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  1. Content type: Methodology

    Progress in plant cell cycle research is highly dependent on reliable methods for detection of cells replicating DNA. Frequency of S-phase cells (cells in DNA synthesis phase) is a basic parameter in studies o...

    Authors: Edit Kotogány, Dénes Dudits, Gábor V Horváth and Ferhan Ayaydin

    Citation: Plant Methods 2010 6:5

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  2. Content type: Methodology

    TILLING (Targeting Induced Local Lesions in Genomes) is a reverse genetics procedure for identifying point mutations in selected gene(s) amplified from a mutagenized population using high-throughput detection ...

    Authors: Yellamaraju Sreelakshmi, Soni Gupta, Reddaiah Bodanapu, Vineeta Singh Chauhan, Mickey Hanjabam, Sherinmol Thomas, Vijee Mohan, Sulabha Sharma, Rajeswari Srinivasan and Rameshwar Sharma

    Citation: Plant Methods 2010 6:3

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  3. Content type: Methodology

    Interphase chromosome organization and dynamics can be studied in living cells using fluorescent tagging techniques that exploit bacterial operator/repressor systems and auto-fluorescent proteins. A nuclear-lo...

    Authors: Antonius JM Matzke, Koichi Watanabe, Johannes van der Winden, Ulf Naumann and Marjori Matzke

    Citation: Plant Methods 2010 6:2

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  4. Content type: Methodology

    The β-glucuronidase (GUS) gene reporter system is one of the most effective and employed techniques in the study of gene regulation in plant molecular biology. Improving protocols for GUS assays have rendered ...

    Authors: Simone Fior and Paolo D Gerola

    Citation: Plant Methods 2009 5:19

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  5. Content type: Software

    The data generated during a course of a biological experiment/study can be sometimes be massive and its management becomes quite critical for the success of the investigation undertaken. The accumulation and a...

    Authors: Raju Naik Vankadavath, Appibhai Jakir Hussain, Reddaiah Bodanapu, Eros Kharshiing, Pinjari Osman Basha, Soni Gupta, Yellamaraju Sreelakshmi and Rameshwar Sharma

    Citation: Plant Methods 2009 5:18

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  6. Content type: Methodology

    Aliphatic molecules containing free carboxyl groups are important intermediates in many metabolic and signalling reactions, however, they accumulate to low levels in tissues and are not efficiently ionized by ...

    Authors: Mario Kallenbach, Ian T Baldwin and Gustavo Bonaventure

    Citation: Plant Methods 2009 5:17

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  7. Content type: Methodology

    Protoplasts isolated from leaves are useful materials in plant research. One application, the transient expression of recombinant genes using Arabidopsis mesophyll protoplasts (TEAMP), is currently commonly used ...

    Authors: Fu-Hui Wu, Shu-Chen Shen, Lan-Ying Lee, Shu-Hong Lee, Ming-Tsar Chan and Choun-Sea Lin

    Citation: Plant Methods 2009 5:16

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  8. Content type: Methodology

    The diploid woodland strawberry (Fragaria vesca) is an attractive system for functional genomics studies. Its small stature, fast regeneration time, efficient transformability and small genome size, together with...

    Authors: Janet P Slovin, Kyle Schmitt and Kevin M Folta

    Citation: Plant Methods 2009 5:15

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  9. Content type: Methodology

    The progress and completion of various plant genome sequencing projects has paved the way for diverse functional genomic studies that involve cloning, modification and subsequent expression of target genes. Th...

    Authors: Ivan I Atanassov, Ilian I Atanassov, JPeter Etchells and Simon R Turner

    Citation: Plant Methods 2009 5:14

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  10. Content type: Methodology

    The establishment of mutant populations together with the strategies for targeted mutation detection has been applied successfully to a large number of organisms including many species in the plant kingdom. Co...

    Authors: Antoine LF Gady, Freddy WK Hermans, Marion HBJ Van de Wal, Eibertus N van Loo, Richard GF Visser and Christian WB Bachem

    Citation: Plant Methods 2009 5:13

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  11. Content type: Methodology

    Quantitative multi-elemental analysis by inductively coupled plasma (ICP) spectrometry depends on a complete digestion of solid samples. However, fast and thorough sample digestion is a challenging analytical ...

