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  1. The demand for effective use of water resources has increased because of ongoing global climate transformations in the agriculture science sector. Cost-effective and timely distributions of the appropriate amo...

    Authors: Adnan Zahid, Hasan T. Abbas, Aifeng Ren, Ahmed Zoha, Hadi Heidari, Syed A. Shah, Muhammad A. Imran, Akram Alomainy and Qammer H. Abbasi

    Citation: Plant Methods 2019 15:138

    Content type: Research

    Published on:

  2. Predicting impact of plant tissue culture media components on explant proliferation is important especially in commercial scale for optimizing efficient culture media. Previous studies have focused on predicti...

    Authors: Saeid Jamshidi, Abbas Yadollahi, Mohammad Mehdi Arab, Mohammad Soltani, Maliheh Eftekhari, Hamed Sabzalipoor, Abdollatif Sheikhi and Jalal Shiri

    Citation: Plant Methods 2019 15:136

    Content type: Research

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  3. Post-transcriptional gene regulation is one of the critical layers of overall gene expression programs and microRNAs (miRNAs) play an indispensable role in this process by guiding cleavage on the messenger RNA...

    Authors: Yong-Fang Li, Miao Zhao, Menglei Wang, Junqiang Guo, Li Wang, Jie Ji, Zongbo Qiu, Yun Zheng and Ramanjulu Sunkar

    Citation: Plant Methods 2019 15:134

    Content type: Methodology

    Published on:

  4. Due to its high damaging potential, Cercospora leaf spot (CLS) caused by Cercospora beticola is a continuous threat to sugar beet production worldwide. Breeding for disease resistance is hampered by the quantitat...

    Authors: Erich-Christian Oerke, Marlene Leucker and Ulrike Steiner

    Citation: Plant Methods 2019 15:133

    Content type: Research

    Published on:

  5. Unfortunately, the original version of the article [1] contained an error in figure 7. The names of species Umus minor and Acer campestre were interchanged. The corrected figure 7 is given below:

    Authors: Jorge Victorino and Francisco Gómez

    Citation: Plant Methods 2019 15:132

    Content type: Correction

    Published on:

    The original article was published in Plant Methods 2019 15:112

  6. Root and tuber crops are becoming more important for their high source of carbohydrates, next to cereals. Despite their commercial impact, there are significant knowledge gaps about the environmental and inher...

    Authors: Michael Gomez Selvaraj, Maria Elker Montoya-P, John Atanbori, Andrew P. French and Tony Pridmore

    Citation: Plant Methods 2019 15:131

    Content type: Research

    Published on:

  7. Enset (Ensete ventricosum), also known as Ethiopian banana, is a food security crop for more than 20 million people in Ethiopia. As conventional breeding of enset is very challenging, genetic engineering is an al...

    Authors: Jonathan Matheka, Jaindra Nath Tripathi, Ibsa Merga, Endale Gebre and Leena Tripathi

    Citation: Plant Methods 2019 15:130

    Content type: Methodology

    Published on:

  8. Tripterygium wilfordii Hook. f. (T. wilfordii) is an important medicinal plant with anti-inflammatory, immunosuppressive and anti-tumor activities. The main bioactive ingredients are diterpenoids and triterpenoid...

    Authors: Yadi Song, Shang Chen, Xiujuan Wang, Rui Zhang, Lichan Tu, Tianyuan Hu, Xihong Liu, Yifeng Zhang, Luqi Huang and Wei Gao

    Citation: Plant Methods 2019 15:129

    Content type: Research

    Published on:

  9. Non-contact resonant ultrasound spectroscopy (NC-RUS) has been proven as a reliable technique for the dynamic determination of leaf water status. It has been already tested in more than 50 plant species. In pa...

    Authors: María Dolores Fariñas, Daniel Jimenez-Carretero, Domingo Sancho-Knapik, José Javier Peguero-Pina, Eustaquio Gil-Pelegrín and Tomás Gómez Álvarez-Arenas

    Citation: Plant Methods 2019 15:128

    Content type: Research

    Published on:

  10. Sugar phosphates are important intermediates of central carbon metabolism in biological systems, with roles in glycolysis, the pentose–phosphate pathway, tricarboxylic acid (TCA) cycle, and many other biosynth...

    Authors: Umut Rende, Totte Niittylä and Thomas Moritz

    Citation: Plant Methods 2019 15:127

    Content type: Methodology

    Published on:

  11. Plants rely on concentration gradients of the native auxin, indole-3-acetic acid (IAA), to modulate plant growth and development. Both metabolic and transport processes participate in the dynamic regulation of...

