Fig. 4

Dissecting cotyledons increases imaging accessibility without altering growth and cell divisions. Quantification of the segmentable area A and most lateral cell captured B from the three replicates in Fig. 3 at 4 DAS (squares) and 5 DAS (circles). Dissected samples (gray) trend towards or have significantly more segmentable area and furthest lateral cells exposed for imaging than undissected samples (blue). (Student’s t-tests, * = p < 0.05). C The largest total area captured for each condition at 4 DAS represented on its respective mesh. D The furthest lateral cell for each condition, undissected (blue) or dissected (gray), at 5 DAS shown on its respective mesh. Medial cells are also selected with a line drawn for distance reference. Note how the dissected sample’s most lateral cell is lower in the tissue so more marginal cells can be captured through dissection. E Cell areal growth from 4–5 DAS represented on the 4 and 5 DAS meshes for the sample with the median value of average growth for each condition. F Cell divisions from 4–5 DAS shown on the 5 DAS mesh for the sample with the median value of average divisions for each condition. The corresponding 4 DAS mesh is scaled and overlaid to show the parent cell outline for daughter cell clones. Insets are zoomed views. E–F Light- er shading indicates more growth (percent area increase) or divisions (#) as indicated. Average growth G or number of cell divisions H from 4–5 DAS for each replicate. Average growth and divisions are not statistically different between treatments, so dissection does not interfere with regular development (Student’s t-test p > 0.05). Black scale bars = 200 μm. See Table 1 for replicate cell counts.