padilla, Carlos A. Avila, Shankar R. Pant, Kranthi K. Mandadi, Ninfa R. Ramos, Denise V. Carvalho and T. Erik Mirkov
    Citation: Plant Methods 2017 13:106
  14. Rice panicle phenotyping is important in rice breeding, and rice panicle segmentation is the first and key step for image-based panicle phenotyping. Because of the challenge of illumination differentials, pani...

    Authors: Xiong Xiong, Lingfeng Duan, Lingbo Liu, Haifu Tu, Peng Yang, Dan Wu, Guoxing Chen, Lizhong Xiong, Wanneng Yang and Qian Liu
    Citation: Plant Methods 2017 13:104
  15. Accurately segmenting vegetation from the background within digital images is both a fundamental and a challenging task in phenotyping. The performance of traditional methods is satisfactory in homogeneous env...

    Authors: Pouria Sadeghi-Tehran, Nicolas Virlet, Kasra Sabermanesh and Malcolm J. Hawkesford
    Citation: Plant Methods 2017 13:103
  16. Root systems are highly plastic and adapt according to their soil environment. Studying the particular influence of soils on root development necessitates the adaptation and evaluation of imaging methods for m...

    Authors: Daniel Pflugfelder, Ralf Metzner, Dagmar van Dusschoten, Rüdiger Reichel, Siegfried Jahnke and Robert Koller
    Citation: Plant Methods 2017 13:102
  17. After publication of this article [1], the authors noted the following error. Due to a calculation error in the temperature term f(TL) of the emission standardization algorithm (Eq. 3 of the original paper), the ...

    Authors: Marvin Lüpke, Rainer Steinbrecher, Michael Leuchner and Annette Menzel
    Citation: Plant Methods 2017 13:100

    The original article was published in Plant Methods 2017 13:14

  18. The compressional modulus of elasticity is an important mechanical property for understanding stalk lodging, but this property is rarely available for thin-walled plant stems such as maize and sorghum because ...

    Authors: Loay A. Al-Zube, Daniel J. Robertson, Jean N. Edwards, Wenhuan Sun and Douglas D. Cook
    Citation: Plant Methods 2017 13:99
  19. Plant science uses increasing amounts of phenotypic data to unravel the complex interactions between biological systems and their variable environments. Originally, phenotyping approaches were limited by manua...

    Authors: Benoît Valle, Thierry Simonneau, Romain Boulord, Francis Sourd, Thibault Frisson, Maxime Ryckewaert, Philippe Hamard, Nicolas Brichet, Myriam Dauzat and Angélique Christophe
    Citation: Plant Methods 2017 13:98
  20. Automated species identification is a long term research subject. Contrary to flowers and fruits, leaves are available throughout most of the year. Offering margin and texture to characterize a species, they a...

    Authors: Michael Rzanny, Marco Seeland, Jana Wäldchen and Patrick Mäder
    Citation: Plant Methods 2017 13:97
  21. In maize, silks are hundreds of filaments that simultaneously emerge from the ear for collecting pollen over a period of 1–7 days, which largely determines grain number especially under water deficit. Silk gro...

    Authors: Nicolas Brichet, Christian Fournier, Olivier Turc, Olivier Strauss, Simon Artzet, Christophe Pradal, Claude Welcker, François Tardieu and Llorenç Cabrera-Bosquet
    Citation: Plant Methods 2017 13:96
  22. Improvements in high-throughput phenotyping technologies are rapidly expanding the scope and capacity of plant biology studies to measure growth traits. Nevertheless, the costs of commercial phenotyping equipm...

    Authors: Andrei Dobrescu, Livia C. T. Scorza, Sotirios A. Tsaftaris and Alistair J. McCormick
    Citation: Plant Methods 2017 13:95
  23. Accurate and quantitative phenotypic data in plant breeding programmes is vital in breeding to assess the performance of genotypes and to make selections. Traditional strawberry phenotyping relies on the human...

    Authors: Joe Q. He, Richard J. Harrison and Bo Li
    Citation: Plant Methods 2017 13:93
  24. Wheat is one of the most widely grown crop in temperate climates for food and animal feed. In order to meet the demands of the predicted population increase in an ever-changing climate, wheat production needs ...

    Authors: Aoife Hughes, Karen Askew, Callum P. Scotson, Kevin Williams, Colin Sauze, Fiona Corke, John H. Doonan and Candida Nibau
    Citation: Plant Methods 2017 13:76
  25. Hyperspectral imaging is a technology that can be used to monitor plant responses to stress. Hyperspectral images have a full spectrum for each pixel in the image, 400–2500 nm in this case, giving detailed inf...

    Authors: Dominic Williams, Avril Britten, Susan McCallum, Hamlyn Jones, Matt Aitkenhead, Alison Karley, Ken Loades, Ankush Prashar and Julie Graham
    Citation: Plant Methods 2017 13:74
  26. Flos Chrysanthemi, as a part of Chinese culture for a long history, is valuable for not only environmental decoration but also the medicine and food additive. Due to their voluminously...

