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Fig. 2 | Plant Methods

Fig. 2

From: A vector-free gene interference system using delaminated Mg–Al-lactate layered double hydroxide nanosheets as molecular carriers to intact plant cells

Fig. 2

Silencing of AtACTIN2 in Arabidopsis using LDH-NS-mediated dsRNA delivery. A The lift picture showed the Arabidopsis soaked in a solution containing LDH-dsRNAAtACTIN2. And put in the solid medium for a time gradient. AtACTIN2 transcription levels in five-day-old Arabidopsis seedlings after 0.5–24 h exposure to dsRNAAtACTIN2 in liquid medium. Control seedlings were soaked in media containing double distilled water only (ddW), dsRNAAtACTIN2 only (dsRNA), or LDH-NS only (LDH) for 24 h. B, C AtACTIN2 transcription levels in five-day-old Arabidopsis seedlings soaked in dsRNAAtACTIN2-supplemented liquid medium for B 0.5 h or C 6 h and then cultured on dsRNA-free solid media for 0–24 h. D The lift picture showed the Arabidopsis that had been injected with LDH-dsRNAAtACTIN2 or had all their tissues sprayed with it. LDH-dsRNAAtACTIN2. The tissue-specific transcription level of AtACTIN2 of mature Arabidopsis (leaf, stem, and flower) spray, injection for 24 h. The letters on the bars represent p < 0.05, which was calculated using Duncan's multiple range test to indicate statistically significant differences among the various groups

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Plant Methods

ISSN: 1746-4811