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Fig. 6 | Plant Methods

Fig. 6

From: Low-cost and automated phenotyping system “Phenomenon” for multi-sensor in situ monitoring in plant in vitro culture

Fig. 6

Monitoring of shoot regeneration of N. tabacum leaf explants. A RGB raw images and B processed images with the RGB imaging processing pipeline. N. tabacum leaf explants were placed on regeneration medium and developing adventitious shoot clusters were recorded over 32 days after treatment (DAT). Degree of coverage was calculated as the sum of plant pixels divided by total number of pixels within an image. For determination of explants area, sum of plant pixels was multiplied by pixel-metric-conversion factor. Time lapse video of N. tabacum regeneration is provided in Additional file 1

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Plant Methods

ISSN: 1746-4811