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Page 12 of 16

  1. Brassinosteriods (BRs), a group of important phytohormones, have various effects on plant growth and development. However, their physiological functions in plants have not been fully understood to date. Endoge...

    Authors: Jun Ding, Li-Jing Mao, Bi-Feng Yuan and Yu-Qi Feng

    Citation: Plant Methods 2013 9:13

    Content type: Methodology

    Published on:

  2. The hydraulic conductivity of the stem is a major factor limiting the capability of trees to transport water from the soil to transpiring leaves. During drought conditions, the conducting capacity of xylem can...

    Authors: Jussi-Petteri Suuronen, Marko Peura, Kurt Fagerstedt and Ritva Serimaa

    Citation: Plant Methods 2013 9:11

    Content type: Methodology

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  3. X-ray micro-Computed Tomography (μCT) offers the ability to visualise the three-dimensional structure of plant roots growing in their natural environment – soil. Recovery of root architecture descriptions from...

    Authors: Stefan Mairhofer, Susan Zappala, Saoirse Tracy, Craig Sturrock, Malcolm John Bennett, Sacha Jon Mooney and Tony Paul Pridmore

    Citation: Plant Methods 2013 9:8

    Content type: Software

    Published on:

  4. Heat stress and heat damage to plants gain globally increasing importance for crop production and plant survival in endangered habitats. Therefore the knowledge of heat tolerance of plants is of great interest...

    Authors: Othmar Buchner, Matthias Karadar, Ines Bauer and Gilbert Neuner

    Citation: Plant Methods 2013 9:7

    Content type: Methodology

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  5. Public genomic databases have provided new directions for molecular marker development and initiated a shift in the types of PCR-based techniques commonly used in plant science. Alongside commonly used arbitra...

    Authors: Péter Poczai, Ildikó Varga, Maarja Laos, András Cseh, Neil Bell, Jari PT Valkonen and Jaakko Hyvönen

    Citation: Plant Methods 2013 9:6

    Content type: Review

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  6. There are a number of unresolved issues in the design of experiments in greenhouses. They include whether statistical designs should be used and, if so, which designs should be used. Also, are there thigmomorp...

    Authors: Chris J Brien, Bettina Berger, Huwaida Rabie and Mark Tester

    Citation: Plant Methods 2013 9:5

    Content type: Methodology

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  7. Hydroponic growth systems are a convenient platform for studying whole plant physiology. However, we found through trialling systems as they are described in the literature that our experiments were frequently...

    Authors: Simon J Conn, Bradleigh Hocking, Maclin Dayod, Bo Xu, Asmini Athman, Sam Henderson, Lucy Aukett, Vanessa Conn, Monique K Shearer, Sigfredo Fuentes, Stephen D Tyerman and Matthew Gilliham

    Citation: Plant Methods 2013 9:4

    Content type: Methodology

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  8. Rhizo-lysimeters offer unique advantages for the study of plants and their interactions with soils. In this paper, an existing facility at Charles Sturt University in Wagga Wagga Australia is described in deta...

    Authors: Philip L Eberbach, Jeffrey Hoffmann, Sergio J Moroni, Leonard J Wade and Leslie A Weston

    Citation: Plant Methods 2013 9:3

    Content type: Methodology

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  9. Root growth is highly responsive to temporal changes in the environment. On the contrary, diel (24 h) leaf expansion in dicot plants is governed by endogenous control and therefore its temporal pattern does no...

    Authors: Tom Ruts, Shizue Matsubara and Achim Walter

    Citation: Plant Methods 2013 9:2

    Content type: Methodology

    Published on:

  10. Plant grafting techniques have deepened our understanding of the signals facilitating communication between the root and shoot, as well as between shoot and reproductive organs. Transmissible signalling molecu...

    Authors: Nazia Nisar, Shelley Verma, Barry J Pogson and Christopher I Cazzonelli

    Citation: Plant Methods 2012 8:50

    Content type: Methodology

    Published on:

  11. Quantification of callose deposits is a useful measure for the activities of plant immunity and pathogen growth by fluorescence imaging. For robust scoring of differences, this normally requires many technical...

    Authors: Ji Zhou, Thomas Spallek, Christine Faulkner and Silke Robatzek

    Citation: Plant Methods 2012 8:49

    Content type: Methodology

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  12. In this report we describe a chromatin immunoprecipitation (ChIP) protocol for two fully sequenced model diatom species Phaeodactylum tricornutum and Thalassiosira pseudonana. This protocol allows the extraction ...

    Authors: Xin Lin, Leïla Tirichine and Chris Bowler

    Citation: Plant Methods 2012 8:48

    Content type: Methodology

    Published on:

  13. Phytohormones are the key metabolites participating in the regulation of multiple functions of plant organism. Among them, jasmonates, as well as abscisic and salicylic acids are responsible for triggering and...

