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Fig. 5 | Plant Methods

Fig. 5

From: Peat-based hairy root transformation using Rhizobium rhizogenes as a rapid and efficient tool for easily exploring potential genes related to root-knot nematode parasitism and host response

Fig. 5

Expression of pCsLBD16::GUS pattern was activated in cucumber transgenic hairy roots during M. incognita parasitism. a Relative expression of CsLBD16 gene in mock-infection and nematode-infection roots during nematode parasitism. Student’s t-test was used for statistical analysis. n = 4. *P < 0.05, ***P < 0.001. b Contruction of pCAMBIA 1391 vector carrying the promoter region of CsLBD16. c Transgenic hairy roots harboring pCsLBD16::GUS expression pattern with mock-infection and M. incognita-infection at 7, 14, 21 and 28 DAI, Bar:500 μm. d Expression of the pCsLBD16::GUS reporter gene was strengthened in root galls around the head of female nematode. N, nematodes; Bar: 500 μm. e Fresh frozen sections of galls from pCsLBD16::GUS at 28 DAI showed GUS staining inside the pericycle cells around giant cells. Asterisks, GCs; N, nematodes; Bar: 200 μm. f Paraffin sections of cucumber transgenic hairy roots showed the concentrated GUS staining at the base of the lateral root primordium. Arrows indicated GUS staining in the lateral root primordium. Bar: 200 μm

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Plant Methods

ISSN: 1746-4811