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Table 1 Variables contained in the final master table of the digital image analysis

From: Workflow for phenotyping sugar beet roots by automated evaluation of cell characteristics and tissue arrangement using digital image processing

From computefeature

From preprocessing, processing, and clustering

Variable name

Description

Variable name

Description

s.area

Area size

Meandiameterµm

Diameter average calculated in µm

s.perimeter

Perimeter

Diameter_log

log10 of diameter

s.radius.mean

Mean radius

Q1

First quantile

s.radius.sd

Standard deviation of the mean radius

Q3

Third quantile

s.radius.max

Max radius

IQR

Interquartile range

s.radius.min

Min radius

nmbpeak

Number of peaks

m.cx

Center of mass x

nmdeep

Number of valleys

m.cy

Center of mass y

Cluster_drei

Classification of three clusters

m.majoraxis

Elliptical fit major axis

Cluster_zw

Classification of two clusters

m.eccentricity

Elliptical eccentricity

Imagetotalpix

Total image pixels

m.theta

Object angle

Innerpixarea

Total pixel ROI

order

Original order of cell sequence

Cellwallcount

Total pixel of cell wall

  

Intercellcount

Total pixel of cell spaces

  

Dataname

Image name

  1. The final master table comprises all relevant data generated at different points in the process. Morphometric variables were measured by the ComputeFeature function. These variables were used to calculate further variables during preprocessing, processing, and clustering. For each variable, the name and a description were included