    Authors: Gerd Ulrich Balcke, Vinzenz Handrick, Nick Bergau, Mandy Fichtner, Anja Henning, Hagen Stellmach, Alain Tissier, Bettina Hause and Andrej Frolov

    Citation: Plant Methods 2012 8:47

    Content type: Methodology

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  14. Accurate characterization of complex plant phenotypes is critical to assigning biological functions to genes through forward or reverse genetics. It can also be vital in determining the effect of a treatment, ...

    Authors: Jason M Green, Heidi Appel, Erin MacNeal Rehrig, Jaturon Harnsomburana, Jia-Fu Chang, Peter Balint-Kurti and Chi-Ren Shyu

    Citation: Plant Methods 2012 8:45

    Content type: Methodology

    Published on:

  15. The male germline in flowering plants differentiates by asymmetric division of haploid uninucleated microspores, giving rise to a vegetative cell enclosing a smaller generative cell, which eventually undergoes...

    Authors: Filipe Borges, Rui Gardner, Telma Lopes, Joseph P Calarco, Leonor C Boavida, R Keith Slotkin, Robert A Martienssen and Jörg D Becker

    Citation: Plant Methods 2012 8:44

    Content type: Methodology

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  16. A large number of different plant lines are produced and maintained in a typical plant research laboratory, both as seed stocks and in active growth. These collections need careful and consistent management to...

    Authors: Jeroen Nieuwland, Emily Sornay, Angela Marchbank, Barend HJ de Graaf and James AH Murray

    Citation: Plant Methods 2012 8:43

    Content type: Software

    Published on:

  17. Cytokinins (CKs) are a group of plant growth regulators that are involved in several plant developmental processes. Despite the breadth of knowledge surrounding CKs and their diverse functions, much remains to...

    Authors: Scott C Farrow and RJ Neil Emery

    Citation: Plant Methods 2012 8:42

    Content type: Methodology

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  18. Deep sequencing is a powerful tool for novel small RNA discovery. Illumina small RNA sequencing library preparation requires a pre-adenylated 3’ end adapter containing a 5’,5’-adenyl pyrophosphoryl moiety. In ...

    Authors: Yun-Ru Chen, Yi Zheng, Bao Liu, Silin Zhong, Jim Giovannoni and Zhangjun Fei

    Citation: Plant Methods 2012 8:41

    Content type: Methodology

    Published on:

  19. Some prominent cultured plant cell lines, such as the BY-2 cell line of tobacco (Nicotiana tabacum cv. ‘Bright Yellow 2’) and the T87 cell line of Arabidopsis (Arabidopsis thaliana L. Heynh., ecotype Columbia) ar...

    Authors: Shinjiro Ogita, Taiji Nomura, Takao Kishimoto and Yasuo Kato

    Citation: Plant Methods 2012 8:40

    Content type: Methodology

    Published on:

  20. Phytophthora cinnamomi Rands. is an important root rot pathogen widely distributed in the north hemisphere, with a large host range. Among others diseases, it is known to be a principal factor in the decline of h...

    Authors: Francisco J Ruiz-Gómez, Rafael Sánchez-Cuesta, Rafael M Navarro-Cerrillo and Alejandro Pérez-de-Luque

    Citation: Plant Methods 2012 8:39

    Content type: Methodology

    Published on:

  21. Long distance signaling is a common phenomenon in animal and plant development. In plants, lateral organs such as nodules and lateral roots are developmentally regulated by root-to-shoot and shoot-to-root long...

    Authors: Tessema K Kassaw and Julia A Frugoli

    Citation: Plant Methods 2012 8:38

    Content type: Methodology

    Published on:

  22. Starch is the most important source of calories for human nutrition and the majority of it is produced by cereal farming. Starch is also used as a renewable raw material in a range of industrial sectors. It ca...

    Authors: Massimiliano Carciofi, Andreas Blennow, Morten M Nielsen, Preben B Holm and Kim H Hebelstrup

    Citation: Plant Methods 2012 8:36

    Content type: Methodology

    Published on:

  23. Although Single Nucleotide Polymorphism (SNP) marker is an invaluable tool for positional cloning, association study and evolutionary analysis, low SNP detection efficiency by Allele-Specific PCR (AS-PCR) stil...

    Authors: Jing Liu, Shunmou Huang, Meiyu Sun, Shengyi Liu, Yumei Liu, Wanxing Wang, Xiurong Zhang, Hanzhong Wang and Wei Hua

    Citation: Plant Methods 2012 8:34

    Content type: Methodology

    Published on:

  24. The plant hormone auxin, indole-3-acetic acid (IAA), plays important roles in plant growth and development. The signaling response to IAA is largely dependent on the local concentration of IAA, and this concen...

