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  1. The detection and characterization of resistance reactions of crop plants against fungal pathogens are essential to select resistant genotypes. In breeding practice phenotyping of plant genotypes is realized b...

    Authors: Matheus Kuska, Mirwaes Wahabzada, Marlene Leucker, Heinz-Wilhelm Dehne, Kristian Kersting, Erich-Christian Oerke, Ulrike Steiner and Anne-Katrin Mahlein

    Citation: Plant Methods 2015 11:28

    Content type: Methodology

    Published on:

  2. Grass stalks architecturally support leaves and reproductive structures, functionally support the transport of water and nutrients, and are harvested for multiple agricultural uses. Research on these basic and...

    Authors: Sven Heckwolf, Marlies Heckwolf, Shawn M Kaeppler, Natalia de Leon and Edgar P Spalding

    Citation: Plant Methods 2015 11:26

    Content type: Methodology

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  3. Image analysis is increasingly used in plant phenotyping. Among the various imaging techniques that can be used in plant phenotyping, chlorophyll fluorescence imaging allows imaging of the impact of biotic or ...

    Authors: Céline Rousseau, Gilles Hunault, Sylvain Gaillard, Julie Bourbeillon, Gregory Montiel, Philippe Simier, Claire Campion, Marie-Agnès Jacques, Etienne Belin and Tristan Boureau

    Citation: Plant Methods 2015 11:24

    Content type: Methodology

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  4. Effects of abiotic and biotic stresses on plant photosynthetic performance lead to fitness and yield decrease. The maximum quantum efficiency of photosystem II (F v/F m) is a p...

    Authors: Justine Bresson, François Vasseur, Myriam Dauzat, Garance Koch, Christine Granier and Denis Vile

    Citation: Plant Methods 2015 11:23

    Content type: Methodology

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  5. Recently emerging approaches to high-throughput plant phenotyping have discovered their importance as tools in unravelling the complex questions of plant growth, development and response to the environment, bo...

    Authors: Jan F Humplík, Dušan Lazár, Tomáš Fürst, Alexandra Husičková, Miroslav Hýbl and Lukáš Spíchal

    Citation: Plant Methods 2015 11:20

    Content type: Methodology

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  6. Detection of induced mutations is valuable for inferring gene function and for developing novel germplasm for crop improvement. Many reverse genetics approaches have been developed to identify mutations in gen...

    Authors: Leonardo Galindo-González, David Pinzón-Latorre, Erik A Bergen, Dustin C Jensen and Michael K Deyholos

    Citation: Plant Methods 2015 11:19

    Content type: Methodology

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  7. Ion transport is a fundamental physiological process that can be studied non-invasively in living plants with radiotracer imaging methods. Fluoride is a known phytotoxic pollutant and understanding its transpo...

    Authors: Alexander K Converse, Elizabeth O Ahlers, Tom W Bryan, Jackson D Hetue, Katherine A Lake, Paul A Ellison, Jonathan W Engle, Todd E Barnhart, Robert J Nickles, Paul H Williams and Onofre T DeJesus

    Citation: Plant Methods 2015 11:18

    Content type: Research

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  8. Roots are vital to plants for soil exploration and uptake of water and nutrients. Root performance is critical for growth and yield of plants, in particular when resources are limited. Since roots develop in s...

    Authors: Ralf Metzner, Anja Eggert, Dagmar van Dusschoten, Daniel Pflugfelder, Stefan Gerth, Ulrich Schurr, Norman Uhlmann and Siegfried Jahnke

    Citation: Plant Methods 2015 11:17

    Content type: Methodology

    Published on:

  9. Biological fluids often have interesting and unusual physical properties to adapt them for their specific purpose. Laboratory-based rheometers can be used to characterise the viscoelastic properties of such fl...

    Authors: Catherine Collett, Alia Ardron, Ulrike Bauer, Gary Chapman, Elodie Chaudan, Bart Hallmark, Lee Pratt, Maria Dolores Torres-Perez and D Ian Wilson

    Citation: Plant Methods 2015 11:16

    Content type: Methodology

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  10. Terahertz technology is still an evolving research field that attracts scientists with very different backgrounds working on a wide range of subjects. In the past two decades, it has been demonstrated that ter...