    Authors: Federica Brunoni, Silvio Collani, Jan Šimura, Markus Schmid, Catherine Bellini and Karin Ljung

    Citation: Plant Methods 2019 15:126

    Content type: Methodology

    Published on:

  12. Lilium is one of the most popular flower crops worldwide, and some species are also used as vegetables and medicines. The availability of and easy access to diverse Lilium genetic resources are essential for plan...

    Authors: Jing-Wei Li, Xiao-Chen Zhang, Min-Rui Wang, Wen-Lu Bi, M. Faisal, Jaime A. Teixeira da Silva, Gayle M. Volk and Qiao-Chun Wang

    Citation: Plant Methods 2019 15:125

    Content type: Review

    Published on:

  13. Automated phenotyping technologies are continually advancing the breeding process. However, collecting various secondary traits throughout the growing season and processing massive amounts of data still take g...

    Authors: Jiating Li, Arun-Narenthiran Veeranampalayam-Sivakumar, Madhav Bhatta, Nicholas D. Garst, Hannah Stoll, P. Stephen Baenziger, Vikas Belamkar, Reka Howard, Yufeng Ge and Yeyin Shi

    Citation: Plant Methods 2019 15:123

    Content type: Research

    Published on:

  14. The number grain per panicle of rice is an important phenotypic trait and a significant index for variety screening and cultivation management. The methods that are currently used to count the number of grains...

    Authors: Wei Wu, Tao Liu, Ping Zhou, Tianle Yang, Chunyan Li, Xiaochun Zhong, Chengming Sun, Shengping Liu and Wenshan Guo

    Citation: Plant Methods 2019 15:122

    Content type: Research

    Published on:

  15. Despite wheat being a worldwide staple, it is still considered the most difficult to transform out of the main cereal crops. Therefore, for the wheat research community, a freely available and effective wheat ...

    Authors: Sadiye Hayta, Mark A. Smedley, Selcen U. Demir, Robert Blundell, Alison Hinchliffe, Nicola Atkinson and Wendy A. Harwood

    Citation: Plant Methods 2019 15:121

    Content type: Methodology

    Published on:

  16. A salient topic in developmental biology relates to the molecular and genetic mechanisms that underlie tissue morphogenesis. Modern quantitative approaches to this central question frequently involve digital c...

    Authors: Rachele Tofanelli, Athul Vijayan, Sebastian Scholz and Kay Schneitz

    Citation: Plant Methods 2019 15:120

    Content type: Methodology

    Published on:

  17. Targeted genome editing using the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/Cas9 system has been applied in a large number of plant species. Using a gene-specific single guide RNA (sgR...

    Authors: Xiucheng Cui, Margaret Balcerzak, Johann Schernthaner, Vivijan Babic, Raju Datla, Elizabeth K. Brauer, Natalie Labbé, Rajagopal Subramaniam and Thérèse Ouellet

    Citation: Plant Methods 2019 15:119

    Content type: Methodology

    Published on:

  18. Plant disease resistance to host-adapted pathogens is often mediated by host nucleotide-binding and leucine-rich repeat (NLR) receptors that detect matching pathogen avirulence effectors (AVR) inside plant cel...

    Authors: Isabel M. L. Saur, Saskia Bauer, Xunli Lu and Paul Schulze-Lefert

    Citation: Plant Methods 2019 15:118

    Content type: Methodology

    Published on:

  19. Assessing the impact of the environment on plant performance requires growing plants under controlled environmental conditions. Plant phenotypes are a product of genotype × environment (G × E), and the Envirat...

    Authors: Yin Bao, Scott Zarecor, Dylan Shah, Taylor Tuel, Darwin A. Campbell, Antony V. E. Chapman, David Imberti, Daniel Kiekhaefer, Henry Imberti, Thomas Lübberstedt, Yanhai Yin, Dan Nettleton, Carolyn J. Lawrence-Dill, Steven A. Whitham, Lie Tang and Stephen H. Howell

    Citation: Plant Methods 2019 15:117

    Content type: Research

    Published on:

  20. Virus diseases caused by co-infection with Sweet potato feathery mottle virus (SPFMV) and Sweetpotato chlorotic stunt virus (SPCSV) are a severe problem in the production of sweetpotato (Ipomoea batatas L.). Trad...

    Authors: Linping Wang, Sylvain Poque and Jari P. T. Valkonen

    Citation: Plant Methods 2019 15:116

    Content type: Research

    Published on:

  21. In situ hybridization (ISH) is a general molecular biological technique used to determine the spatiotemporal expression of genes in many species. In the past few years, numerous ISH protocols have been establi...

