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  1. Some root-parasitic plants belonging to the Orobanche, Phelipanche or Striga genus represent one of the most destructive and intractable weed problems to agricultural production in both developed and developing c...

    Authors: Jean-Bernard Pouvreau, Zachary Gaudin, Bathilde Auger, Marc-Marie Lechat, Mathieu Gauthier, Philippe Delavault and Philippe Simier

    Citation: Plant Methods 2013 9:32

    Content type: Methodology

    Published on:

  2. In this study, a protocol is described for rapid preparation of an enriched, reasonably pure fraction of nuclear proteins from the leaves of tobacco (Nicotiana tabacum), potato (Solanum tuberosum) and apple (Malu...

    Authors: Sidona Sikorskaite, Minna-Liisa Rajamäki, Danas Baniulis, Vidmantas Stanys and Jari PT Valkonen

    Citation: Plant Methods 2013 9:31

    Content type: Methodology

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  3. We present a novel method for quantitative analysis of dicot leaf expansion at high temporal resolution. Image sequences of growing leaves were assessed using a marker tracking algorithm. An important feature ...

    Authors: Michael Mielewczik, Michael Friedli, Norbert Kirchgessner and Achim Walter

    Citation: Plant Methods 2013 9:30

    Content type: Methodology

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  4. With the explosive numbers of sequences generated by next generation sequencing, the demand for high throughput screening to understand gene function has grown. Plant viral vectors have been widely used as too...

    Authors: Huaibi Zhang, Lei Wang, Donald Hunter, Charlotte Voogd, Nigel Joyce and Kevin Davies

    Citation: Plant Methods 2013 9:28

    Content type: Methodology

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  5. Western clover (Trifolium occidentale) is a perennial herb with characteristics compatible for its development as an attractive model species for genomics studies relating to the forage legume, white clover (Trif...

    Authors: Kim A Richardson, Dorothy A Maher, Chris S Jones and Greg Bryan

    Citation: Plant Methods 2013 9:25

    Content type: Methodology

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  6. Wax esters are highly hydrophobic neutral lipids that are major constituents of the cutin and suberin layer. Moreover they have favorable properties as a commodity for industrial applications. Through transgen...

    Authors: Tim Iven, Cornelia Herrfurth, Ellen Hornung, Mareike Heilmann, Per Hofvander, Sten Stymne, Li-Hua Zhu and Ivo Feussner

    Citation: Plant Methods 2013 9:24

    Content type: Methodology

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  7. Advances in research in the past few years on the ornamental plant torenia (Torenia spps.) have made it notable as a model plant on the frontier of genetic engineering aimed at studying ornamental characteristics...

    Authors: Masahiro Nishihara, Takeshi Shimoda, Takashi Nakatsuka and Gen-ichiro Arimura

    Citation: Plant Methods 2013 9:23

    Content type: Review

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  8. Trehalose is a non-reducing disaccharide that is used as an osmolyte, transport sugar, carbon reserve and stress protectant in a wide range of organisms. In plants, trehalose 6-phosphate (Tre6P), the intermedi...

    Authors: Petronia Carillo, Regina Feil, Yves Gibon, Namiko Satoh-Nagasawa, David Jackson, Oliver E Bläsing, Mark Stitt and John Edward Lunn

    Citation: Plant Methods 2013 9:21

    Content type: Methodology

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  9. Nectar reabsorption is a widely known phenomenon, related to the strategy of resource-recovery and also to maintain the nectar homeostasis at the nectary. The method currently performed to demonstrate nectar b...

    Authors: Poliana Cardoso-Gustavson, João Marcelo Robazzi Bignelli Valente Aguiar, Emerson Ricardo Pansarin and Fábio de Barros

    Citation: Plant Methods 2013 9:20

    Content type: Methodology

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  10. When conducting plant research, the measurement of photosynthetic pigments can provide basic information on the physiological status of a plant. High-pressure liquid chromatography (HPLC) is becoming widely us...

    Authors: Xueyun Hu, Ayumi Tanaka and Ryouichi Tanaka

    Citation: Plant Methods 2013 9:19

    Content type: Methodology

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  11. When conducting aphid stylectomy, measuring accurate rates of phloem exudation is difficult because the volumes collected are in the nanolitre (nl) range. In a new method, exudate volume was calculated from op...

    Authors: Lachlan J Palmer, Lyndon T Palmer, Jeremy Pritchard, Robin D Graham and James CR Stangoulis

    Citation: Plant Methods 2013 9:18

    Content type: Methodology

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  12. In order to select for quantitative plant resistance to pathogens, high throughput approaches that can precisely quantify disease severity are needed. Automation and use of calibrated image analysis should pro...

    Authors: Céline Rousseau, Etienne Belin, Edouard Bove, David Rousseau, Frédéric Fabre, Romain Berruyer, Jacky Guillaumès, Charles Manceau, Marie-Agnès Jacques and Tristan Boureau

    Citation: Plant Methods 2013 9:17

    Content type: Methodology

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  13. Plant extracts are a reservoir of pharmacologically active substances; however, conventional analytical methods can analyze only a small portion of an extract. Here, we report a high-throughput analytical meth...