    Authors: Ralf Gente and Martin Koch

    Citation: Plant Methods 2015 11:15

    Content type: Review

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  11. Plant phenotyping refers to a quantitative description of the plant’s anatomical, ontogenetical, physiological and biochemical properties. Today, rapid developments are taking place in the field of non-destruc...

    Authors: Achim Walter, Frank Liebisch and Andreas Hund

    Citation: Plant Methods 2015 11:14

    Content type: Review

    Published on:

  12. Water availability is a major limiting factor for wheat (Triticum aestivum L.) production in rain-fed agricultural systems worldwide. Root system architecture has important functional implications for the timing ...

    Authors: Cecile AI Richard, Lee T Hickey, Susan Fletcher, Raeleen Jennings, Karine Chenu and Jack T Christopher

    Citation: Plant Methods 2015 11:13

    Content type: Methodology

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  13. Three-dimensional canopies form complex architectures with temporally and spatially changing leaf orientations. Variations in canopy structure are linked to canopy function and they occur within the scope of g...

    Authors: Mark Müller-Linow, Francisco Pinto-Espinosa, Hanno Scharr and Uwe Rascher

    Citation: Plant Methods 2015 11:11

    Content type: Software

    Published on:

  14. Plant phenotype datasets include many different types of data, formats, and terms from specialized vocabularies. Because these datasets were designed for different audiences, they frequently contain language a...

    Authors: Anika Oellrich, Ramona L Walls, Ethalinda KS Cannon, Steven B Cannon, Laurel Cooper, Jack Gardiner, Georgios V Gkoutos, Lisa Harper, Mingze He, Robert Hoehndorf, Pankaj Jaiswal, Scott R Kalberer, John P Lloyd, David Meinke, Naama Menda, Laura Moore…

    Citation: Plant Methods 2015 11:10

    Content type: Methodology

    Published on:

  15. Field-based high throughput phenotyping is a bottleneck for crop breeding research. We present a novel method for repeated remote phenotyping of maize genotypes using the Zeppelin NT aircraft as an experimenta...

    Authors: Frank Liebisch, Norbert Kirchgessner, David Schneider, Achim Walter and Andreas Hund

    Citation: Plant Methods 2015 11:9

    Content type: Methodology

    Published on:

  16. The water status in plant leaves is a good indicator for the water status in the whole plant revealing stress if the water supply is reduced. The analysis of dynamic aspects of water availability in plant tiss...

    Authors: Said Dadshani, Andriy Kurakin, Shukhrat Amanov, Benedikt Hein, Heinz Rongen, Steve Cranstone, Ulrich Blievernicht, Elmar Menzel, Jens Léon, Norbert Klein and Agim Ballvora

    Citation: Plant Methods 2015 11:8

    Content type: Methodology

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  17. We review a set of recent multiscale imaging techniques, producing high-resolution images of interest for plant sciences. These techniques are promising because they match the multiscale structure of plants. H...

    Authors: David Rousseau, Yann Chéné, Etienne Belin, Georges Semaan, Ghassen Trigui, Karima Boudehri, Florence Franconi and François Chapeau-Blondeau

    Citation: Plant Methods 2015 11:6

    Content type: Review

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  18. The dietary carotenoids serve as precursor for vitamin A and prevent several chronic-degenerative diseases. The carotenoid profiling is necessary to understand their importance on human health. However, the av...

    Authors: Prateek Gupta, Yellamaraju Sreelakshmi and Rameshwar Sharma

    Citation: Plant Methods 2015 11:5

    Content type: Methodology

    Published on:

  19. Piercing-sucking insects are major vectors of plant viruses causing significant yield losses in crops. Functional genomics of plant resistance to these insects would greatly benefit from the availability of hi...

    Authors: Karen J Kloth, Cindy JM ten Broeke, Manus PM Thoen, Marianne Hanhart-van den Brink, Gerrie L Wiegers, Olga E Krips, Lucas PJJ Noldus, Marcel Dicke and Maarten A Jongsma

    Citation: Plant Methods 2015 11:4

    Content type: Methodology

    Published on:

  20. Well-developed and functional roots are critical to support plant life and reach high crop yields. Their study however, is hampered by their underground growth and characterizing complex root system architectu...