    Authors: Cheng Li, Simin Hu, Qidong Lei, Chongde Wang, Yunqiang Yang, Yongping Yang and Xudong Sun

    Citation: Plant Methods 2019 15:115

    Content type: Methodology

    Published on:

  22. Thorough understanding of complex model systems requires the characterisation of processes in different cell types of an organism. This can be achieved with high-throughput spatial transcriptomics at a large s...

    Authors: Michael Giolai, Walter Verweij, Ashleigh Lister, Darren Heavens, Iain Macaulay and Matthew D. Clark

    Citation: Plant Methods 2019 15:114

    Content type: Methodology

    Published on:

  23. In the original article [1], under the subheading “Image data processing”, last paragraph, last sentence that reads as “The least …… data collection” was incorrectly published. The correct sentence should read...

    Authors: Austin A. Dobbels and Aaron J. Lorenz

    Citation: Plant Methods 2019 15:113

    Content type: Correction

    Published on:

    The original article was published in Plant Methods 2019 15:97

  24. The categorical description of leaf shapes is of paramount importance in ecology, taxonomy and paleobotanical studies. Classification systems proposed by domain experts support these descriptions. Despite the ...

    Authors: Jorge Victorino and Francisco Gómez

    Citation: Plant Methods 2019 15:112

    Content type: Research

    Published on:

    The Correction to this article has been published in Plant Methods 2019 15:132

  25. Epicormic branches arise from dormant buds patterned during the growth of previous years. Dormant epicormic buds remain just below the surface of trees, pushed outward from the pith during secondary growth, bu...

    Authors: Mitchell Eithun, James Larson, Gregory Lang, Daniel H. Chitwood and Elizabeth Munch

    Citation: Plant Methods 2019 15:111

    Content type: Methodology

    Published on:

  26. Past research has shown that virus-induced phytoene desaturase (PDS) gene silencing via agroinjection in the attached and detached fruit of tomato plants results in a pale-yellow fruit phenotype. Although the PDS

    Authors: Aung Htay Naing, Swum Yi Kyu, Phyo Phyo Win Pe, Kyeung Il Park, Je Min Lee, Ki Byung Lim and Chang Kil Kim

    Citation: Plant Methods 2019 15:110

    Content type: Research

    Published on:

  27. As yields of major crops such as wheat (T. aestivum) have begun to plateau in recent years, there is growing pressure to efficiently phenotype large populations for traits associated with genetic advancement in y...

    Authors: Lorna McAusland, Jonathan A. Atkinson, Tracy Lawson and Erik H. Murchie

    Citation: Plant Methods 2019 15:109

    Content type: Methodology

    Published on:

  28. The growing field of plant molecular farming relies on expression vectors that allow high yields of recombinant proteins to be produced through transient gene expression. While numerous expression vectors curr...

    Authors: Hadrien Peyret, James K. M. Brown and George P. Lomonossoff

    Citation: Plant Methods 2019 15:108

    Content type: Research

    Published on:

  29. Plant breeders seek to develop cultivars with maximal agronomic value, which is often assessed using numerous, often genetically correlated traits. As intervention on one trait will affect the value of another...

    Authors: Mehdi Momen, Malachy T. Campbell, Harkamal Walia and Gota Morota

    Citation: Plant Methods 2019 15:107

    Content type: Methodology

    Published on:

  30. Type 2 Diabetes Mellitus has reached epidemic proportions as a result of over-nutrition and increasingly sedentary lifestyles. Current therapies, although effective, are not without limitations. These limitati...

    Authors: Chintha Lankatillake, Tien Huynh and Daniel A. Dias

    Citation: Plant Methods 2019 15:105

    Content type: Review

    Published on:

  31. The root is an important organ for water and nutrient uptake, and soil anchorage. It is equipped with root hairs (RHs) which are elongated structures increasing the exchange surface with the soil. RHs are also...

    Authors: Marjorie Guichard, Jean-Marc Allain, Michele Wolfe Bianchi and Jean-Marie Frachisse

    Citation: Plant Methods 2019 15:104

    Content type: Methodology

    Published on:

  32. Stalk lodging (breakage of plant stems prior to harvest) is a major problem for both farmers and plant breeders. A limiting factor in addressing this problem is the lack of a reliable method for phenotyping st...

    Authors: Douglas D. Cook, Witold de la Chapelle, Ting-Che Lin, Shien Yang Lee, Wenhuan Sun and Daniel J. Robertson

    Citation: Plant Methods 2019 15:102

    Content type: Methodology

    Published on:

  33. Fine root production is one of the least well understood components of the carbon cycle in terrestrial ecosystems. Minirhizotrons allow accurate and non-destructive sampling of fine root production. Small and ...