    Authors: Kyu Hwan Park, Min Sun Kim, Sun Jong Baek, Ik Hyun Bae, Sang-Wan Seo, Jongjin Kim, Yong Kook Shin, Yong-Moon Lee and Hyun Sik Kim

    Citation: Plant Methods 2013 9:15

    Content type: Methodology

    Published on:

  14. Grafting procedures are an excellent tool to study long range signalling processes within a plant. In the last decade, suitable flat-surface grafting procedures for young Arabidopsis seedlings using a collar t...

    Authors: Nayelli Marsch-Martínez, John Franken, Karla L Gonzalez-Aguilera, Stefan de Folter, Gerco Angenent and Elena R Alvarez-Buylla

    Citation: Plant Methods 2013 9:14

    Content type: Methodology

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  15. Brassinosteriods (BRs), a group of important phytohormones, have various effects on plant growth and development. However, their physiological functions in plants have not been fully understood to date. Endoge...

    Authors: Jun Ding, Li-Jing Mao, Bi-Feng Yuan and Yu-Qi Feng

    Citation: Plant Methods 2013 9:13

    Content type: Methodology

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  16. The hydraulic conductivity of the stem is a major factor limiting the capability of trees to transport water from the soil to transpiring leaves. During drought conditions, the conducting capacity of xylem can...

    Authors: Jussi-Petteri Suuronen, Marko Peura, Kurt Fagerstedt and Ritva Serimaa

    Citation: Plant Methods 2013 9:11

    Content type: Methodology

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  17. X-ray micro-Computed Tomography (μCT) offers the ability to visualise the three-dimensional structure of plant roots growing in their natural environment – soil. Recovery of root architecture descriptions from...

    Authors: Stefan Mairhofer, Susan Zappala, Saoirse Tracy, Craig Sturrock, Malcolm John Bennett, Sacha Jon Mooney and Tony Paul Pridmore

    Citation: Plant Methods 2013 9:8

    Content type: Software

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  18. Heat stress and heat damage to plants gain globally increasing importance for crop production and plant survival in endangered habitats. Therefore the knowledge of heat tolerance of plants is of great interest...

    Authors: Othmar Buchner, Matthias Karadar, Ines Bauer and Gilbert Neuner

    Citation: Plant Methods 2013 9:7

    Content type: Methodology

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  19. Public genomic databases have provided new directions for molecular marker development and initiated a shift in the types of PCR-based techniques commonly used in plant science. Alongside commonly used arbitra...

    Authors: Péter Poczai, Ildikó Varga, Maarja Laos, András Cseh, Neil Bell, Jari PT Valkonen and Jaakko Hyvönen

    Citation: Plant Methods 2013 9:6

    Content type: Review

    Published on:

  20. There are a number of unresolved issues in the design of experiments in greenhouses. They include whether statistical designs should be used and, if so, which designs should be used. Also, are there thigmomorp...

    Authors: Chris J Brien, Bettina Berger, Huwaida Rabie and Mark Tester

    Citation: Plant Methods 2013 9:5

    Content type: Methodology

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  21. Hydroponic growth systems are a convenient platform for studying whole plant physiology. However, we found through trialling systems as they are described in the literature that our experiments were frequently...

    Authors: Simon J Conn, Bradleigh Hocking, Maclin Dayod, Bo Xu, Asmini Athman, Sam Henderson, Lucy Aukett, Vanessa Conn, Monique K Shearer, Sigfredo Fuentes, Stephen D Tyerman and Matthew Gilliham

    Citation: Plant Methods 2013 9:4

    Content type: Methodology

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  22. Rhizo-lysimeters offer unique advantages for the study of plants and their interactions with soils. In this paper, an existing facility at Charles Sturt University in Wagga Wagga Australia is described in deta...

    Authors: Philip L Eberbach, Jeffrey Hoffmann, Sergio J Moroni, Leonard J Wade and Leslie A Weston

    Citation: Plant Methods 2013 9:3

    Content type: Methodology

    Published on:

  23. Root growth is highly responsive to temporal changes in the environment. On the contrary, diel (24 h) leaf expansion in dicot plants is governed by endogenous control and therefore its temporal pattern does no...

    Authors: Tom Ruts, Shizue Matsubara and Achim Walter

    Citation: Plant Methods 2013 9:2

    Content type: Methodology

    Published on:

  24. Plant grafting techniques have deepened our understanding of the signals facilitating communication between the root and shoot, as well as between shoot and reproductive organs. Transmissible signalling molecu...

    Authors: Nazia Nisar, Shelley Verma, Barry J Pogson and Christopher I Cazzonelli

    Citation: Plant Methods 2012 8:50

    Content type: Methodology

    Published on:

  25. Quantification of callose deposits is a useful measure for the activities of plant immunity and pathogen growth by fluorescence imaging. For robust scoring of differences, this normally requires many technical...

