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Fig. 11 | Plant Methods

Fig. 11

From: Low-cost and automated phenotyping system “Phenomenon” for multi-sensor in situ monitoring in plant in vitro culture

Fig. 11

Exemplary determination of dynamic chlorophyll fluorescence monitoring of one of four A. thaliana seedlings grown in vitro for 21 days (Trial A). The first peak at 375 nm can be assigned to the excitation light provided by UV LEDs imperfectly blocked by the long pass filter at 420 nm (black long dashed line). The region from 400 to 660 nm has been masked for simplified representation. Emission peaks in the region from 660 to 780 nm indicated the two typical maxima of the chlorophyll fluorescence, derived from PSII (F690) and PSII and PSI (F730). Micro spectrometer integration time was set to 300 ms

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