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Fig. 4 | Plant Methods

Fig. 4

From: Analysis of plant cuticles and their interactions with agrochemical surfactants using a 3D printed diffusion chamber

Fig. 4

SEM of surfactant treated isolated S. lycopersicum cuticular membranes. A–D transverse view of enzyme isolated CM treated with esterified vegetable oil (EVO), alcohol alkoxylate (AA), fatty acid ethoxylates (FAE) and organosilicone surfactant (OS). Surfactant deposited on the physiological outside 24 h prior to the experiment were found on the physiological inside of the CM. The deposits of surfactants AA and OS were clearly visible in the epidermal cell pockets. E–G represents physiological inside and H represents physiological outside of surfactant treated fruit cuticular membranes. With respect to EVO and AA the deposits filled epidermal cell pockets and looked dried up. FAE and OS deposits also filled the epidermal cell pockets but also remained as liquid as seen in image G. Occasional pitting was observed on the physiological outside of the CM treated with EVO as shown in image H. Scale bar 20 μm.

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