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Fig. 4 | Plant Methods

Fig. 4

From: Peat-based hairy root transformation using Rhizobium rhizogenes as a rapid and efficient tool for easily exploring potential genes related to root-knot nematode parasitism and host response

Fig. 4

Editing of Malate synthase gene affected the establishment of feeding sites and the development of M. incognita in transgenic hairy roots. a Toluidine staining of paraffin Sects. (6-μm) of galls from the empty-vector control hairy roots and CRISPR/Cas9-guided CsMS knock-out hairy roots and photographed at 40 × magnification. Asterisks, GCs; N, Nematode; Bar:200 μm. b–e The proportion of J2(b), J3(c), J4(d) and female e nematodes to the total number of M. incognita in transgenic hairy roots at 14 DAI. f Proportion of giant cell area in root galls of transgenic hairy roots. g Numbers of giant cells per feeding site induced in transgenic hairy root. h–i Root galls and egg masses per mm of the empty-vector control hairy roots and CRISPR/Cas9-guided CsMS knock-out hairy roots after nematode infection. Data are presented as the mean ± SE, Student’s t-test, n ≥ 4. **p < 0.01; *p < 0.05

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Plant Methods

ISSN: 1746-4811