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Fig. 1 | Plant Methods

Fig. 1

From: A high quality, high molecular weight DNA extraction method for PacBio HiFi genome sequencing of recalcitrant plants

Fig. 1

Quality of small scale DNA extractions using the protocol developed in this study. DNA was extracted in triplicates from two species of Streptocarpus, S. grandis and S. kentaniensis, using two different lysis conditions: 4H—4 h at 58 °C, ON—overnight at 50 °C. a Scatter plot of TapeStation Genomic DIN values (y-axis) versus obtained DNA in ng per g leaf sample (x-axis). b Scatter plot of spectrophotometer ratios, A260/A230 (A260vsA230) versus A260/A280 (A260vsA280). c Scatter plot of DNA concentration (ng/µl) quantified with fluorometer (Qubit_conc) versus spectrophotometer (Nanodrop_conc). d Scatter plot of Femto Pulse GQN analyses. GQN values (y-axis) indicate the proportion of the fragment quantity of the total DNA quantity of the GQNxkb threshold length (x-axis). For example, 50 on the x-axis indicates a fragment size of GQN50kb and the proportion of DNA quantity > 50 kb is shown on the y-axis. e TapeStation gel image of extracted DNA. Lanes: A1 ladder, B1-G1 S. grandis, H1-E2 S. kentaniensis, B1-D1 and H1-B2 ON lysis, E1-G1 and C2-E2 4H lysis

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