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Table 2 Comparison between plant PacBio HiFi long-read sequencing results

From: A high quality, high molecular weight DNA extraction method for PacBio HiFi genome sequencing of recalcitrant plants

Taxon

DNA extraction method

Amount per cell (Gb)

Mean read length (kb)

References

(DNA extraction)

Zea mays

CTAB

24.1

15.6

Hon et al. 2020 [8]

(Doyle and Doyle 1987 [18])

Fragaria × ananassa

NI, Modified CTAB

29.7

21.7

Hon et al. 2020 [8]

(Li et al. 2020 [7])

Solanum lycopersicum

MN NucleoBond HMW DNA kit

20.3

18.3

van Rengs et al. 2022 [22]

Streptocarpus grandis

NI, CTAB, Qiagen Genomic-Tip

23.2

17.9

This study

Streptocarpus kentaniensis

NI, CTAB, Qiagen Genomic-Tip

22.6

15.6

This study

  1. Comparison between the current data and published plant PacBio HiFi datasets
  2. NI Nuclei isolation or similar filtration, MN Macherey–Nagel