Fig. 1
From: Scoring the number of B chromosomes in Zea mays L. using droplet digital PCR assay
Droplet fluorescence in ddPCR temperature gradient using digested and non-digested DNA. Digestion using HaeIII and different temperatures of annealing/extension step were tested in the temperature gradient analysis. The fluorescence of each droplet is shown as a dot on the graph, with colored droplets being positive (green for reference; blue for target) and grey being negative. Annealing/extension temperatures are shown below each sample (x-axis), separated by a red line. The undigested DNA showed a less focused signal for both reference (A) and target (B) probes. The HaeIII-digested DNA had a better signal-to-noise ratio in both reference (C) and target (D) channels