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  1. To investigate potential effects of herbicide phytotoxic on crops, a major challenge is a lack of non-destructive and rapid methods to detect plant growth that could allow characterization of herbicide-resista...

    Authors: Jian Wang, Chu Zhang, Ying Shi, Meijuan Long, Faisal Islam, Chong Yang, Su Yang, Yong He and Weijun Zhou
    Citation: Plant Methods 2020 16:30
  2. Convolvulus sepium (hedge bindweed) detection in sugar beet fields remains a challenging problem due to variation in appearance of plants, illumination changes, foliage occlusions, and different growth stages und...

    Authors: Junfeng Gao, Andrew P. French, Michael P. Pound, Yong He, Tony P. Pridmore and Jan G. Pieters
    Citation: Plant Methods 2020 16:29
  3. Seed size and number are important plant traits from an ecological and horticultural/agronomic perspective. However, in small-seeded species such as Arabidopsis thaliana, research on seed size and number is limit...

    Authors: Alejandro Morales, J. Teapal, J. M. H. Ammerlaan, X. Yin, J. B. Evers, N. P. R. Anten, R. Sasidharan and M. van Zanten
    Citation: Plant Methods 2020 16:27
  4. Biomacromolecules, the first components of bioactive substances in traditional Chinese medicines (TCM) have wide bioactivity-related efficacy but have not yet been fully appreciated compared to small molecule ...

    Authors: Wen-Jin Zhang, Sheng Wang, Chuan-zhi Kang, Chao-geng Lv, Li Zhou, Lu-Qi Huang and Lan-Ping Guo
    Citation: Plant Methods 2020 16:26
  5. Exogenous application of gamma-aminobutyric acid (GABA) could relieve stress symptoms caused by abiotic stresses including anoxia, heat and cold shock, drought, and salt. However, studying translocation and metab...

    Authors: Faraj Hijaz and Nabil Killiny
    Citation: Plant Methods 2020 16:24
  6. Image processing techniques have been widely used in the analysis of leaf characteristics. Earlier techniques for processing digital RGB color images of plant leaves had several drawbacks, such as inadequate d...

    Authors: Zhengmeng Chen, Fuzheng Wang, Pei Zhang, Chendan Ke, Yan Zhu, Weixing Cao and Haidong Jiang
    Citation: Plant Methods 2020 16:23
  7. Aneuploidy, a condition caused by an imbalance between the relative dosages of chromosomes, generally produces a novel phenotype specific to the molecular karyotype. Few techniques are currently available for ...

    Authors: Guo Wen, Jiangbo Dang, Zhongyi Xie, Jinying Wang, Pengfei Jiang, Qigao Guo and Guolu Liang
    Citation: Plant Methods 2020 16:22
  8. Myxospermy is a process by which the external surfaces of seeds of many plant species produce mucilage—a polysaccharide-rich gel with numerous fundamental research and industrial applications. Due to its funct...

    Authors: James M. Cowley, Lina Herliana, Kylie A. Neumann, Silvano Ciani, Virna Cerne and Rachel A. Burton
    Citation: Plant Methods 2020 16:20
  9. Tea, which is produced from new shoots of existing tea plants (Camellia sinensis), is one of the most popular, non-alcoholic, healthy beverages worldwide. Colletotrichum camelliae is one of the dominant fungal pa...

    Authors: Shengnan He, Huchen Chen, Yi Wei, Tai An and Shouan Liu
    Citation: Plant Methods 2020 16:17
  10. Forward genetic screens are a powerful approach for identifying the genes contributing to a trait of interest. However, mutants arising in genes already known can obscure the identification of new genes contri...

