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  1. The creation of minimally redundant tile paths (hereafter MTP) from contiguous sets of overlapping clones (hereafter contigs) in physical maps is a critical step for structural and functional genomics. Build 4...

    Authors: JL Shultz, C Yesudas, S Yaegashi, AJ Afzal, S Kazi and DA Lightfoot

    Citation: Plant Methods 2006 2:9

    Content type: Methodology

    Published on:

  2. Uncovering the key sequence elements in gene promoters that regulate the expression of plant genomes is a huge task that will require a series of complementary methods for prediction, substantial innovations i...

    Authors: Kathryn E Holt, A Harvey Millar and James Whelan

    Citation: Plant Methods 2006 2:8

    Content type: Methodology

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  3. The pH is an important parameter controlling many metabolic and signalling pathways in living cells. Recombinant fluorescent pH indicators (pHluorins) have come into vogue for monitoring cellular pH. They are ...

    Authors: Alexander Schulte, Inken Lorenzen, Markus Böttcher and Christoph Plieth

    Citation: Plant Methods 2006 2:7

    Content type: Methodology

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  4. As an Open Access journal dedicated to promoting technological innovation in plant biology, Plant Methods occupies a unique niche amongst plant journals. To fund its open access policy, and to enable it to contin...

    Authors: Brian G Forde and Michael R Roberts

    Citation: Plant Methods 2006 2:6

    Content type: Editorial

    Published on:

  5. In order to understand microarray data reasonably in the context of other existing biological knowledge, it is necessary to conduct a thorough examination of the data utilizing every aspect of available omic k...

    Authors: Yoshikazu Hasegawa, Motoaki Seki, Yoshiki Mochizuki, Naohiko Heida, Katsura Hirosawa, Naoki Okamoto, Tetsuya Sakurai, Masakazu Satou, Kenji Akiyama, Kei Iida, Kisik Lee, Shigehiko Kanaya, Taku Demura, Kazuo Shinozaki, Akihiko Konagaya and Tetsuro Toyoda

    Citation: Plant Methods 2006 2:5

    Content type: Methodology

    Published on:

  6. The preparation of expressional cDNA libraries for use in the yeast two-hybrid system is quick and efficient when using the dedicated Clontech™ product, the MATCHMAKER Library Construction and Screening Kit 3....

    Authors: Sergiy Lopato, Natalia Bazanova, Sarah Morran, Andrew S Milligan, Neil Shirley and Peter Langridge

    Citation: Plant Methods 2006 2:3

    Content type: Methodology

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  7. Abscission is the regulated dropping of plant organs, such as leaves or flower petals. This process involves a break down of the cell wall between layers of cells in the abscission zone, causing the organ to b...

    Authors: Kevin A Lease, Sung Ki Cho and John C Walker

    Citation: Plant Methods 2006 2:2

    Content type: Methodology

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  8. Appropriate biological interpretation of microarray data calls for relevant experimental annotation. The widely accepted MIAME guidelines provide a generic, organism-independant standard for minimal informatio...

    Authors: Philip Zimmermann, Beatrice Schildknecht, David Craigon, Margarita Garcia-Hernandez, Wilhelm Gruissem, Sean May, Gaurab Mukherjee, Helen Parkinson, Seung Rhee, Ulrich Wagner and Lars Hennig

    Citation: Plant Methods 2006 2:1

    Content type: Commentary

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  9. In silico analyses based on sequence similarities with animal channels have identified a large number of plant genes likely to encode ion channels. The attempts made to characterise such putative plant channel...

    Authors: E Hosy, G Duby, A-A Véry, A Costa, H Sentenac and J-B Thibaud

    Citation: Plant Methods 2005 1:14

    Content type: Methodology

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  10. We describe novel plasmid vectors for transient gene expression using Agrobacterium, infiltrated into Nicotiana benthamiana leaves. We have generated a series of pGreenII cloning vectors that are ideally suited t...

    Authors: Roger P Hellens, Andrew C Allan, Ellen N Friel, Karen Bolitho, Karryn Grafton, Matthew D Templeton, Sakuntala Karunairetnam, Andrew P Gleave and William A Laing

    Citation: Plant Methods 2005 1:13

    Content type: Research

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  11. High-density oligonucleotide (oligo) arrays are a powerful tool for transcript profiling. Arrays based on GeneChip® technology are amongst the most widely used, although GeneChip® arrays are currently available f...

    Authors: John P Hammond, Martin R Broadley, David J Craigon, Janet Higgins, Zoe F Emmerson, Henrik J Townsend, Philip J White and Sean T May

    Citation: Plant Methods 2005 1:10

    Content type: Methodology

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  12. Protein phosphorylation is accepted as a major regulatory pathway in plants. More than 1000 protein kinases are predicted in the Arabidopsis proteome, however, only a few studies look systematically for in vivo p...

    Authors: Florian Wolschin and Wolfram Weckwerth

    Citation: Plant Methods 2005 1:9

    Content type: Methodology

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  13. In situ hybridisation can provide cellular, and in some cases sub-cellular, resolution of mRNA levels within multicellular organisms and is widely used to provide spatial and temporal information on gene expressi...

    Authors: Sinéad Drea, Julia Corsar, Brian Crawford, Peter Shaw, Liam Dolan and John H Doonan

    Citation: Plant Methods 2005 1:8

    Content type: Methodology

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  14. Eukaryotic organisms are defined by the presence of a nucleus, which encloses the chromosomal DNA, and is characterized by its DNA content (C-value). Complex eukaryotic organisms contain organs and tissues tha...

    Authors: Changqing Zhang, Fang Cheng Gong, Georgina M Lambert and David W Galbraith

    Citation: Plant Methods 2005 1:7

    Content type: Methodology

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  15. Plant genome sequence data now provide opportunities to conduct molecular genetic studies at the level of the whole gene locus and above. Such studies will be greatly facilitated by adopting and developing fur...

    Authors: Laura C Roden, Berthold Göttgens and Effie S Mutasa-Göttgens

    Citation: Plant Methods 2005 1:6

    Content type: Methodology

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  16. Many established PCR-based approaches in plant molecular biology rely on lengthy and expensive methods for isolation of nucleic acids. Although several rapid DNA isolation protocols are available, they have no...

    Authors: Kenneth Berendzen, Iain Searle, Dean Ravenscroft, Csaba Koncz, Alfred Batschauer, George Coupland, Imre E Somssich and Bekir Ülker

    Citation: Plant Methods 2005 1:4

    Content type: Methodology

    Published on:

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