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Fig. 3 | Plant Methods

Fig. 3

From: An optimized pipeline for live imaging whole Arabidopsis leaves at cellular resolution

Fig. 3

Dissecting cotyledons increases imaging accessibility. Comparison of exposed and segmentable cells in undissected (top three rows) versus dissected (bottom three rows) samples for the sample replicates from 4 DAS (A–C) to 5 DAS (D–F). More cells and more of the basal petiole and margin regions are accessible in the dissected samples. A, D Raw confocal images as maximum intensity projections. B, E Snapshots of the same images rendered as 2.5D meshes in MorphoGraphX 2.0. C, F Snapshots of the same meshes with segmentable cells indicated with unique colored labels and outlined in black. Red scale bars = 100 μm. Black scale bars = 200 μm

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