    Authors: Jiangling Cao, Nial R. Gursanscky, Stephen J. Fletcher, Anne Sawyer, Mehershad Wadia, Lachlan McKeough, Marek Coleman, Uwe Dressel, Christelle Taochy, Neena Mitter, Hervé Vaucheret and Bernard J. Carroll
    Citation: Plant Methods 2020 16:16
  11. Improving abiotic stress tolerance in wheat requires large scale screening of yield components such as seed weight, seed number and single seed weight, all of which is very laborious, and a detailed analysis o...

    Authors: Jessica Schmidt, Joelle Claussen, Norbert Wörlein, Anja Eggert, Delphine Fleury, Trevor Garnett and Stefan Gerth
    Citation: Plant Methods 2020 16:15
  12. Plants, like all living organisms, metamorphose their bodies during their lifetime. All the developmental and growth events in a plant’s life are connected to specific points in time, be it seed germination, s...

    Authors: Jan F. Humplík, Jakub Dostál, Lydia Ugena, Lukáš Spíchal, Nuria De Diego, Ondřej Vencálek and Tomáš Fürst
    Citation: Plant Methods 2020 16:14
  13. Plant root research can provide a way to attain stress-tolerant crops that produce greater yield in a diverse array of conditions. Phenotyping roots in soil is often challenging due to the roots being difficul...

    Authors: Abraham George Smith, Jens Petersen, Raghavendra Selvan and Camilla Ruø Rasmussen
    Citation: Plant Methods 2020 16:13
  14. Variable-rate fertilization is crucial in the implementation of precision agriculture and for ensuring reasonable and efficient fertilizer application and nutrient management that is tailored to local conditio...

    Authors: Yinyan Shi, Yang Zhu, Xiaochan Wang, Xin Sun, Yangfen Ding, Wexing Cao and Zhichao Hu
    Citation: Plant Methods 2020 16:11
  15. Covalent modifications of histones and histone variants have great influence on chromatin structure, which is involved in the transcriptional regulation of gene expression. Chromatin immunoprecipitation (ChIP)...

    Authors: Xiaorong Huang, Qinwei Pan, Ying Lin, Tingting Gu and Yi Li
    Citation: Plant Methods 2020 16:10
  16. Cucurbits produce fruits or vegetables that have great dietary importance and economic significance worldwide. The published genomes of at least 11 cucurbit species are boosting gene mining and novel breeding ...

    Authors: Mei Liu, Zhiling Liang, Miguel A. Aranda, Ni Hong, Liming Liu, Baoshan Kang and Qinsheng Gu
    Citation: Plant Methods 2020 16:9
  17. The visualization of internal 3D-structure of tissues at micron resolutions without staining by contrast reagents is desirable in plant researches, and it can be achieved by an X-ray computed tomography (CT) w...

    Authors: Naoki Kunishima, Yoshihiro Takeda, Raita Hirose, Dominika Kalasová, Jakub Šalplachta and Kazuhiko Omote
    Citation: Plant Methods 2020 16:7
  18. Root system architecture (RSA) traits are of interest for breeding selection; however, measurement of these traits is difficult, resource intensive, and results in large variability. The advent of computer vis...

    Authors: Kevin G. Falk, Talukder Z. Jubery, Seyed V. Mirnezami, Kyle A. Parmley, Soumik Sarkar, Arti Singh, Baskar Ganapathysubramanian and Asheesh K. Singh
    Citation: Plant Methods 2020 16:5
  19. The unfolded protein response (UPR) is a highly conserved process in eukaryotic organisms that plays a crucial role in adaptation and development. While the most ubiquitous components of this pathway have been...

    Authors: André Alcântara, Denise Seitner, Fernando Navarrete and Armin Djamei
    Citation: Plant Methods 2020 16:4
  20. Plant receptors with lysin motifs (LsyM) recognize microbial signals such as fungal chitin and lipo-chitooligosaccharidic Nod factors of nitrogen-fixing rhizobia. It is generally assumed that ligand-induced di...

