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  1. Large-scale genetic screens in Arabidopsis are a powerful approach for molecular dissection of complex signaling networks. However, map-based cloning can be time-consuming or even hampered due to low chromosomal ...

    Authors: Kun-hsiang Liu, Matthew McCormack and Jen Sheen

    Citation: Plant Methods 2012 8:12

    Content type: Methodology

    Published on:

  2. Accurate quantification of xylem sap ABA concentrations is important to underpin models of root-to-shoot ABA signalling to predict the physiological effects of soil drying. Growing tomato plants in a whole pla...

    Authors: Andrew G Netting, Julian C Theobald and Ian C Dodd

    Citation: Plant Methods 2012 8:11

    Content type: Methodology

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  3. Cytosine methylation is involved in epigenetic control of gene expression in a wide range of organisms. An increasing number of examples indicate that changing the frequency of cytosine methylation in the geno...

    Authors: Sachiko Arase, Megumi Kasai and Akira Kanazawa

    Citation: Plant Methods 2012 8:185

    Content type: Research

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    The Erratum to this article has been published in Plant Methods 2015 11:21

  4. The ability to quantify the geometry of plant organs at the cellular scale can provide novel insights into their structural organization. Hitherto manual methods of measurement provide only very low throughput...

    Authors: Andrew P French, Michael H Wilson, Kim Kenobi, Daniela Dietrich, Ute Voß, Susana Ubeda-Tomás, Tony P Pridmore and Darren M Wells

    Citation: Plant Methods 2012 8:7

    Content type: Software

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  5. Arabidopsis NPR1 is a master regulator of systemic acquired resistance. NPR1 binds to TGA transcription factors and functions as a transcriptional co-activator. In rice, NH1/OsNPR1 functions to enhance innate ...

    Authors: Mawsheng Chern, Wei Bai, Wing Hoi Sze-To, Patrick E Canlas, Laura E Bartley and Pamela C Ronald

    Citation: Plant Methods 2012 8:6

    Content type: Research

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  6. As a result of the growing body of protein phosphorylation sites data, the number of phosphoprotein databases is constantly increasing, and dozens of tools are available for predicting protein phosphorylation ...

    Authors: Shufu Que, Kuan Li, Min Chen, Yongfei Wang, Qiaobin Yang, Wenfeng Zhang, Baoqian Zhang, Bangshu Xiong and Huaqin He

    Citation: Plant Methods 2012 8:5

    Content type: Methodology

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  7. The repeated weekly subculture of plant cell suspension is labour intensive and increases the risk of variation from parental cells lines. Most of the procedures to preserve cultures are based on controlled fr...

    Authors: Anne-Marie Boisson, Elisabeth Gout, Richard Bligny and Corinne Rivasseau

    Citation: Plant Methods 2012 8:4

    Content type: Methodology

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  8. Hyperspectral imaging (HSI) offers high potential as a non-invasive diagnostic tool for disease detection. In this paper leaf characteristics and spectral reflectance of sugar beet leaves diseased with Cercospora

    Authors: Anne-Katrin Mahlein, Ulrike Steiner, Christian Hillnhütter, Heinz-Wilhelm Dehne and Erich-Christian Oerke

    Citation: Plant Methods 2012 8:3

    Content type: Methodology

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  9. Simultaneous analysis of multiple functional-related phytohormones and their metabolites will improve our understanding of interactions among different hormones in the same biologic process.

    Authors: Hongbo Liu, Xianghua Li, Jinghua Xiao and Shiping Wang

    Citation: Plant Methods 2012 8:2

    Content type: Methodology

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  10. Carotenoids are the most widespread group of pigments found in nature. In addition to their role in the physiology of the plant, carotenoids also have nutritional relevance as their incorporation in the human ...

    Authors: Djédoux Maxime Angaman, Rocco Petrizzo, Francesc Hernández-Gras, Carmen Romero-Segura, Irene Pateraki, Montserrat Busquets and Albert Boronat

    Citation: Plant Methods 2012 8:1

    Content type: Methodology

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  11. Rice genome sequencing projects have generated remarkable amount of information about genes and genome architecture having tremendous potential to be utilized in both basic and applied research. Success in tra...