    Authors: Thomas H Hansen, Kristian H Laursen, Daniel P Persson, Pai Pedas, Søren Husted and Jan K Schjoerring

    Citation: Plant Methods 2009 5:12

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  12. Content type: Methodology

    Fluorescent hybridization techniques are widely used to study the functional organization of different compartments within the mammalian nucleus. However, few examples of such studies are known in the plant ki...

    Authors: Leïla Tirichine, Philippe Andrey, Eric Biot, Yves Maurin and Valérie Gaudin

    Citation: Plant Methods 2009 5:11

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  13. Content type: Methodology

    Dense genetic maps, together with the efficiency and accuracy of their construction, are integral to genetic studies and marker assisted selection for plant breeding. High-throughput multiplex markers that are...

    Authors: Maggie Knox, Carol Moreau, James Lipscombe, David Baker and Noel Ellis

    Citation: Plant Methods 2009 5:10

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  14. Content type: Methodology

    Gene silencing is proving to be a powerful tool for genetic, developmental, and physiological analyses. The use of viral induced gene silencing (VIGS) offers advantages to transgenic approaches as it can be po...

    Authors: Edward M Golenberg, DNoah Sather, Leandria C Hancock, Kenneth J Buckley, Natalie M Villafranco and David M Bisaro

    Citation: Plant Methods 2009 5:9

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  15. Content type: Methodology

    Plant genome sequencing has resulted in the identification of a large number of uncharacterized genes. To investigate these unknown gene functions, several transient transformation systems have been developed ...

    Authors: Jian-Feng Li, Eunsook Park, Albrecht G von Arnim and Andreas Nebenführ

    Citation: Plant Methods 2009 5:6

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  16. Content type: Research

    The concept of metabolite profiling has been around for decades and technical innovations are now enabling it to be carried out on a large scale with respect to the number of both metabolites measured and expe...

    Authors: Anna Lytovchenko, Romina Beleggia, Nicolas Schauer, Tal Isaacson, Jan E Leuendorf, Hanjo Hellmann, Jocelyn KC Rose and Alisdair R Fernie

    Citation: Plant Methods 2009 5:4

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  17. Content type: Methodology

    Generating and identifying transformants is essential for many studies of gene function. In Arabidopsis thaliana, a revolutionary protocol termed floral dip is now the most widely used transformation method. Alth...

    Authors: Amanda M Davis, Anthony Hall, Andrew J Millar, Chiarina Darrah and Seth J Davis

    Citation: Plant Methods 2009 5:3

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  18. Content type: Methodology

    A plethora of concurrent cellular activities is mobilised in the adaptation of plants to adverse environmental conditions. This response can be quantified by physiological experiments or metabolic profiling. T...

    Authors: Livia Saleh and Christoph Plieth

    Citation: Plant Methods 2009 5:2

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  19. Content type: Methodology

    The investigation of protein-protein interactions is important for characterizing protein function. Bimolecular fluorescence complementation (BiFC) has recently gained interest as a relatively easy and inexpen...

    Authors: Lan-Ying Lee, Mei-Jane Fang, Lin-Yun Kuang and Stanton B Gelvin

    Citation: Plant Methods 2008 4:24

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  20. Content type: Methodology

    Plant transformation is an invaluable tool for basic plant research, as well as a useful technique for the direct improvement of commercial crops. Barley (Hordeum vulgare) is the fourth most abundant cereal crop ...

    Authors: Joanne G Bartlett, Sílvia C Alves, Mark Smedley, John W Snape and Wendy A Harwood

    Citation: Plant Methods 2008 4:22

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  21. Content type: Methodology

    The actin cytoskeleton responds quickly to diverse stimuli and plays numerous roles in cellular signalling, organelle motility and subcellular compartmentation during plant growth and development. Molecular an...

    Authors: Mike Schenkel, Alison M Sinclair, Daniel Johnstone, JDerek Bewley and Jaideep Mathur

    Citation: Plant Methods 2008 4:21

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  22. Content type: Methodology

    Allotetraploid white clover (Trifolium repens L.) is an important forage legume widely cultivated in most temperate regions. Only a small number of microsatellite markers are publicly available and can be utilize...