    Authors: Heng Zhang, Zhenyi Chen, Taihao Li, Na Chen, Wenjie Xu and Shupeng Liu
    Citation: Plant Methods 2017 13:92
  27. Cultivated strawberry (Fragaria × ananassa Duch.) has homoeologous chromosomes because of allo-octoploidy. For example, two homoeologous chromosomes that belong to different sub-genome of allopolyploids have simi...

    Authors: Tomohiro Yanagi, Kenta Shirasawa, Mayuko Terachi and Sachiko Isobe
    Citation: Plant Methods 2017 13:91
  28. There is currently a high requirement for field phenotyping methodologies/technologies to determine quantitative traits related to crop yield and plant stress responses under field conditions.

    Authors: Jorge Gago, Alisdair R. Fernie, Zoran Nikoloski, Takayuki Tohge, Sebastiá Martorell, José Mariano Escalona, Miquel Ribas-Carbó, Jaume Flexas and Hipólito Medrano
    Citation: Plant Methods 2017 13:90
  29. Cytokinins play many essential roles in plant growth and development, mainly through signal transduction pathways. Although the cytokinin signaling pathway in rice has been clarified, no synthetic reporter for...

    Authors: Jinyuan Tao, Huwei Sun, Pengyuan Gu, Zhihao Liang, Xinni Chen, Jiajing Lou, Guohua Xu and Yali Zhang
    Citation: Plant Methods 2017 13:89
  30. The oomycete Phytophthora infestans causes late blight on potato and tomato. Despite extensive research, the P. infestans-host interaction is still poorly understood. To find new ways to further unravel this inte...

    Authors: Charikleia Schoina, Klaas Bouwmeester and Francine Govers
    Citation: Plant Methods 2017 13:88
  31. Most current methods for constructing guide RNAs (gRNA) for the CRISPR/Cas9 genome editing system, depend on traditional cloning using specific type IIS restriction enzymes and DNA ligation. These methods cons...

    Authors: Aftab A. Khan, Ashraf El-Sayed, Asma Akbar, Arianna Mangravita-Novo, Shaheen Bibi, Zunaira Afzal, David J. Norman and Gul Shad Ali
    Citation: Plant Methods 2017 13:86
  32. Genomic DNA extracted from species of Cactaceae is often contaminated with significant amounts of mucilage and pectin. Pectin is one of the main components of cellular walls, whereas mucilage is a complex poly...

    Authors: César Ramiro Martínez-González, Rosario Ramírez-Mendoza, Jaime Jiménez-Ramírez, Clemente Gallegos-Vázquez and Isolda Luna-Vega
    Citation: Plant Methods 2017 13:82
  33. This review explores how imaging techniques are being developed with a focus on deployment for crop monitoring methods. Imaging applications are discussed in relation to both field and glasshouse-based plants,...

    Authors: Amy Lowe, Nicola Harrison and Andrew P French
    Citation: Plant Methods 2017 13:80
  34. Floral timing is a carefully regulated process, in which the plant determines the optimal moment to switch from the vegetative to reproductive phase. While there are numerous genes known that control flowering...

    Authors: Martijn Fiers, Jorin Hoogenboom, Alice Brunazzi, Tom Wennekes, Gerco C. Angenent and Richard G. H. Immink
    Citation: Plant Methods 2017 13:78
  35. The complex cell wall structure of algae often precludes efficient extraction of their genetic material. The purpose of this study was to design a next-generation sequencing-suitable DNA isolation method for u...

    Authors: Tomasz Jagielski, Jan Gawor, Zofia Bakuła, Karolina Zuchniewicz, Iwona Żak and Robert Gromadka
    Citation: Plant Methods 2017 13:77
  36. Changing the phosphorus (P) nutrition leads to changes in plant metabolism. The aim of this study was to investigate how these changes are reflected in the distribution of 33P and the isotopic composition of oxy...

    Authors: Verena Pfahler, Federica Tamburini, Stefano M. Bernasconi and Emmanuel Frossard
    Citation: Plant Methods 2017 13:75
  37. Phenotyping technologies are expected to provide predictive power for a range of applications in plant and crop sciences. Here, we use the disease pressure of Beet Cyst Nematodes (BCN) on sugar beet as an illu...

    Authors: Samuel Joalland, Claudio Screpanti, Frank Liebisch, Hubert Vincent Varella, Alain Gaume and Achim Walter
    Citation: Plant Methods 2017 13:73
  38. Celiac disease (CD) is caused by specific sequences of gluten proteins found in cereals such as bread wheat (Triticum aestivum ssp. aestivum) and spelt (T. aestivum ssp. spelta). Among them, the α-gliadins displa...

    Authors: Benjamin Dubois, Pierre Bertin, Yordan Muhovski, Emmanuelle Escarnot and Dominique Mingeot
    Citation: Plant Methods 2017 13:72
  39. Seed–soil contact is important to ensure successful germination, however, there is a paucity of reported studies that have quantified the microstructure at and around this critical interface, mainly due to the...

    Authors: Sebastian Blunk, Ali Hafeez Malik, Martine I. de Heer, Tobias Ekblad, Jennifer Bussell, Debbie Sparkes, Kenneth Fredlund, Craig J. Sturrock and Sacha J. Mooney
    Citation: Plant Methods 2017 13:71

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