    Authors: Laura Mathieu, Guillaume Lobet, Pierre Tocquin and Claire Périlleux

    Citation: Plant Methods 2015 11:3

    Content type: Methodology

    Published on:

  21. During the last fifteen years, cell wall proteomics has become a major research field with the publication of more than 50 articles describing plant cell wall proteomes. The WallProtDB database has been designed ...

    Authors: Hélène San Clemente and Elisabeth Jamet

    Citation: Plant Methods 2015 11:2

    Content type: Database

    Published on:

  22. Combined assessment of leaf reflectance and transmittance is currently limited to spot (point) measurements. This study introduces a tailor-made hyperspectral absorption-reflectance-transmittance imaging (Hype...

    Authors: Sergej Bergsträsser, Dimitrios Fanourakis, Simone Schmittgen, Maria Pilar Cendrero-Mateo, Marcus Jansen, Hanno Scharr and Uwe Rascher

    Citation: Plant Methods 2015 11:1

    Content type: Methodology

    Published on:

  23. Detection and quantification of plant pathogens in the presence of inhibitory substances can be a challenge especially with plant and environmental samples. Real-time quantitative PCR has enabled high-throughp...

    Authors: Nejc Rački, Tanja Dreo, Ion Gutierrez-Aguirre, Andrej Blejec and Maja Ravnikar

    Citation: Plant Methods 2014 10:42

    Content type: Methodology

    Published on:

  24. Brassinosteriods (BRs) are a group of important phytohormones that have major effects on plant growth and development. To fully elucidate the function of BRs, a sensitive BR assay is required. However, most of...

    Authors: Jun Ding, Jian-Hong Wu, Jiu-Feng Liu, Bi-Feng Yuan and Yu-Qi Feng

    Citation: Plant Methods 2014 10:39

    Content type: Methodology

    Published on:

  25. In grasses, leaf growth is often monitored to gain insights in growth processes, biomass accumulation, regrowth after cutting, etc. To study the growth dynamics of the grass leaf, its length is measured at reg...

    Authors: Wannes Voorend, Peter Lootens, Hilde Nelissen, Isabel Roldán-Ruiz, Dirk Inzé and Hilde Muylle

    Citation: Plant Methods 2014 10:37

    Content type: Methodology

    Published on:

  26. The color of crop leaves is closely correlated with nitrogen (N) status and can be quantified easily with a digital still color camera and image processing software. The establishment of the relationship betwe...

    Authors: Yuan Wang, Dejian Wang, Peihua Shi and Kenji Omasa

    Citation: Plant Methods 2014 10:36

    Content type: Methodology

    Published on:

  27. Over the course of grape berry development, the tissues of the berry undergo numerous morphological transformations in response to processes such as water and solute accumulation and cell division, growth and ...

    Authors: Ryan J Dean, Timothy Stait-Gardner, Simon J Clarke, Suzy Y Rogiers, Gabriele Bobek and William S Price

    Citation: Plant Methods 2014 10:35

    Content type: Research

    Published on:

    The Erratum to this article has been published in Plant Methods 2016 12:7

  28. Studying gene evolution in non-model species by PCR-based approaches is limited to highly conserved genes. The plummeting cost of next generation sequencing enables the application of de novo transcriptomics to a...

    Authors: Achala S Jayasena, David Secco, Kalia Bernath-Levin, Oliver Berkowitz, James Whelan and Joshua S Mylne

    Citation: Plant Methods 2014 10:34

    Content type: Methodology

    Published on:

  29. Meiosis progression in the more recent past has been investigated using 5-bromo-2′-deoxyuridine (BrdU) uptake by S-phase meiocytes undergoing DNA replication. BrdU uptake is detected by reaction with BrdU anti...

