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Fig. 1 | Plant Methods

Fig. 1

From: Simultaneous determination of protoporphyrin IX and magnesium protoporphyrin IX in Arabidopsis thaliana and Camellia sinensis using UPLC-MS/MS

Fig. 1

Mobile phase optimization of UPLC-MS/MS analysis for protoporphyrin IX (PPIX) and Mg-protoporphyrin IX (Mg-PPIX). a, UPLC-MS/MS analysis using 0.1% methanol in water (v/v) and formic acid as mobile phases. The 1% formic acid leads to Mg-PPIX demetalation to PPIX. b, Chromogenic reaction of Mg-PPIX with different solvents. DMSO, Dimethyl sulfoxide. c, UPLC-MS/MS analysis of Mg-PPIX using different mobile phases. d, UPLC-MS/MS analysis of PPIX and Mg-PPIX using 0.1% ammonia (v/v) and 0.1% ammonium in acetonitrile (v/v) as mobile phase

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Plant Methods

ISSN: 1746-4811