    Authors: Chun-Lian Li, De-Xing Xue, Yi-Han Wang, Zhi-Ping Xie and Christian Staehelin
    Citation: Plant Methods 2020 16:3
  21. Micro-computed tomography (μCT) bring a new opportunity to accurately quantify micro phenotypic traits of maize stem, also provide comparable benchmark to evaluate its dynamic development at the different grow...

    Authors: Ying Zhang, Liming Ma, Jinglu Wang, Xiaodong Wang, Xinyu Guo and Jianjun Du
    Citation: Plant Methods 2020 16:2
  22. Herbaria are valuable sources of extensive curated plant material that are now accessible to genetic studies because of advances in high-throughput, next-generation sequencing methods. As an applied assessment...

    Authors: Paul G. Nevill, Xiao Zhong, Julian Tonti-Filippini, Margaret Byrne, Michael Hislop, Kevin Thiele, Stephen van Leeuwen, Laura M. Boykin and Ian Small
    Citation: Plant Methods 2020 16:1
  23. In the original publication [1], the copyright line was incorrectly published as “© The Author(s) 2019”. The corrected copyright line should read as “© Her Majesty the Queen in Right of Canada as represented b...

    Authors: Xiucheng Cui, Margaret Balcerzak, Johann Schernthaner, Vivijan Babic, Raju Datla, Elizabeth K. Brauer, Natalie Labbé, Rajagopal Subramaniam and Thérèse Ouellet
    Citation: Plant Methods 2019 15:163

    The original article was published in Plant Methods 2019 15:119

  24. Recent advances in image-based plant phenotyping have improved our capability to study vegetative stage growth dynamics. However, more complex agronomic traits such as inflorescence architecture (IA), which pr...

    Authors: Jaspreet Sandhu, Feiyu Zhu, Puneet Paul, Tian Gao, Balpreet K. Dhatt, Yufeng Ge, Paul Staswick, Hongfeng Yu and Harkamal Walia
    Citation: Plant Methods 2019 15:162
  25. High grain breakage rate is the main limiting factor encountered in the mechanical harvest of maize grain. X-ray micro-computed tomography (μCT) scanning technology could be used to obtain the three-dimensiona...

    Authors: Junfeng Hou, Ying Zhang, Xiuliang Jin, Pengfei Dong, Yanan Guo, Keru Wang, Yinghu Fan and Shaokun Li
    Citation: Plant Methods 2019 15:161
  26. Almond is an emerging crop due to the health benefits of almond consumption including nutritional, anti-inflammatory, and hypocholesterolaemia properties. Traditional almond producers were concentrated in Cali...

    Authors: Francisca López-Granados, Jorge Torres-Sánchez, Francisco M. Jiménez-Brenes, Octavio Arquero, María Lovera and Ana I. de Castro
    Citation: Plant Methods 2019 15:160
  27. Seagrass meadows are recognized as critical and among the most vulnerable habitats on the planet. The alarming rates of decline in seagrass meadows have attracted the attention globally. There is an urgent nee...

    Authors: Shidong Yue, Yu Zhang, Yi Zhou, Shaochun Xu, Shuai Xu, Xiaomei Zhang and Ruiting Gu
    Citation: Plant Methods 2019 15:158
  28. The raised demand of cannabis as a medicinal plant in recent years led to an increased interest in understanding the biosynthetic routes of cannabis metabolites. Since there is no established protocol to gener...

    Authors: Julia Schachtsiek, Tajammul Hussain, Khadija Azzouhri, Oliver Kayser and Felix Stehle
    Citation: Plant Methods 2019 15:157
  29. Over the last years, several plant science labs have started to employ fluctuating growth light conditions to simulate natural light regimes more closely. Many plant mutants reveal quantifiable effects under f...

    Authors: Dominik Schneider, Laura S. Lopez, Meng Li, Joseph D. Crawford, Helmut Kirchhoff and Hans-Henning Kunz
    Citation: Plant Methods 2019 15:156
  30. Plant feeding, free-living nematodes cause extensive damage to plant roots by direct feeding and, in the case of some trichodorid and longidorid species, through the transmission of viruses. Developing more en...

