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Fig. 4 | Plant Methods

Fig. 4

From: Assembly and operation of an imaging system for long-term monitoring of bioluminescent and fluorescent reporters in plants

Fig. 4

Arabidopsis CCA1pro::LUC expression following entrainment to step and ramp thermocycle conditions. a Bioluminescent traces of ten-day old CCA1pro::LUC seedlings (grown under a 12 h:12 h light:dark, fluorescent lights, 22 °C in a growth room) that were entrained in a step temperature cycle (22 °C:12 °C for 12 h:12 h, constant light with PPFD of ~ 120 µmol m−2 s−1, constant ~ 25% relative humidity) for 5 days and then released into free-running conditions (constant 22 °C, constant light with PPFD of ~ 120 µmol m−2 s−1, constant ~ 25% relative humidity) for 5 days. Dark red and light red boxes represent warm and subjective warm hours, respectively. Error bars represent SD from at least 23 seedlings. Each seedling was normalized to its own average bioluminescence over time. bc Method-specific period (b) and method-specific circadian phase (c) of CCA1pro::LUC seedlings under step temperature cycle entrainment and free-running conditions, as reported by BioDare2 using FFT-NLLS or MESA analysis methods. Average period and phase are reported above data points. Error bars represent SD from at least 23 seedlings. For period analysis in BioDare2, “linear dtr” was selected for detrending, 18-34 h was selected for expected period, and “FFT NLLS” or “MESA” was selected for analysis method. d Bioluminescent traces of ten-day old CCA1pro::LUC seedlings (grown under a 12 h:12 h light:dark, fluorescent lights, 22 °C in a growth room) that were entrained in a ramp temperature cycle (22 °C:12 °C for 12 h:12 h, with 1-2 °C increments/h until reaching the set point, constant light with PPFD of ~ 120 µmol m−2 s−1, constant ~ 25% relative humidity) for 5 days and then released into free-running conditions (constant 22 °C, constant light with PPFD of ~ 120 µmol m−2 s−1, constant ~ 25% relative humidity) for 5 days. Error bars represent SD from 24 seedlings. Each seedling was normalized to its own average bioluminescence over time. ef Method-specific period (e) and method-specific circadian phase (f) of CCA1pro::LUC seedlings under ramp temperature cycle entrainment and free-running conditions, as reported by BioDare2 using FFT-NLLS or MESA analysis methods. Error bars represent SD from 24 seedlings. For period analysis in BioDare2, “linear dtr” was selected for detrending, 18-34 h was selected for expected period, and “FFT NLLS” or “MESA” was selected for the analysis method

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