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Fig. 9 | Plant Methods

Fig. 9

From: Low-cost and automated phenotyping system “Phenomenon” for multi-sensor in situ monitoring in plant in vitro culture

Fig. 9

Exemplary depth data analysis of a culture vessel with four A. thaliana seedlings grown in vitro for 21 days (Trial A). A Yellow bar plot displays calculated media height (Mean ± SD), while black dots indicate sensor values of plant pixels after segmentation and colored boxplots indicate values for the calculation of the two digital parameters mean canopy height (green) and maximum plant height as mean of the upper 10 percentile (red). Red dashed line represents the maximum height of the sensor reliable distance (< = 72 mm) and the amount of plant pixels after segmentation was colorized in blue. B Individual plots of the dynamic behavior of the calculated digital parameters with equal color code and depicted as means ± SD. The calculation of medium volume and all other parameters is described in “Methods” section. 10,000 data points for each date were processed from depth scans of an area of 100 mm × 100 mm with a scan pattern of 1 mm × 1 mm. Depending on the necessary segmentation for the calculation of the individual parameters, a corresponding proportion of the 10,000 data points was included in the analysis

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