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Fig. 1 | Plant Methods

Fig. 1

From: A vector-free gene interference system using delaminated Mg–Al-lactate layered double hydroxide nanosheets as molecular carriers to intact plant cells

Fig. 1

A vector-free method for delivering dsRNA into intact plant cells. A Schematic summarizing the steps of the LDH-NS vector-free gene silencing method. 1. LDH-NSs are synthesized from Mg(C3H5O3)2·3H2O, Al(C3H5O3), and C3H6O3 using co-precipitation. 2. MgAl-LDH is delaminated to form LDH-NSs. 3. Design and in vitro synthesis of dsRNAs corresponding to target genes using T7 RNA polymerase. 4. Adsorption of the dsRNAs onto the LDH-NSs. 5. Delivery of the LDH-dsRNA complex to plant cells. 6. Penetration of the plant by the LDH-dsRNA complex, resulting in the silencing of the target gene. B AFM images of delaminated LDH-NSs at different titration temperatures; nanosheets color corresponds to vertical distance. Scale bars = 200 nm. C Agarose electrophoresis gels showing the adsorption of dsRNA onto LDH-NSs synthesized at different titration temperatures using various dsRNA:LDH mass ratios. Lanes: M, DL 2000 DNA marker; 1–10, dsRNA:LDH mass ratios of 1:1–1:10; -, control (dsRNA without LDH-NSs). D Time curve showing percent dsRNA adsorption onto LDH-NSs synthesized at different temperatures under conditions of dsRNA supersaturation

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Plant Methods

ISSN: 1746-4811