    Authors: Khirod K Sahoo, Amit K Tripathi, Ashwani Pareek, Sudhir K Sopory and Sneh L Singla-Pareek

    Citation: Plant Methods 2011 7:49

    Content type: Methodology

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  12. Proteins in the plant apoplast are essential for many physiological processes. We have analysed and compared six different infiltration solutions for proteins contained in the apoplast to recognize the most su...

    Authors: Katja Witzel, Muhammad Shahzad, Andrea Matros, Hans-Peter Mock and Karl H Mühling

    Citation: Plant Methods 2011 7:48

    Content type: Methodology

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  13. Pea has lagged behind other model legumes in the molecular study of nodulation and mycorrhizae-formation because of the difficulty to transform its roots and its poor growth on agar plates. Here we describe fo...

    Authors: Scott R Clemow, Lindsey Clairmont, Lene H Madsen and Frédérique C Guinel

    Citation: Plant Methods 2011 7:46

    Content type: Methodology

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  14. In Arabidopsis thaliana we demonstrate that dying root hairs provide an easy and rapid in vivo model for the morphological identification of apoptotic-like programmed cell death (AL-PCD) in plants. The model desc...

    Authors: Bridget V Hogg, Joanna Kacprzyk, Elizabeth M Molony, Conor O'Reilly, Thomas F Gallagher, Patrick Gallois and Paul F McCabe

    Citation: Plant Methods 2011 7:45

    Content type: Methodology

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  15. Targeted Induced Loci Lesions IN Genomes (TILLING) is increasingly being used to generate and identify mutations in target genes of crop genomes. TILLING populations of several thousand lines have been generat...

    Authors: Seosamh Ó Lochlainn, Stephen Amoah, Neil S Graham, Khalid Alamer, Juan J Rios, Smita Kurup, Andrew Stoute, John P Hammond, Lars Østergaard, Graham J King, Phillip J White and Martin R Broadley

    Citation: Plant Methods 2011 7:43

    Content type: Methodology

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  16. To simulate expected future global warming, hexagonal arrays of infrared heaters have previously been used to warm open-field canopies of upland crops such as wheat. Through the use of concrete-anchored posts,...

    Authors: Muhammad Ishaq Asif Rehmani, Jingqi Zhang, Ganghua Li, Syed Tahir Ata-Ul-Karim, Shaohua Wang, Bruce A Kimball, Chuan Yan, Zhenghui Liu and Yanfeng Ding

    Citation: Plant Methods 2011 7:41

    Content type: Methodology

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  17. Accurate analysis of DNA methylation by bisulphite sequencing depends on the complete conversion of all cytosines into uracil. Until now there has been no standard or universal gene identified as an endogenous...

    Authors: Jing Wang, Chongnan Wang, Yan Long, Clare Hopkins, Smita Kurup, Kede Liu, Graham J King and Jinling Meng

    Citation: Plant Methods 2011 7:39

    Content type: Methodology

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  18. Complete organellar genome sequences (chloroplasts and mitochondria) provide valuable resources and information for studying plant molecular ecology and evolution. As high-throughput sequencing technology adva...

    Authors: Tongwu Zhang, Xiaowei Zhang, Songnian Hu and Jun Yu

    Citation: Plant Methods 2011 7:38

    Content type: Methodology

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  19. Plant hormones play a pivotal role in several physiological processes during a plant's life cycle, from germination to senescence, and the determination of endogenous concentrations of hormones is essential to...

    Authors: Maren Müller and Sergi Munné-Bosch

    Citation: Plant Methods 2011 7:37

    Content type: Methodology

    Published on:

  20. Transformation and subsequent regeneration of holoparasitic plants has never been reported, in part due to challenges in developing transformation protocols, but also because regeneration of obligate parasites...

    Authors: Mónica Fernández-Aparicio, Diego Rubiales, Pradeepa CG Bandaranayake, John I Yoder and James H Westwood

    Citation: Plant Methods 2011 7:36

    Content type: Methodology

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  21. To adjust their development to the environment, plants rely on specific signals that travel from shoot to root and vice versa. Here we describe an efficient micrografting protocol for Nicotiana attenuata, a usefu...

