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Fig. 9 | Plant Methods

Fig. 9

From: Fast and easy bioassay for the necrotizing fungus Botrytis cinerea on poplar leaves

Fig. 9

Scanning electron microscopy of Botrytis cinerea structures on inoculated Populus x canescens leaves af, hj and light microscopy of B. cinerea in liquid culture g. a Sterile PDA-plug inoculated leaf. b B. cinerea inoculated leaf. Edge of the necrotic (right) and healthy (left) area after four days. c) Collapsed cells of a B. cinerea inoculated leaf at 4 dpi (days post inoculation). d Mycelium growing from the plug to the leaf at 2 dpi. e) Spore on the abaxial side at 3 dpi. f Mycelium growing through a stoma at 3 dpi. g) B. cinerea spores germinated 24 h in ¼ strength Potato-Dextrose-Broth, h B. cinerea spores on inoculated leaf at 12 dpi, i germinated B. cinerea spores forming a hyphal network on poplar leaf at 12 dpi, j B. cinerea conidiophores at 12 dpi

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