    Authors: Patti E Stronghill, Wajma Azimi and Clare A Hasenkampf

    Citation: Plant Methods 2014 10:33

    Content type: Methodology

    Published on:

  30. Agroinfiltration-based transactivation systems can determine if a protein functions as a transcription factor, and via which promoter element. However, this activation is not always a yes or no proposition. No...

    Authors: Annette Nassuth, Mahbuba Siddiqua, Huogen Xiao, Michelle A Moody and Chevonne E Carlow

    Citation: Plant Methods 2014 10:32

    Content type: Research

    Published on:

  31. Ratiometric analysis with H+-sensitive fluorescent sensors is a suitable approach for monitoring apoplastic pH dynamics. For the acidic range, the acidotropic dual-excitation dye Oregon Green 488 is an excellent ...

    Authors: Christoph-Martin Geilfus, Karl H Mühling, Hartmut Kaiser and Christoph Plieth

    Citation: Plant Methods 2014 10:31

    Content type: Methodology

    Published on:

  32. The Arabidopsis root hair represents a valuable cell model for elucidating polar expansion mechanisms in plant cells and the overall biology of roots. The deposition and development of the cell wall is central to...

    Authors: Emily R Larson, Mary L Tierney, Berke Tinaz and David S Domozych

    Citation: Plant Methods 2014 10:30

    Content type: Methodology

    Published on:

  33. An important step in characterising the function of a gene is identifying the cells in which it is expressed. Traditional methods to determine this include in situ hybridisation, gene promoter-reporter fusions or...

    Authors: Asmini Athman, Sandra K Tanz, Vanessa M Conn, Charlotte Jordans, Gwenda M Mayo, Weng W Ng, Rachel A Burton, Simon J Conn and Matthew Gilliham

    Citation: Plant Methods 2014 10:29

    Content type: Methodology

    Published on:

  34. More than 90% of the Arabidopsis thaliana genes are members of multigene families. DNA sequence similarities present in such related genes can cause trouble, e.g. when molecularly analysing mutant alleles of thes...

    Authors: Gunnar Huep, Nils Kleinboelting and Bernd Weisshaar

    Citation: Plant Methods 2014 10:28

    Content type: Methodology

    Published on:

  35. Biofortification of staple crops with essential micronutrients relies on the efficient, long distance transport of nutrients to the developing seed. The main route of this transport in common wheat (Triticum aest...

    Authors: Lachlan James Palmer, Daniel Anthony Dias, Berin Boughton, Ute Roessner, Robin David Graham and JamesConstantine Roy Stangoulis

    Citation: Plant Methods 2014 10:27

    Content type: Methodology

    Published on:

  36. X-ray scattering is a well-established method for measuring cellulose microfibril angles in secondary cell walls. However, little data is available on the much thinner primary cell walls. Here, we show that mi...

    Authors: Friederike Saxe, Michaela Eder, Gunthard Benecke, Barbara Aichmayer, Peter Fratzl, Ingo Burgert and Markus Rüggeberg

    Citation: Plant Methods 2014 10:25

    Content type: Methodology

    Published on:

  37. Measuring grain characteristics is an integral component of cereal breeding and research into genetic control of seed development. Measures such as thousand grain weight are fast, but do not give an indication...

    Authors: Alex P Whan, Alison B Smith, Colin R Cavanagh, Jean-Philippe F Ral, Lindsay M Shaw, Crispin A Howitt and Leanne Bischof

    Citation: Plant Methods 2014 10:23

    Content type: Methodology

    Published on:

  38. Fluorescent proteins are extraordinary tools for biology studies due to their versatility; they are used extensively to improve comprehension of plant-microbe interactions. The viral infection process can easi...

    Authors: Fabio Pasin, Satish Kulasekaran, Paolo Natale, Carmen Simón-Mateo and Juan Antonio García

    Citation: Plant Methods 2014 10:22

    Content type: Methodology

    Published on:

  39. Next-generation sequencing technologies rely on high quality DNA that is suitable for library preparation followed by sequencing. Some plant species store large amounts of phenolics and polysaccharides within ...

    Authors: Adam Healey, Agnelo Furtado, Tal Cooper and Robert J Henry

    Citation: Plant Methods 2014 10:21

    Content type: Methodology

    Published on:

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