    Authors: Variluska Fragoso, Hannah Goddard, Ian T Baldwin and Sang-Gyu Kim

    Citation: Plant Methods 2011 7:34

    Content type: Methodology

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  22. Large-insert BAC and BIBAC libraries are important tools for structural and functional genomics studies of eukaryotic genomes. To facilitate the construction of BAC and BIBAC libraries and the transfer of comp...

    Authors: Xue Shi, Haiyang Zeng, Yadong Xue and Meizhong Luo

    Citation: Plant Methods 2011 7:33

    Content type: Methodology

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  23. The Arabidopsis thaliana-Pseudomonas syringae model pathosystem is one of the most widely used systems to understand the mechanisms of microbial pathogenesis and plant innate immunity. Several inoculation methods...

    Authors: Yasuhiro Ishiga, Takako Ishiga, Srinivasa R Uppalapati and Kirankumar S Mysore

    Citation: Plant Methods 2011 7:32

    Content type: Methodology

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  24. Microarray technology has matured over the past fifteen years into a cost-effective solution with established data analysis protocols for global gene expression profiling. The Agilent-016047 maize 44 K microar...

    Authors: Nanette Coetzer, Alexander A Myburg and Dave K Berger

    Citation: Plant Methods 2011 7:31

    Content type: Database

    Published on:

  25. Plant protoplasts, a proven physiological and versatile cell system, are widely used in high-throughput analysis and functional characterization of genes. Green protoplasts have been successfully used in inves...

    Authors: Yang Zhang, Jianbin Su, Shan Duan, Ying Ao, Jinran Dai, Jun Liu, Peng Wang, Yuge Li, Bing Liu, Dongru Feng, Jinfa Wang and Hongbin Wang

    Citation: Plant Methods 2011 7:30

    Content type: Methodology

    Published on:

  26. Plastids are small organelles equipped with their own genomes (plastomes). Although these organelles are involved in numerous plant metabolic pathways, current knowledge about the transcriptional activity of p...

    Authors: Agnieszka Żmieńko, Magdalena Guzowska-Nowowiejska, Radosław Urbaniak, Wojciech Pląder, Piotr Formanowicz and Marek Figlerowicz

    Citation: Plant Methods 2011 7:29

    Content type: Methodology

    Published on:

  27. Wheat is one of the most important crops in Australia, and the identification of young plants is an important step towards developing an automated system for monitoring crop establishment and also for differen...

    Authors: Mahmood R Golzarian and Ross A Frick

    Citation: Plant Methods 2011 7:28

    Content type: Methodology

    Published on:

  28. Total internal reflection fluorescence microscopy (TIRFM) is a powerful tool for observing fluorescently labeled molecules on the plasma membrane surface of animal cells. However, the utility of TIRFM in plant...

    Authors: Yinglang Wan, William M Ash III, Lusheng Fan, Huaiqin Hao, Myung K Kim and Jinxing Lin

    Citation: Plant Methods 2011 7:27

    Content type: Methodology

    Published on:

  29. We outline a high throughput procedure that improves outlier detection in cell wall screens using FT-NIR spectroscopy of plant leaves. The improvement relies on generating a calibration set from a subset of a ...

    Authors: Andreia M Smith-Moritz, Mawsheng Chern, Jeemeng Lao, Wing Hoi Sze-To, Joshua L Heazlewood, Pamela C Ronald and Miguel E Vega-Sánchez

    Citation: Plant Methods 2011 7:26

    Content type: Methodology

    Published on:

  30. Transient expression of proteins in plants has become a favoured method over the production of stably transformed plants because, in addition to enabling high protein yields, it is both fast and easy to apply....

    Authors: Dirk Stephan, Coba Slabber, Gavin George, Victor Ninov, Kevin P Francis and Johan T Burger

    Citation: Plant Methods 2011 7:25

    Content type: Methodology

    Published on:

  31. A method was developed to identify insertional mutants of Chlamydomonas reinhardtii disrupted for selected target genes. The approach relies on the generation of thousands of transformants followed by PCR-based s...

