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Table 3 In vitro conservation of Passiflora spp.

From: Advances in micropropagation, somatic embryogenesis, somatic hybridizations, genetic transformation and cryopreservation for Passiflora improvement

Evaluated species

Aim of conservation

Preserved organ

Cryopreservation technique

References

P. pohlii Mast.

To develop a cryopreservation technique for the root system by using the V-Cryo-plate technique and characterize the anatomical alterations that occurred during the protocol's consecutive stage

Root

V-Cryo-plate technique

[101]

P. suberosa L.

To study the influence of explant age and exposure to the vitrification solutions PVS2 and PVS3 being evaluated. Furthermore, the occurrence of oxidative stress was analyzed at various phases of the protocol by measuring oxidative damage and the antioxidant defense enzymes

Shoot tip

V-Cryo-plate technique

[102]

P. pinnatistipula, P. tarminiana, P. mollissima

To develop cryopreservation protocols for recalcitrant or intermediate seeds which provide a viable method of long-term germplasm conservation

Seed

Encapsulation-cryopreservation technique

[104]

P. alata, P. cincinnata, P. coriacea, P. edulis, P. edulis f. flavicarpa, P. foetida, P. giberti, P. micropetala, P. morifolia, P. nitida)

To test germination percentage of 10 species

Seed

Liquid nitrogen (− 196 °C)

[107]

P. pohlii

To the development of in vitro preservation using axenic plant nodal segments

Nodal segments

Encapsulation-vitrification and vitrification techniques

[108]

P. edulis

to achieve the efficient cryopreservation of plant embryos by providing their rapid (5 min), uniform permeation by Plant Vitrification Solution cryoprotectant

Zygotic embryo

Vacuum infiltration vitrification (VIV),

[109]

P. eichleriana Mast., P. nitida Kunth., P. mucronata Lam.

To examine X-rays to check for any damage brought over by cryopreservation or defrosting

Seed

Liquid nitrogen (− 196 °C)

[111]