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Fig. 2 | Plant Methods

Fig. 2

From: Analysis of plant cuticles and their interactions with agrochemical surfactants using a 3D printed diffusion chamber

Fig. 2

The architecture of the enzymatically isolated S. lycopersicum cuticular membrane. Greyscale image A and B represents the top view of enzymatically isolated fruit CM under light microscopy and C and D represents top, bottom, and lateral view of fruit CM images obtained using an SEM. Images A, inset, and B represents images of fruit cuticle in increasing magnification at 2.5x, 40x, and 63× objective magnification. Images A and inset shows the transparent epicuticular wax and many irregular polygonal empty epidermal cell pockets lined with thick anticlinal walls. Solid white arrows in image B shows gaps- cuticular canals. Dashed white arrow shows anticlinal walls overhangs and dotted arrows shows anticlinal regions. Image C shows physiological outside (top) and inside (bottom) of fruit cuticle. Black arrow indicates a mechanical tear during isolation and white arrow indicates epidermal cell pockets. Image D shows the transverse section of the fruit cuticle showing epicuticular layer light grey in colour with hexagonal patterns (black arrow) and occasional trichome. The white arrow indicates darker anticlinal region, and three measurements indicate thickness of CM at different sections and width of an anticlinal region. Scale bar in µm for images A, inset, B, C, and D- 28, 500, 20, 100, and 20 respectively.

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