    Authors: David Gonzalez-Ballester, Wirulda Pootakham, Florence Mus, Wenqiang Yang, Claudia Catalanotti, Leonardo Magneschi, Amaury de Montaigu, Jose J Higuera, Matthew Prior, Aurora Galván, Emilio Fernandez and Arthur R Grossman

    Citation: Plant Methods 2011 7:24

    Content type: Methodology

    Published on:

  32. Prenylquinones are key compounds of the thylakoid membranes in chloroplasts. To understand the mechanisms involved in the response of plants to changing conditions such as high light intensity, the comprehensi...

    Authors: Jacopo Martinis, Felix Kessler and Gaetan Glauser

    Citation: Plant Methods 2011 7:23

    Content type: Methodology

    Published on:

  33. Cultivated sunflower (Helianthus annus L.) is a globally important oilseed crop, subjected to intensive genetic and genomic studies. Although classical mutagenesis has successfully been applied to Helianthus genu...

    Authors: Wilma Sabetta, Vittorio Alba, Antonio Blanco and Cinzia Montemurro

    Citation: Plant Methods 2011 7:20

    Content type: Research

    Published on:

  34. The biology of the seed is complicated by the extensive non-homogeneity (spatial gradients) in gene expression, metabolic conversions and storage product accumulation. The detailed understanding of the mechani...

    Authors: Silke Schiebold, Henning Tschiersch, Ljudmilla Borisjuk, Nicolas Heinzel, Ruslana Radchuk and Hardy Rolletschek

    Citation: Plant Methods 2011 7:19

    Content type: Methodology

    Published on:

  35. The tomato (Solanum lycopersicum L.) plant is both an economically important food crop and an ideal dicot model to investigate various physiological phenomena not possible in Arabidopsis thaliana. Due to the grea...

    Authors: Rogério F Carvalho, Marcelo L Campos, Lilian E Pino, Simone L Crestana, Agustin Zsögön, Joni E Lima, Vagner A Benedito and Lázaro EP Peres

    Citation: Plant Methods 2011 7:18

    Content type: Methodology

    Published on:

  36. The foundations for ethylene research were laid many years ago by researchers such as Lizada, Yang and Hoffman. Nowadays, most of the methods developed by them are still being used. Technological developments ...

    Authors: Inge Bulens, Bram Van de Poel, Maarten LATM Hertog, Maurice P De Proft, Annemie H Geeraerd and Bart M Nicolaï

    Citation: Plant Methods 2011 7:17

    Content type: Methodology

    Published on:

  37. Common bean (Phaseolus vulgaris L.) is a crop of economic and nutritious importance in many parts of the world. The lack of genomic resources have impeded the advancement of common bean genomics and thereby crop ...

    Authors: Claudia Díaz-Camino, Padmanaban Annamalai, Federico Sanchez, Aardra Kachroo and Said A Ghabrial

    Citation: Plant Methods 2011 7:16

    Content type: Methodology

    Published on:

  38. Virus-induced gene silencing (VIGS) is an effective technology for the analysis of gene functions in plants. Though there are many reports on virus vectors for VIGS in plants, no VIGS vectors available for Rosace...

    Authors: Shintarou Sasaki, Noriko Yamagishi and Nobuyuki Yoshikawa

    Citation: Plant Methods 2011 7:15

    Content type: Methodology

    Published on:

  39. Efficient high throughput screening systems of useful mutants are prerequisite for study of plant functional genomics and lots of application fields. Advance in such screening tools, thanks to the development ...

    Authors: Suk Weon Kim, Hye Jin Kim, Jong Hyun Kim, Yong Kook Kwon, Myung Suk Ahn, Young Pyo Jang and Jang R Liu

    Citation: Plant Methods 2011 7:14

    Content type: Methodology

    Published on:

  40. Safflower (Carthamus tinctorius L.) is a difficult crop to genetically transform being susceptible to hyperhydration and poor in vitro root formation. In addition to traditional uses safflower has recently emerge...

    Authors: Srinivas Belide, Luch Hac, Surinder P Singh, Allan G Green and Craig C Wood

    Citation: Plant Methods 2011 7:12

    Content type: Methodology

    Published on:

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