    Authors: Xing Liu, Adrian D Hegeman, Gary Gardner and Jerry D Cohen

    Citation: Plant Methods 2012 8:31

    Content type: Methodology

    Published on:

  25. Quantification of leaf movement is an important tool for characterising the effects of environmental signals and the circadian clock on plant development. Analysis of leaf movement is currently restricted by t...

    Authors: Ralph Bours, Manickam Muthuraman, Harro Bouwmeester and Alexander van der Krol

    Citation: Plant Methods 2012 8:29

    Content type: Methodology

    Published on:

  26. Sterols and Sphingolipids form lipid clusters in the plasma membranes of cell types throughout the animal and plant kingdoms. These lipid domains provide a medium for protein signaling complexes at the plasma ...

    Authors: Jörg O Blachutzik, Fatih Demir, Ines Kreuzer, Rainer Hedrich and Gregory S Harms

    Citation: Plant Methods 2012 8:28

    Content type: Methodology

    Published on:

  27. Understanding protein and gene function requires identifying interaction partners using biochemical, molecular or genetic tools. In plants, searching for novel protein-protein interactions is limited to protei...

    Authors: Kenneth Wayne Berendzen, Maik Böhmer, Niklas Wallmeroth, Sébastien Peter, Marko Vesić, Ying Zhou, Franziska KatharinaElisabeth Tiesler, Frank Schleifenbaum and Klaus Harter

    Citation: Plant Methods 2012 8:25

    Content type: Methodology

    Published on:

  28. Recent progress in cassava transformation has allowed the robust production of transgenic cassava even under suboptimal plant tissue culture conditions. The transformation protocol has so far been used mostly ...

    Authors: Ima M Zainuddin, Kim Schlegel, Wilhelm Gruissem and Hervé Vanderschuren

    Citation: Plant Methods 2012 8:24

    Content type: Methodology

    Published on:

  29. Cowpea (Vigna unguiculata L.) is an important grain and forage legume grown throughout sub-Saharan Africa primarily by subsistence farmers on poor, drought prone soils. Genetic improvement of the crop is being ac...

    Authors: Karolina E Mellor, Ava M Hoffman and Michael P Timko

    Citation: Plant Methods 2012 8:22

    Content type: Methodology

    Published on:

  30. Striga species are noxious root hemi-parasitic weeds that debilitate cereal production in sub-Saharan Africa (SSA). Control options for Striga are limited and developing Striga resistant crop germplasm is regarde...

    Authors: Steven Runo, Sarah Macharia, Amos Alakonya, Jesse Machuka, Neelima Sinha and Julie Scholes

    Citation: Plant Methods 2012 8:20

    Content type: Methodology

    Published on:

  31. Information about a transgene locus is one of the major concerns in transgenic research because expression of the transgene or a gene interrupted by the integration event could be affected. Thus, the flanking ...

    Authors: Joung Sug Kim, Jiye Kim, Tae-Ho Lee, Kyong Mi Jun, Tea Hoon Kim, Yul-Ho Kim, Hyang-Mi Park, Jong-Seong Jeon, Gynheung An, Ung-Han Yoon, Baek Hie Nahm and Yeon-Ki Kim

    Citation: Plant Methods 2012 8:19

    Content type: Software

    Published on:

  32. Microarrays are routine tools for transcript profiling, and genomic tiling arrays such as the Arabidopsis AGRONOMICS1 arrays have been found to be highly suitable for such experiments because changes in genome...

    Authors: Marlen Müller, Andrea Patrignani, Hubert Rehrauer, Wilhelm Gruissem and Lars Hennig

    Citation: Plant Methods 2012 8:18

    Content type: Methodology

    Published on:

  33. We have developed a new analytical approach for isolation and quantification of cytokinins (CK) in minute amounts of fresh plant material, which combines a simple one-step purification with ultra-high performa...

    Authors: Jana Svačinová, Ondřej Novák, Lenka Plačková, René Lenobel, Josef Holík, Miroslav Strnad and Karel Doležal

    Citation: Plant Methods 2012 8:17

    Content type: Methodology

    Published on:

  34. Seed transmission constitutes a major component of the parasitic cycle for several fungal pathogens. However, very little is known concerning fungal or plant genetic factors that impact seed transmission and m...

    Authors: Stephanie Pochon, Emmanuel Terrasson, Thomas Guillemette, Beatrice Iacomi-Vasilescu, Sonia Georgeault, Marjorie Juchaux, Romain Berruyer, Isabelle Debeaujon, Philippe Simoneau and Claire Campion

    Citation: Plant Methods 2012 8:16

    Content type: Methodology

    Published on:

  35. Genetic resources available for Arabidopsis thaliana make this species particularly attractive as a model for molecular genetic studies of guard cell homeostasis, transport and signalling, but this facility is no...

    Authors: Zhong-Hua Chen, Cornelia Eisenach, Xin-Qin Xu, Adrian Hills and Michael R Blatt

    Citation: Plant Methods 2012 8:15

    Content type: Methodology

    Published on:

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