    Authors: Ji Zhou, Thomas Spallek, Christine Faulkner and Silke Robatzek

    Citation: Plant Methods 2012 8:49

    Content type: Methodology

    Published on:

  26. In this report we describe a chromatin immunoprecipitation (ChIP) protocol for two fully sequenced model diatom species Phaeodactylum tricornutum and Thalassiosira pseudonana. This protocol allows the extraction ...

    Authors: Xin Lin, Leïla Tirichine and Chris Bowler

    Citation: Plant Methods 2012 8:48

    Content type: Methodology

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  27. Phytohormones are the key metabolites participating in the regulation of multiple functions of plant organism. Among them, jasmonates, as well as abscisic and salicylic acids are responsible for triggering and...

    Authors: Gerd Ulrich Balcke, Vinzenz Handrick, Nick Bergau, Mandy Fichtner, Anja Henning, Hagen Stellmach, Alain Tissier, Bettina Hause and Andrej Frolov

    Citation: Plant Methods 2012 8:47

    Content type: Methodology

    Published on:

  28. Accurate characterization of complex plant phenotypes is critical to assigning biological functions to genes through forward or reverse genetics. It can also be vital in determining the effect of a treatment, ...

    Authors: Jason M Green, Heidi Appel, Erin MacNeal Rehrig, Jaturon Harnsomburana, Jia-Fu Chang, Peter Balint-Kurti and Chi-Ren Shyu

    Citation: Plant Methods 2012 8:45

    Content type: Methodology

    Published on:

  29. The male germline in flowering plants differentiates by asymmetric division of haploid uninucleated microspores, giving rise to a vegetative cell enclosing a smaller generative cell, which eventually undergoes...

    Authors: Filipe Borges, Rui Gardner, Telma Lopes, Joseph P Calarco, Leonor C Boavida, R Keith Slotkin, Robert A Martienssen and Jörg D Becker

    Citation: Plant Methods 2012 8:44

    Content type: Methodology

    Published on:

  30. A large number of different plant lines are produced and maintained in a typical plant research laboratory, both as seed stocks and in active growth. These collections need careful and consistent management to...

    Authors: Jeroen Nieuwland, Emily Sornay, Angela Marchbank, Barend HJ de Graaf and James AH Murray

    Citation: Plant Methods 2012 8:43

    Content type: Software

    Published on:

  31. Cytokinins (CKs) are a group of plant growth regulators that are involved in several plant developmental processes. Despite the breadth of knowledge surrounding CKs and their diverse functions, much remains to...

    Authors: Scott C Farrow and RJ Neil Emery

    Citation: Plant Methods 2012 8:42

    Content type: Methodology

    Published on:

  32. Deep sequencing is a powerful tool for novel small RNA discovery. Illumina small RNA sequencing library preparation requires a pre-adenylated 3’ end adapter containing a 5’,5’-adenyl pyrophosphoryl moiety. In ...

    Authors: Yun-Ru Chen, Yi Zheng, Bao Liu, Silin Zhong, Jim Giovannoni and Zhangjun Fei

    Citation: Plant Methods 2012 8:41

    Content type: Methodology

    Published on:

  33. Some prominent cultured plant cell lines, such as the BY-2 cell line of tobacco (Nicotiana tabacum cv. ‘Bright Yellow 2’) and the T87 cell line of Arabidopsis (Arabidopsis thaliana L. Heynh., ecotype Columbia) ar...

    Authors: Shinjiro Ogita, Taiji Nomura, Takao Kishimoto and Yasuo Kato

    Citation: Plant Methods 2012 8:40

    Content type: Methodology

    Published on:

  34. Phytophthora cinnamomi Rands. is an important root rot pathogen widely distributed in the north hemisphere, with a large host range. Among others diseases, it is known to be a principal factor in the decline of h...

    Authors: Francisco J Ruiz-Gómez, Rafael Sánchez-Cuesta, Rafael M Navarro-Cerrillo and Alejandro Pérez-de-Luque

    Citation: Plant Methods 2012 8:39

    Content type: Methodology

    Published on:

  35. Long distance signaling is a common phenomenon in animal and plant development. In plants, lateral organs such as nodules and lateral roots are developmentally regulated by root-to-shoot and shoot-to-root long...

    Authors: Tessema K Kassaw and Julia A Frugoli

    Citation: Plant Methods 2012 8:38

    Content type: Methodology

    Published on:

  36. Starch is the most important source of calories for human nutrition and the majority of it is produced by cereal farming. Starch is also used as a renewable raw material in a range of industrial sectors. It ca...

    Authors: Massimiliano Carciofi, Andreas Blennow, Morten M Nielsen, Preben B Holm and Kim H Hebelstrup

    Citation: Plant Methods 2012 8:36

    Content type: Methodology

    Published on:

  37. Although Single Nucleotide Polymorphism (SNP) marker is an invaluable tool for positional cloning, association study and evolutionary analysis, low SNP detection efficiency by Allele-Specific PCR (AS-PCR) stil...

    Authors: Jing Liu, Shunmou Huang, Meiyu Sun, Shengyi Liu, Yumei Liu, Wanxing Wang, Xiurong Zhang, Hanzhong Wang and Wei Hua

    Citation: Plant Methods 2012 8:34

    Content type: Methodology

    Published on:

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