Evaluated species | Aim of conservation | Preserved organ | Cryopreservation technique | References |
---|---|---|---|---|
P. pohlii Mast. | To develop a cryopreservation technique for the root system by using the V-Cryo-plate technique and characterize the anatomical alterations that occurred during the protocol's consecutive stage | Root | V-Cryo-plate technique | [101] |
P. suberosa L. | To study the influence of explant age and exposure to the vitrification solutions PVS2 and PVS3 being evaluated. Furthermore, the occurrence of oxidative stress was analyzed at various phases of the protocol by measuring oxidative damage and the antioxidant defense enzymes | Shoot tip | V-Cryo-plate technique | [102] |
P. pinnatistipula, P. tarminiana, P. mollissima | To develop cryopreservation protocols for recalcitrant or intermediate seeds which provide a viable method of long-term germplasm conservation | Seed | Encapsulation-cryopreservation technique | [104] |
P. alata, P. cincinnata, P. coriacea, P. edulis, P. edulis f. flavicarpa, P. foetida, P. giberti, P. micropetala, P. morifolia, P. nitida) | To test germination percentage of 10 species | Seed | Liquid nitrogen (− 196 °C) | [107] |
P. pohlii | To the development of in vitro preservation using axenic plant nodal segments | Nodal segments | Encapsulation-vitrification and vitrification techniques | [108] |
P. edulis | to achieve the efficient cryopreservation of plant embryos by providing their rapid (5 min), uniform permeation by Plant Vitrification Solution cryoprotectant | Zygotic embryo | Vacuum infiltration vitrification (VIV), | [109] |
P. eichleriana Mast., P. nitida Kunth., P. mucronata Lam. | To examine X-rays to check for any damage brought over by cryopreservation or defrosting | Seed | Liquid nitrogen (− 196 °C) | [111] |