    Authors: Marie Arnaud, Andy J. Baird, Paul J. Morris, Angela Harris and Jonny J. Huck

    Citation: Plant Methods 2019 15:101

    Content type: Methodology

    Published on:

  34. With increasing pollution, herbicide application and interest in plant phenotyping, sensors capturing early responses to toxic stress are demanded for screening susceptible or resistant plant varieties. Standa...

    Authors: Anna Segečová, María Luisa Pérez-Bueno, Matilde Barón, Jan Červený and Thomas Georg Roitsch

    Citation: Plant Methods 2019 15:100

    Content type: Research

    Published on:

  35. We developed and characterised a highly mutagenised TILLING population of the barley (Hordeum vulgare) cultivar Golden Promise. Golden Promise is the ‘reference’ genotype for barley transformation and a primary o...

    Authors: Miriam Schreiber, Abdellah Barakate, Nicola Uzrek, Malcolm Macaulay, Adeline Sourdille, Jenny Morris, Pete E. Hedley, Luke Ramsay and Robbie Waugh

    Citation: Plant Methods 2019 15:99

    Content type: Methodology

    Published on:

  36. Hyperspectral imaging is emerging as a promising approach for plant disease identification. The large and possibly redundant information contained in hyperspectral data cubes makes deep learning based identifi...

    Authors: Koushik Nagasubramanian, Sarah Jones, Asheesh K. Singh, Soumik Sarkar, Arti Singh and Baskar Ganapathysubramanian

    Citation: Plant Methods 2019 15:98

    Content type: Research

    Published on:

  37. Iron deficiency chlorosis (IDC) is an abiotic stress in soybean [Glycine max (L.) Merr.] that causes significant yield reductions. Symptoms of IDC include interveinal chlorosis and stunting of the plant. While...

    Authors: Austin A. Dobbels and Aaron J. Lorenz

    Citation: Plant Methods 2019 15:97

    Content type: Research

    Published on:

    The Correction to this article has been published in Plant Methods 2019 15:113

  38. Stalk lodging is an impediment to improving profitability and production efficiency in maize. Lodging resistance, a comprehensive indicator to appraise genotypes, requires both characterization of mechanical p...

    Authors: Weiliang Wen, Shenghao Gu, Boxiang Xiao, Chuanyu Wang, Jinglu Wang, Liming Ma, Yongjian Wang, Xianju Lu, Zetao Yu, Ying Zhang, Jianjun Du and Xinyu Guo

    Citation: Plant Methods 2019 15:96

    Content type: Research

    Published on:

  39. Unfortunately, the original version of the article (1) contained an error. It has been brought to the attention of authors that the R108 ecotype was not derived from A17 but is rather a different subspecies de...

    Authors: Li Wen, Yuanling Chen, Elise Schnabel, Ashley Crook and Julia Frugoli

    Citation: Plant Methods 2019 15:95

    Content type: Correction

    Published on:

    The original article was published in Plant Methods 2019 15:20

  40. An effective and expeditious approach to assess plant nitrogen status is urgently needed in rice production and management as the conventional chemical methods are laborious and time-consuming.

    Authors: Ke Wu, Changwen Du, Fei Ma, Yazhen Shen, Dong Liang and Jianmin Zhou

    Citation: Plant Methods 2019 15:94

    Content type: Research

    Published on:

  41. Grafting, an ancient agronomic technique, is an artificial mode of asexual reproduction in plants. Recently, grafting research has gradually shifted from modifying agronomic traits to the study of molecular me...

    Authors: Yan Li, Wei Sun, Fulin Liu, Jin Cheng, Xiaojie Zhang, Hui Zhang and Yanxiu Zhao

    Citation: Plant Methods 2019 15:93

    Content type: Methodology

    Published on:

  42. Banana (Musa spp.) is the most popular marketable fruit crop grown all over the world, and a dominant staple food in many developing countries. Worldwide, banana production is affected by numerous diseases and pe...

    Authors: Michael Gomez Selvaraj, Alejandro Vergara, Henry Ruiz, Nancy Safari, Sivalingam Elayabalan, Walter Ocimati and Guy Blomme

    Citation: Plant Methods 2019 15:92

    Content type: Research

    Published on:

  43. Characterization and quantification of visual plant traits is often limited to the use of tools and software that were developed to address a specific context, making them unsuitable for other applications. Cover...

    Authors: Lianne Merchuk-Ovnat, Zev Ovnat, Orit Amir-Segev, Yaarit Kutsher, Yehoshua Saranga and Moshe Reuveni

    Citation: Plant Methods 2019 15:90

    Content type: Software

    Published on:

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