    Authors: Felicity E. O’Callaghan, Roy Neilson, Stuart A. MacFarlane and Lionel X. Dupuy
    Citation: Plant Methods 2019 15:155
  31. Guayule (Parthenium argentatum A. Gray), a plant native to semi-arid regions of northern Mexico and southern Texas in the United States, is an alternative source for natural rubber (NR). Rapid screening tools are...

    Authors: Zinan Luo, Kelly R. Thorp and Hussein Abdel-Haleem
    Citation: Plant Methods 2019 15:154
  32. Acoustic emission (AE) sensing is in use since the late 1960s in drought-induced embolism research as a non-invasive and continuous method. It is very well suited to assess a plant’s vulnerability to dehydrati...

    Authors: Niels J. F. De Baerdemaeker, Michiel Stock, Jan Van den Bulcke, Bernard De Baets, Luc Van Hoorebeke and Kathy Steppe
    Citation: Plant Methods 2019 15:153
  33. In the original publication of the article [1], a few errors were identified.

    Authors: Sadiye Hayta, Mark A. Smedley, Selcen U. Demir, Robert Blundell, Alison Hinchliffe, Nicola Atkinson and Wendy A. Harwood
    Citation: Plant Methods 2019 15:152

    The original article was published in Plant Methods 2019 15:121

  34. Plant development is controlled by the action of many, often connected gene regulatory networks. Differential gene expression controlled by internal and external cues is a major driver of growth and time speci...

    Authors: Kimmo Kivivirta, Denise Herbert, Matthias Lange, Knut Beuerlein, Janine Altmüller and Annette Becker
    Citation: Plant Methods 2019 15:151
  35. Deep rooting is one of the most promising plant traits for improving crop yield under water-limited conditions. Most root phenotyping methods are designed for laboratory-grown plants, typically measuring very ...

    Authors: Si Chen, Simon Fiil Svane and Kristian Thorup-Kristensen
    Citation: Plant Methods 2019 15:148
  36. Non-invasive procedures for the diagnosis of viability of plant or fungal tissues would be valuable for scientific, industrial and biomonitoring purposes. Previous studies showed that infrared thermography (IR...

    Authors: Beatriz Fernández-Marín, Othmar Buchner, Gerald Kastberger, Federica Piombino, José Ignacio García-Plazaola and Ilse Kranner
    Citation: Plant Methods 2019 15:147
  37. Potato, the third most important crop worldwide, plays a critical role in human food security. Brown rot, one of the most destructive potato diseases caused by Ralstonia solanacearum, results in huge economic los...

    Authors: Huijuan Wang, Jinxue Hu, Yao Lu, Mancang Zhang, Ning Qin, Ruize Zhang, Yizhe He, Dongdong Wang, Yue Chen, Cuizhu Zhao, Núria S. Coll, Marc Valls, Qin Chen and Haibin Lu
    Citation: Plant Methods 2019 15:145
  38. Insect herbivory poses a major threat to maize. Benzoxazinoids are important anti-insect secondary metabolites in maize, whose biosynthetic pathway has been extensively studied. However, yet little is known ab...

    Authors: Lei Gao, Guojing Shen, Lingdan Zhang, Jinfeng Qi, Cuiping Zhang, Canrong Ma, Jing Li, Lei Wang, Saif Ul Malook and Jianqiang Wu
    Citation: Plant Methods 2019 15:144
  39. Large insert paired-end sequencing technologies are important tools for assembling genomes, delineating associated breakpoints and detecting structural rearrangements. To facilitate the comprehensive detection...

    Authors: Zhaozhao Dai, Tong Li, Jiadong Li, Zhifei Han, Yonglong Pan, Sha Tang, Xianmin Diao and Meizhong Luo
    Citation: Plant Methods 2019 15:142

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