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Fig. 3 | Plant Methods

Fig. 3

From: Peat-based hairy root transformation using Rhizobium rhizogenes as a rapid and efficient tool for easily exploring potential genes related to root-knot nematode parasitism and host response

Fig. 3

CRISPR/Cas9-guided gene editing in cucumber transgenic hairy roots using the PCI method. a Relative expression of CsMS gene in mock-infection and nematode-infection roots during nematode parasitism. Student’s t-test was used for statistical analysis. n = 4. ***P < 0.001. b Schematic diagram for constructing two sgRNA-expressing cassettes in the binary vector pHEE401E. c Mutation sequencing in the cucumber transgenic hairy roots after CRISPR/Cas9-mediated gene editing

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Plant Methods

ISSN: 1746-4811