Table 2 Comparison of results obtained from LAMP reactions and genotyping for identifying A) ALS and B) ACCase allelic variants in resistant Lolium spp. plants
Locus | Target mutation | Method | A) Population and plant numbers | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
651 | 670 | 678 | |||||||||||||||
1 | 2 | 3 | 4 | 5 | 1 | 2 | 3 | 4 | 5 | 1 | 2 | 3 | 4 | 5 | |||
ALS | 197 | LAMP | nd | nd | wt | nd | wt | nd | nd | nd | nd | nd | nd | wt | nd | mut | mut |
Genotyping | – | C(C/A)G | CAG | – | CAG | – | – | – | – | – | – | C(C/A)G | – | – | CAG | ||
376 | LAMP | wt | / | / | wt | wt | wt | wt | wt | nd | nd | wt | wt | wt | mut | nd | |
Genotyping | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | ||
574 | LAMP | nd | nd | nd | wt | wt | nd | nd | nd | wt | wt | wt | wt | wt | wt | wt | |
Genotyping | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | ||
Locus | Target mutation | Method | B) Population and plant numbers | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
651 | 576 | ||||||||||||||||
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 1 | 2 | 3 | 4 | 5 | |||
ACCase | 1781 | LAMP | nd | mut | wt | / | mut | mut | mut | wt | mut | wt | / | mut | nd | wt | mut |
Genotyping | (A/T)TA | (A/T)TA | – | / | TTA | (A/T)TA | (A/T)TA | – | (A/T)TA | – | TTA | (A/T)TA | (A/C)TA | - | (A/T)TA | ||
2041 | LAMP | wt | nd | mut | / | wt | nd | wt | wt | wt | mut | wt | wt | nd | wt | wt | |
Genotyping | – | A(T/A)T | A(T/A)T | / | – | A(T/G)T | – | A(T/A)T | – | AAT | – | – | A(T/A)T | – | – | ||
2078 | LAMP | nd | wt | nd | / | nd | wt | wt | mut | wt | wt | wt | / | nd | wt | mut | |
Genotyping | – | – | G(A/G)T | / | – | – | – | G(A/G)T | – | – | – | – | – | – | – | ||
| Â | Â | Â | 594 | 602 | 648 | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Â | Â | Â | 1 | 2 | 3 | 4 | 5 | 1 | 2 | 3 | 4 | 5 | 2 | 3 | 4 | 5 | 6 |
ACCase | 1781 | LAMP | mut | mut | wt | wt | mut | wt | wt | wt | mut | / | / | / | wt | nd | wt |
Genotyping | TTA | TTA | TTA | (A/T)TA | (A/T)TA | – | – | – | – | – | – | – | – | – | – | ||
2041 | LAMP | wt | nd | nd | wt | wt | wt | wt | wt | nd | wt | nd | mut | wt | wt | wt | |
Genotyping | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | ||
2078 | LAMP | wt | wt | wt | wt | wt | wt | wt | wt | wt | mut | wt | wt | nd | nd | mut | |
Genotyping | – | – | – | – | – | – | – | – | – | – | – | – | – | – | G(A/G)T | ||