    Authors: Yan Zhang, Ji He, Patrick X Zhao, Joseph H Bouton and Maria J Monteros

    Citation: Plant Methods 2008 4:19

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  23. Content type: Methodology

    Medicago truncatula is a model legume species that is currently the focus of an international genome sequencing effort. Although several different oligonucleotide and cDNA arrays have been produced for genome-wid...

    Authors: Klementina Kakar, Maren Wandrey, Tomasz Czechowski, Tanja Gaertner, Wolf-Rüdiger Scheible, Mark Stitt, Ivone Torres-Jerez, Yongli Xiao, Julia C Redman, Hank C Wu, Foo Cheung, Christopher D Town and Michael K Udvardi

    Citation: Plant Methods 2008 4:18

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  24. Content type: Research

    Transcription factors (TFs) co-ordinately regulate target genes that are dispersed throughout the genome. This co-ordinate regulation is achieved, in part, through the interaction of transcription factors with...

    Authors: Andrew P Dare, Robert J Schaffer, Kui Lin-Wang, Andrew C Allan and Roger P Hellens

    Citation: Plant Methods 2008 4:17

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  25. Content type: Methodology

    We describe an efficient method for the rapid quantitative determination of the abundance of three acidic plant hormones from a single crude extract directly by LC/MS/MS. The method exploits the sensitivity of...

    Authors: Silvia Forcat, Mark H Bennett, John W Mansfield and Murray R Grant

    Citation: Plant Methods 2008 4:16

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  26. Content type: Methodology

    Quantitative Real Time RT-PCR (q2(RT)PCR) is a maturing technique which gives researchers the ability to quantify and compare very small amounts of nucleic acids. Primer design and optimization is an essential...

    Authors: Richard M Sharpe, Sade N Dunn and A Bruce Cahoon

    Citation: Plant Methods 2008 4:14

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  27. Content type: Methodology

    We report the development of a microarray platform for rapid and cost-effective genetic mapping, and its evaluation using rice as a model. In contrast to methods employing whole-genome tiling microarrays for g...

    Authors: Jeremy D Edwards, Jaroslav Janda, Megan T Sweeney, Ambika B Gaikwad, Bin Liu, Hei Leung and David W Galbraith

    Citation: Plant Methods 2008 4:13

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  28. Content type: Methodology

    For omics experiments, detailed characterisation of experimental material with respect to its genetic features, its cultivation history and its treatment history is a requirement for analyses by bioinformatics...

    Authors: Karin I Köhl, Georg Basler, Alexander Lüdemann, Joachim Selbig and Dirk Walther

    Citation: Plant Methods 2008 4:11

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  29. Content type: Commentary

    The genus Brassica (Brassicaceae, Brassiceae) is closely related to the model plant Arabidopsis, and includes several important crop plants. Against the background of ongoing genome sequencing, and in line with e...

    Authors: Lars Østergaard and Graham J King

    Citation: Plant Methods 2008 4:10

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  30. Content type: Research

    The isolation of green fluorescent protein (GFP) and the development of spectral variants over the past decade have begun to reveal the dynamic nature of protein trafficking and organelle motility. In planta anal...

    Authors: Naohiro Kato, Dexter Reynolds, Matthew L Brown, Marietta Boisdore, Yukichi Fujikawa, Andrea Morales and Lee A Meisel

    Citation: Plant Methods 2008 4:9

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  31. Content type: Methodology

    Microsatellites are popular molecular markers in many plant species due to their stable and highly polymorphic nature. A number of analysis methods have been described but analyses of these markers are typical...

    Authors: John F Mackay, Christopher D Wright and Roderick G Bonfiglioli

    Citation: Plant Methods 2008 4:8

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  32. Content type: Methodology

    A common limitation in guard cell signaling research is that it is difficult to obtain consistent high expression of transgenes of interest in Arabidopsis guard cells using known guard cell promoters or the const...

    Authors: Yingzhen Yang, Alex Costa, Nathalie Leonhardt, Robert S Siegel and Julian I Schroeder

    Citation: Plant Methods 2008 4:6

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