Table 2 Sequence of primers and probes for direct PCR and ddPCR assay
From: Scoring the number of B chromosomes in Zea mays L. using droplet digital PCR assay
Oligo ID | Modification and oligo sequence 5′–3′ |
|---|---|
Oligos used in direct PCR | |
B008_F | TAGTTCGTCTCCACACACGC |
B008_R | CGAGGAGGTCATCGTCATGG |
B432_F | ACATCCTGCTGAGCACAATCA |
B432_R | TAGCCTGTTCTGCCCTCTCA |
B452_F | TTGCCTTGTGCTGGATAGGG |
B452_R | GGGAACTGTAGCAGAGTCGG |
Oligos used in ddPCR | |
ZmADH1-AF123535_F | GAATGTGTGTTGGGTTTGCAT |
ZmADH1-AF123535_R | TACTGTACCTTCTTCGAATCTGCTG |
ZmADH1-AF123535_probe | VIC-TGCAGCCTAACCATGCGCAGGGTA-QSY |
B008_F | TAGTTCGTCTCCACACACGC |
B008_R | CGAGGAGGTCATCGTCATGG |
B008_probe | FAM-TCCTCCGCTCGACACATGTCCCTG-QSY |